Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Micro-fluidic chip and device for elegans cultivation and/or observation and application of micro-fluidic chip and device

A microfluidic chip and nematode technology, applied in the field of biotechnology and experimental equipment, can solve the problems of not being able to achieve cell or subcellular resolution, not being able to provide image data, and high cost of use

Active Publication Date: 2013-12-25
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI +1
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the COPAS system has obvious defects: it can only provide the total fluorescence value of the unit cross-section and the overall fluorescence value of the sample, and cannot reach the resolution of cells or subcellular levels; more importantly, the system cannot provide real-time visible image data ; In addition, the system equipment is expensive, and the cost of use is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-fluidic chip and device for elegans cultivation and/or observation and application of micro-fluidic chip and device
  • Micro-fluidic chip and device for elegans cultivation and/or observation and application of micro-fluidic chip and device
  • Micro-fluidic chip and device for elegans cultivation and/or observation and application of micro-fluidic chip and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0111] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. Those skilled in the art can make appropriate modifications and changes to the present invention, and these modifications and changes are all within the scope of the present invention. For example, the following examples illustrate the preparation method of microfluidic chips. In the examples, relatively simple photoresist SU-8, AZP 4620, AZ50XT and PDMS are used to prepare, but it does not mean that microfluidic chips can only be prepared by such methods. A skilled person can understand that any existing technology in the art can be used to prepare the microfluidic chip with the structure of the present invention.

[0112] For the experimental methods that do not indicate specific conditions in the following examples, conve...

preparation example 1

[0114] Preparation example 1. Adopt photolithography to make fluid layer pipeline (i.e. microfluidic pipeline) and control layer pipeline (i.e. control tube Tao) template

[0115] 1. Production of the control layer pipeline

[0116] The pipeline of the control layer is made of positive glue AZP 4620 (purchased from AZ Electronic Materials). Smoke the silicon wafer with hexamethyldisilane (HMDS) for 5 minutes, then pour positive resin AZP 4620 onto the silicon wafer modified by hexamethyldisilane, rotate at 1000rpm for 1 minute, and bake on a heating plate at 95°C Bake for 5 minutes, then bake on a 115°C heating plate for 10 minutes, and perform exposure and development according to conventional conditions.

[0117] 2. Fabrication of fluid layer pipelines

[0118] The production of the fluid layer pipeline is divided into three parts: the control part, the chamber part for cultivating nematodes, and the narrow pipeline for intercepting nematodes and flushing out eggs....

preparation example 2

[0127] Preparation Example 2. Fabrication of Microfluidic Chips and Devices

[0128] Experiments in Examples will be performed using a microfluidic chip and device having the following structures.

[0129] 1. Structure of the microfluidic chip

[0130] The structure of the microfluidic chip 1 is as follows: figure 1 Shown:

[0131] The nematode culture chamber 3 has a semicircular front (i.e. near the end of the input microfluidic pipeline) to prevent the food culture solution from being retained in a square dead corner. about).

[0132]There are 17 screening channels 30 with a width of 20 μm and a height of 10 μm at the rear of the culture chamber 3 (i.e., away from the end of the input microfluidic channel 4), which can prevent adult nematodes (about 90 μm in width) from passing through, but eggs and L1 to L3 (nematodes) The larval development stage is divided into four stages: L1, L2, L3 and L4, among which the length of L1 to L3 nematodes is 250-500 μm, and the wid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a micro-fluidic chip and a device for elegans cultivation and / or observation and application of the micro-fluidic chip and the device, and specifically provides the micro-fluidic chip for elegans cultivation, observation and / or research, the device comprising the micro-fluidic chip and the application of the micro-fluidic chip and the device. According to the micro-fluidic chip and the device, which are provided by the invention, high throughput and high resolution can be both considered, the growth of larvas is prevented from being restrained by the use of an additional chemical reagent, the manufacturing and using cost is low, the operation automation degree is high, and the application prospect is wide.

Description

technical field [0001] The invention relates to the fields of biotechnology and experimental equipment. Specifically, the present invention relates to a nematode culture microfluidic chip that is cheap, easy to prepare, and can automatically wash away larvae and eggs, a device containing the microfluidic chip, and applications of the microfluidic chip and the device. Background technique [0002] Caenorhabditis elegans is an important model material for modern developmental biology, genetics and genomics research. It is a very small worm whose adult body is only 1mm long, transparent all over, feeds on bacteria, lives in the soil, and has a short life cycle. It only takes a little more than two days to develop from a fertilized egg to a mature adult (at 25°C it takes 52 hours, the fastest can reach 48 hours). Cell division and cell lineage formation in wild-type C. elegans embryonic development are highly programmed, which facilitates genetic analysis of their development....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033B01L3/00
Inventor 韩敬东黄岩谊申洁鲜波
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com