Pathogenic gene Prodh of cryphonectria parasitica and application thereof

Abstract

The invention discloses a pathogenic gene Prodh of cryphonectria parasitica and an application thereof. The gene contains two exons and one intron, has DNA (deoxyribonucleic acid) total length of 1585bp and cDNA (complementary DNA) total length of 1413bp and codes 438 amino acids. A protein expressed by the gene is shown in SEQ.ID.No.3 and is used for regulating and controlling pathogenesis of cryphonectria parasitica and a molecular mechanism of conidia production. Experiments prove that the sporulation quantity of a deletion mutant obtained after the gene is replaced by a hygromycin resistance gene hph is obviously reduced, and pathogenic scabs formed by the deletion mutant on in-vitro Chinese chestnut tree branches are obviously smaller than the pathogenic scabs produced by a wild type strain, thus sporulation quantity reduction and pathogenicity loss of cryphonectria parasitica are caused by deletion of the gene Prodh. The gene Prodh as well as an expression vector, a host and the like thereof has important significance in plant disease control drug and cryphonectria parasitica control study. Besides, transcription of the gene Prodh is inhibited after cryphonectria parasitica is infected with a hypovirus. The protein coded by the gene is probably a target after infection with the hypovirus. The mutual effects of the hypovirus and the gene have important significance in studying the molecular mechanism of virus-host interaction.

Description

technical field [0001] The invention belongs to the field of microbial genetic engineering and plant protection, and in particular relates to a Chestnut Phytophthora pathogenicity gene Prodh which affects fungal pathogenicity and sporulation and its application. Background technique [0002] Chestnut blight (Cryphonectria parasitica) is the pathogen that causes chestnut blight. It belongs to filamentous fungi in Ascomycetes in classification, and has now become a model fungus for studying the pathogenic mechanism of plant pathogenic fungi. [0003] At present, about 70% to 80% of plant diseases are caused by plant pathogenic fungi. Studying the infection process of pathogenic fungi is the basis for in-depth study of the pathogenic molecular mechanism of pathogenic fungi. According to the process of pathogenic fungi infecting the host, it is known that the pathogenic factors and genes of pathogenic fungi mainly include: (1) related to the adhesion of plant pathogenic fungi to...

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Problems solved by technology

Medication mainly relies on chemical agents, but the extensive use of chemical pesticides will bring negative impacts such as environmental pollution and ecological damage, and biological control has received more and more attention

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