Preparation method of traditional Chinese medicine effective part for treating endoxemia
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A technology of endotoxemia and effective parts, which is applied in the field of effective parts that can treat endotoxemia and its preparation, can solve the problems that have not been seen in the treatment of endotoxemia, and achieve low production costs, abundant natural resources, Strong regenerative effect
Inactive Publication Date: 2013-12-18
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology
So far, there is no report about the effective part of Jingsanleng in the treatment of endotoxemia
Method used
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Embodiment 1
[0023] Example 1 Preparation of effective fraction for anti-endotoxemia
[0024] (1) Extraction: take 20kg of dried tubers of Vitex trigonum, crush them, soak them with 3 times the amount of 85% ethanol aqueous solution for 1 day, then heat and reflux at 90°C for 2 hours, extract 2 times in total, and combine the 2 extracts .
[0025] (2) Column chromatography: Concentrate the extract obtained in step (1) under reduced pressure until it has no alcohol smell, dilute the concentrate with 1.5 times of water to form a suspension, and then extract with petroleum ether, ethyl acetate, and n-butanol respectively, The extracts from the ethyl acetate fractions were combined and concentrated to obtain 90.5 g of extract. The extract from the ethyl acetate part was subjected to normal-phase silica gel (100-200 mesh) column chromatography, and then eluted with several different elution solvents, each of which was composed of petroleum ether and ethyl acetate. And the volume ratios of pet...
Embodiment 2
[0027]Implementation example 2 In vivo anti-endotoxemia experiment of the effective part of jingsanleng
[0028] Experimental Materials
[0029] (1) Preparation of experimental drug solution: Precisely weigh two parts of the effective part of Jing Sanleng by weight reduction method, add appropriate amount of 0.5% CMC-Na solution respectively, and make two concentrations of drug solution with low and high concentrations.
[0030] (2) Endotoxin used in experiments: Escherichia coli lipopolysaccharide (LPS, 055: B5, Sigma, USA).
[0031] (3) Experimental animals: BALB / c female mice, purchased from Nanjing Qinglongshan Experimental Animal Center, certificate of conformity: SOXK (Su) 009-0001, weight range: 18-22 g.
[0032] experimental method
[0033] The administration method of this experiment is intragastric administration, and the modeling method is intraperitoneal injection of endotoxin (15mg / kg) to cause endotoxemia model. The specific experimental method is as follows. ...
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Abstract
The invention relates to a preparation method of a traditional Chinese medicine effective part for treating endoxemia, belonging to the technical field of Chinese medicine production. The preparation method comprises the following steps of: crushing dry scirpus yagara ohw tubers, adding 85% ethanol-water solution three times amount of the material, soaking for 24 hours, then carrying out reflux extraction for 2 times, wherein each reflux extraction lasts for 2 hours, filtering, combining extracting solutions and concentrating; adding water, the volume of which is 1.5 times of the volume of the concentrated extracting solution, to the concentrated extracting solution, respectively extracting by using extracting solvents, the polarities of which are from small to large, thus obtaining extract liquor, then concentrating, passing through a normal phase column for chromatography, and eluting by using an elution solvent; drying the collected eluant so as to obtain the scirpus yagara ohw effective part. According to the preparation method, the collected extracting solvent in the extract liquor is ethyl acetate, and the eluant solvent at least comprises an elution solvent formed by petroleum ether and ethyl acetate in a volume ratio of 100: 1, 100: 10 or 100: 12. Pharmacology experiments prove that the scirpus yagara ohw effective part prepared by the method has very good anti-endoxemia effect.
Description
technical field [0001] The present invention relates to the field of preparation and application of effective parts extracted from traditional Chinese medicines, in particular to an effective part obtained from the plant of Vitex trigonum and capable of treating endotoxemia and a preparation method thereof. Background technique [0002] Endotoxins are present in gram-negative bacteria (gram-negative bacteria, G - In the cell wall of bacteria), it is released when the cell wall disintegrates after the bacteria die, and the live bacteria can also release endotoxin in the form of blisters. Its chemical composition is lipopolysaccharide (lipopolysaccharide, LPS), the lipid of lipopolysaccharide A is the "virulence center" of LPS. A small amount of endotoxin has a certain significance in promoting the activation of the liver reticuloendothelial system, but when a large amount of endotoxin enters the blood circulation, it will stimulate the body's immune cells to release a large ...
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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/89A61P31/04A61K125/00
Inventor 梁侨丽崔晓东李军邹娜姝吕高虹袁冬平
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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