Kit for detecting furazolidone metabolin and method thereof

A technology for furazolidone and metabolites, which is applied in the field of kits for detecting furazolidone metabolites, can solve the problems that the detection limit of the detection method cannot be met, the processing process is complicated, the degree of instrumentation is high, and the detection time is short, the operation is simple, and the sensitivity is achieved. high effect

Active Publication Date: 2013-10-23
BEIJING KWINBON BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently the most commonly used methods for detecting furazolidone metabolites are high performance liquid chromatography (HPLC), liquid chromatography-mass chromatography (LC-MS, LC-MS / MS), etc. Although these methods have high sensitivity, accurate results, and repeatability Good, less false positives, etc., but there are still some disadvantages, such as complicated sample pretreatment process, high degree of instrumentation and high price, slow analysis speed, which has gradually failed to meet the detection limit requirements of developed countries and food processing enterprises.

Method used

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  • Kit for detecting furazolidone metabolin and method thereof
  • Kit for detecting furazolidone metabolin and method thereof
  • Kit for detecting furazolidone metabolin and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 detects the composition of the kit of furazolidone metabolite

[0042] 1. Test paper (called test strip) ( figure 1 )

[0043] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0044] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the bottom plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, and the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0045] The test paper is pasted with a protective film, and the protective film 7 is covered on the sample absorption pad, which is the detection end, and the word MAX is printed on it ( figure 2 );

[0046] There is a detection...

Embodiment 3

[0099] The detection of furazolidone metabolite in the animal tissue of embodiment 3

[0100] 1. Sample pretreatment

[0101]Take (5.0±0.05)g homogeneous sample to a 50ml polystyrene centrifuge tube, add 5ml 10% trichloroacetic acid, then add 100μl derivatization reagent (add methanol to the reagent bottle containing 151mg 2-nitrobenzaldehyde Dissolve to 10ml), shake fully for 3min, and place at 60°C for 1.5h; take out and add 1ml 0.5mol / L dipotassium hydrogen phosphate solution, 1.5ml 2mol / L sodium hydroxide solution and 10ml ethyl acetate, shake for 10s, Slightly shake the sample with high fat content for 8-10 times; centrifuge at room temperature (20-25°C) for 3000g or more; take 8ml of ethyl acetate phase into a 10ml dry glass test tube, and dry it in a water bath at 50-60°C under nitrogen flow. Add 1ml of n-hexane, vortex for 30s with a vortex, then add 0.6ml of 0.2mol / L phosphate buffer, vortex for 10s to mix thoroughly; above 3000g, centrifuge at room temperature (20-2...

Embodiment 4

[0108] The determination of embodiment 4 kit technical parameter

[0109] 1. Sensitivity test

[0110] The furazolidone metabolite standard (purchased from Sigma) was diluted to 0.5, 1, 2 μg / L; the diluent used was pH7.2, 0.2mol / L phosphate buffer.

[0111] Tested with the kit, the results are: when the concentration of the standard furazolidone metabolite is 0.5 μg / L, two red bands visible to the naked eye appear on the test strip, which is negative; the concentration of the standard furazolidone metabolite is 1 and 2 μg / L, the quality control area of ​​the test strip develops color, but the detection area does not develop color, which is positive, indicating that the sensitivity of this kit to detect furazolidone metabolites is 1 μg / L.

[0112] 2. False positive rate, false negative rate test

[0113] Take 20 positive samples of pork, chicken, fish, and shrimp with known furazolidone metabolite content greater than 1 μg / L and 20 negative samples of pork, chicken, fish, an...

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Abstract

The invention discloses a kit for detecting furazolidone metabolin and a method thereof. The kit comprises a micropore reagent and test paper, wherein a furazolidone metabolin specific antibody marked with collaurum is frozen in the micropore reagent; the furazolidone metabolin specific antibody can be furazolidone metabolin monoclonal antibody or furazolidone metabolin polyclonal antibody; the test paper is formed by successively connecting a sample absorption pad, a reaction membrane, a water absorption pad, a protection membrane and a bottom plate; and the reaction membrane comprises a detection area which is coated with furazolidone metabolin hapten-carrier protein conjugate and a quality control area which is coated with anti-antibody. The method for detecting the furazolidone metabolin by using the kit is simple, rapid, audio-visual, accurate, wide in application range, low in cost and easy for popularization and application.

Description

technical field [0001] The invention relates to a kit and a method for detecting furazolidone metabolites, in particular to a colloidal gold test strip for detecting furazolidone metabolites, which is particularly suitable for the detection of furazolidone metabolite residues in animal tissues. technical background [0002] Furazolidone (Furazolidone) belongs to nitrofuran drugs, which is a broad-spectrum antibiotic and has certain antibacterial effects on Gram-positive and negative bacteria. Prevention and treatment of chicken blackhead disease. However, studies have shown that nitrofuran drugs and their metabolites have considerable toxicity, teratogenic side effects, and can induce cancer. my country's Ministry of Agriculture document Nongmufa [2002] No. 1 stipulates that the detection limit of furazolidone in food animals is not to be detected. [0003] Since nitrofuran drugs can be metabolized quickly in the body, and the metabolites combined in tissues can persist fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/577
Inventor 何方洋罗晓琴赵正苗陈炜玲崔海峰汪善良扶胜杨秀贤
Owner BEIJING KWINBON BIOTECH
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