Protein chip kit marked by quantum dot and preparation method thereof
A protein chip and quantum dot technology, applied in the field of biomedical detection, can solve the problems of high requirements on detection equipment, prone to cross-contamination, unsuitable for multi-step reactions, etc., to achieve high labeling efficiency, improved sensitivity and accuracy, The effect of high sensitivity and accuracy
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[0027] This embodiment takes cardiovascular disease inflammatory factors as an operation example to describe in detail the quantum dot-labeled protein chip kit and its preparation method. Inflammatory factors of cardiovascular disease include CRP (C-reactive protein), Myoglobin (myoglobin), cTnT (cardiac troponin T), NT-proBNP (N-terminal B-type natriuretic peptide), CK-MB (creatine kinase isozyme), H-FABP (heart-type fatty acid binding protein), GPBB (glycogen phosphorylase isozyme BB) and D-Dimer (D-dimer), a total of 8 biomarkers. The specific conditions and experimental steps not indicated in the examples were carried out according to the prior art.
[0028] EDC is 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride; NHS is N-hydroxysuccinimide; PBS is phosphate buffered saline; BSA is bovine serum albumin; PBST Phosphate Tween buffer.
[0029] (1) Preparation of solid phase carrier
[0030] Cleaning: 10% Na 2 CO 3 Spread the solution on the surface of the glas...
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