Complex microbial inoculant for broiler chickens and application thereof to improvement of broiler chicken productivity and immunity
A compound bacterial agent and broiler chicken technology, applied in the direction of application, microbial-based methods, biochemical equipment and methods, etc., can solve problems such as rough meat quality, lack of flavor, and overfat broiler carcasses
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Embodiment 1
[0077] Embodiment 1, the preparation of composite bacterial agent for broilers
[0078] This example relates to two kinds of composite bacterial agents for broilers, which are composite bacterial agents A for broilers and composite bacterial agents B for broilers.
[0079] Composite microbial agent A for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10 8 After cfu / ml, the mixture was mixed according to the colony-forming unit number ratio of 1:3:1.
[0080] The compound bacterial agent B for broilers is to dilute the liquid strains of Bacillus subtilis RJGP16, Saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 to 10% respectively with sterile water. 8 After cfu / m1, the mixture was mixed according to the colony-forming unit number ratio of 1:1:1.
[0081] Wherein, the liquid strain of Bacillus subtilis RJGP16 is a culture obtained by culturing with fermentat...
Embodiment 2
[0085] Embodiment 2, the preparation of broiler probiotics
[0086]This example provides two kinds of probiotics for broiler chickens, respectively called probiotics A for broilers and probiotics B for broilers.
[0087] The probiotics A for broilers is a culture obtained by using the fermentation medium A of Example 1 to cultivate the composite microbial agent A for broilers of Example 1. The specific preparation method is as follows: the broiler composite bacterial agent A of embodiment 1 is inserted into the 200L embodiment according to the inoculum amount (the volume of the composite bacterial agent inserted and the volume ratio of the fermentation medium) of 6% (volume ratio). In a 500L fermenter of fermentation medium A of 1, cultivate for 42 hours at 34°C, with an aeration rate of 600L / h and a stirring speed of 150rpm, collect all the cultured substances (fermentation liquid) in the fermenter, and add The rice husk powder was dried at 50° C. to obtain the probiotic pre...
Embodiment 3
[0089] Embodiment 3, improve broiler body weight with the broiler chicken probiotic A of embodiment 2
[0090] 1.1 Test animals
[0091] Select 12,000 1-day-old healthy chicks of Arboriga—Quick large white-feathered broiler chicken with a body weight of 50±5 grams, and divide them into 4 treatment groups at random, namely, the control group, the 0.015% probiotics group, and the 0.025% probiotics group. Ecological preparation group and 0.05% probiotic preparation group. There were 3 replicates per treatment group with 1000 chickens per replicate.
[0092] 1.2 Diet
[0093] Broiler brooding period diets were divided into broiler brooding period common rations, 0.015% broiler probiotics A brooding rations, 0.025% broiler probiotics A brooding rations, 0.05% broiler probiotics A brooding rations. Among them, the common diet for broiler brooding period is shown in Table 1; 0.015% broiler chicken probiotics A brooding period diet is added to the common diet for broiler chicken br...
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