Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation method for galactooligosaccharide composed of [-->5)beta-D-Galf(1-->] glycosyl

A technology of galactooligosaccharides and sugar groups, which is applied in the field of preparation of galactooligosaccharides, can solve the problems of high synthesis cost, high technical difficulty, and poor safety, and achieve high production efficiency, simplified preparation process, and no three wastes pollution Effect

Inactive Publication Date: 2013-12-11
TAISHAN MEDICAL UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of oligosaccharide synthesis, due to a series of problems related to the protection and deprotection of hydroxyl groups, the selective connection between sugar residues and the three-dimensional configuration encountered due to positioning modification, the technical difficulty is relatively large, the yield is low, and the synthesis High cost and poor security

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for galactooligosaccharide composed of [-->5)beta-D-Galf(1-->] glycosyl
  • Preparation method for galactooligosaccharide composed of [-->5)beta-D-Galf(1-->] glycosyl
  • Preparation method for galactooligosaccharide composed of [-->5)beta-D-Galf(1-->] glycosyl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] From [→5)β-D-Gal f (1→] the preparation method of the galactooligosaccharide of glycosyl composition, comprises the following steps:

[0030] (1) Degradation of polysaccharides: Prepare polysaccharides with [→5)β-D-Galf(1→] sugar groups as side chains and mannan as the main chain into an aqueous solution with a mass concentration of 1.0%. Add 0.1mol / L hydrochloric acid solution, adjust the pH value of the solution to 1.5, and hydrolyze in a constant temperature water bath at 20°C for 6 hours;

[0031] (2) Neutralization and concentration: neutralize the above solution with sodium hydroxide base until the pH value reaches 7.0, and concentrate the neutralized solution until the mass concentration of sugar is 10.0%;

[0032] (3) Alcohol precipitation: add the concentrated solution to 4 times the volume of alcohol, and carry out alcohol precipitation at 4°C for 12 hours to remove the undegraded mannose main chain;

[0033] (4) Centrifugation and concentration: Centrifuge ...

Embodiment 2

[0036] From [→5)β-D-Gal f (1→] the preparation method of the galactooligosaccharide of glycosyl composition, comprises the following steps:

[0037] (1) Degradation of polysaccharides: Prepare polysaccharides with [→5)β-D-Galf(1→] sugar groups as side chains and mannan as the main chain into an aqueous solution with a mass concentration of 7.0%. Add 0.55 mol / L sulfuric acid solution, adjust the pH value of the solution to 2.5, and hydrolyze in a constant temperature water bath at 55°C for 3.5 hours;

[0038] (2) Neutralization and concentration: neutralize the above solution with potassium hydroxide base until the pH value reaches 7.0, and concentrate the neutralized solution until the mass concentration of sugar is 10.0%;

[0039] (3) Alcohol precipitation: add the above-mentioned concentrated solution to ethanol with 4 times the volume of the concentrated solution, and alcohol precipitation at 14°C for 12 hours;

[0040] (4) Centrifugation and concentration: Centrifuge the...

Embodiment 3

[0043] From [→5)β-D-Gal f (1→] the preparation method of the galactooligosaccharide of glycosyl composition, comprises the following steps:

[0044] (1) Degradation of polysaccharides: Prepare polysaccharides with [→5)β-D-Galf(1→] sugar groups as side chains and mannan as the main chain into an aqueous solution with a mass concentration of 15%. Add 1.0 mol / L sulfurous acid solution, adjust the pH value of the solution to 3.5, and hydrolyze in a constant temperature water bath at 90°C for 1 hour;

[0045] (2) Neutralization and concentration: neutralize the above solution with ammonium hydroxide base until the pH value reaches 7.0, and concentrate the neutralized solution until the mass concentration of sugar is 10.0%;

[0046] (3) Alcohol precipitation: Add the above-mentioned concentrated solution to 4 times the volume of isopropanol of the concentrated solution, and alcohol precipitation at 25°C for 12 hours;

[0047] (4) Centrifugation and concentration: centrifuge the ab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
degree of polymerizationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a preparation method for galactooligosaccharide composed of [-->5)beta-D-Galf(1-->] glycosyl. The preparation method comprises the following steps: preparing an aqueous solution with a mass concentration of 1.0 to 15% from polysaccharide with [-->5)beta-D-Galf(1-->] glycosyl as a side chain and mannan as a main chain, adding an acid solution with a concentration of 0.1 to 1.0 mol / L with stirring, adjusting the pH value of the solution to 1.5 to 3.5 and carrying out hydrolysis at a constant temperature of 20 to 90 DEG C in a water bath for 1 to 6 h; carrying out alkali neutralization and alcohol precipitation to remove undegraded mannose main chains; centrifuging an obtained solution under the condition of 2000 to 6000 g for 10 to 30 min, subjecting an obtained supernatant to alcohol removal and concentrating the supernatant until the volume of the concentrated supernatant is one fifth of the original volume of the supernatant; and separating the concentrated supernatant by using low pressure column chromatography, carrying out detection by using a differential refraction detector, then carrying out combination and collection according to eluting peaks, removing a mobile phase and carrying out freeze drying. The preparation method provided by the invention has the advantages of simplified technological operation and improved preparation efficiency of oligosaccharide.

Description

technical field [0001] The invention relates to a preparation method of galactooligosaccharides composed of [→5)β-D-Galf(1→] sugar groups. Background technique [0002] Carbohydrates widely exist in nature, have very important biological activities, and play a very important role in many physiological and pathological processes. Among the saccharides found in nature, pyran-oligosaccharides are widely distributed, and their biological research and synthesis are developing very rapidly. Compared with pyran oligosaccharides, the research on furan oligosaccharides lags behind a lot. The biosynthetic process and metabolic mechanism of furan-type oligosaccharides have not been fully elucidated; the preparation of furan-type oligosaccharides is even rarer and immature. [0003] Oligosaccharides containing furan structure are mainly derived from bacteria, fungi, plants and protozoa. These oligosaccharides are important biomolecules that constitute the cell wall glycopeptides,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C13K13/00
Inventor 郭守东
Owner TAISHAN MEDICAL UNIV