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A process for producing ethanol by using microorganisms to ferment biomass

A biomass and alcohol technology, applied in the direction of microorganisms, microorganism-based methods, fermentation, etc., can solve the problems of high ethanol cost, high energy consumption, and low ethanol yield, so as to save the cost of enzyme preparations, shorten fermentation time, and improve The effect of utilization

Active Publication Date: 2015-08-26
临沂星火知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of fuel ethanol produced by biomass fermentation in the prior art is low, and the cost of ethanol produced is higher than other traditional methods
Second, there is no large-scale and industrialized production technology in China
The most fundamental reasons for the high cost are as follows: First, only high-temperature blasting can be used for raw material processing, which consumes a lot of energy.
The second is to consume a large amount of expensive enzyme preparations
The third is that the utilization rate is low due to the contamination of raw materials by bacteria
Fourth, the strain is single, the fermentation period is long, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment １

[0024] The first step is to pulverize the biomass (40% by weight of wheat straw, 40% by weight of corn stalk, and 20% by weight of rice straw), add water according to the mass ratio of biomass to water of 1:4, and pass through High-temperature steam treatment at 110-121°C for 40 minutes.

[0025] In the second step, Bacillus subtilis, Trichoderma reesei, Trichoderma lignin, Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Rhizopus, Candida utilis and Bacillus licheniformis were respectively activated and cultured, and expanded respectively. Cultivate, and then proportion according to weight fraction: 1.5 parts of Bacillus subtilis, 0.5 part of Trichoderma reesei, 0.6 part of Trichoderma lignin, 0.8 part of Trichoderma viride, 1 part of Aspergillus niger, 0.9 part of Aspergillus oryzae, 0.8 part of Rhizopus, 2 parts of Candida utilis, 1 part of Bacillus licheniformis, 1.2 parts by weight of magnesium sulfate, 0.9 parts by weight of calcium chloride and 1.5 parts by we...

Embodiment 2

[0031] In the first step, the biomass (30% by weight of wheat straw, 20% by weight of corn straw, 20% by weight of rice straw, 20% by weight of sorghum straw, and 10% by weight of bean straw) is pulverized, according to the After adding water at a mass ratio of substance to water of 1:5, pass through high-temperature steam at 110-121°C for 45 minutes.

[0032] In the second step, Bacillus subtilis, Trichoderma reesei, Trichoderma lignin, Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Rhizopus, Candida utilis and Bacillus licheniformis were respectively activated and cultured, and expanded respectively. Cultivate, and then proportion according to weight fraction: 0.5 part of Bacillus subtilis, 0.5 part of Trichoderma reesei, 0.5 part of Trichoderma lignin, 0.7 part of Trichoderma viride, 0.5 part of Aspergillus niger, 0.5 part of Aspergillus oryzae, 0.5 part of Rhizopus, 1.5 parts of Candida utilis, 0.5 parts of Bacillus licheniformis, 0.5 parts by weight of magnesi...

Embodiment 3

[0038] In the first step, the biomass (50% by weight of wheat straw, 40% by weight of corn stalk, and 10% by weight of sorghum stalk) is crushed, and water is added according to the mass ratio of biomass to water of 1:2. Into 110 ~ 121 ℃ high temperature steam treatment for 20min.

[0039] In the second step, Bacillus subtilis, Trichoderma reesei, Trichoderma lignin, Trichoderma viride, Aspergillus niger, Aspergillus oryzae, Rhizopus, Candida utilis and Bacillus licheniformis were respectively activated and cultured, and expanded respectively. Cultivate, and then proportion according to weight fraction: 1.5 parts of Bacillus subtilis, 1 part of Trichoderma reesei, 1 part of Trichoderma lignin, 1 part of Trichoderma viride, 1 part of Aspergillus niger, 1 part of Aspergillus oryzae, 1 part of Rhizopus, 3.6 parts of Candida utilis, 1.5 parts of Bacillus licheniformis, 1.2 parts by weight of magnesium sulfate, 1.2 parts by weight of calcium chloride and 2 parts by weight of sucros...

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Abstract

The invention provides a process for producing alcohol by using microorganisms to ferment biomass. The process first uses a cellulose treatment agent for treatment, and then mixes the cellulose treatment agent with Saccharomyces cerevisiae for fermentation production. Various agricultural wastes are used as fermentation raw materials, and a variety of microorganisms are mixed for fermentation. The raw material utilization rate is high and the alcohol production High efficiency, short fermentation cycle, low cost, suitable for industrial applications.

Description

technical field [0001] The invention relates to the technical field of alcohol production by fermentation, in particular to a process for producing alcohol by using microorganisms to ferment biomass (agricultural waste such as straw and rice stalks). Background technique [0002] At present, the energy shortage problem in the world is becoming more and more serious. my country imports a large amount of crude oil and energy products from abroad every year. At the same time, there is a large amount of various agricultural wastes every year, including rice straw, wheat straw, corn straw, soybean straw, sorghum straw, etc., 90% of which are not used. These stalks are used as raw materials to make fuel ethanol, which is a business of turning waste into wealth. [0003] At present, China's gasoline consumption is increasing at an average annual rate of 6%. In 2006, China's biofuel ethanol production was 1.32 million tons. But all are produced with grain as raw material. This h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/10C12R1/125C12R1/885C12R1/685C12R1/69C12R1/845C12R1/72C12R1/10C12R1/865
CPCY02E50/10
Inventor 张成如
Owner 临沂星火知识产权服务有限公司