Protein chip kit marked by quantum dot and preparation method thereof

Abstract

The invention belongs to the technical field of biomedical detection and particularly relates to a protein chip kit marked by a quantum dot and a preparation method thereof. The protein chip kit marked by the quantum dot comprises a solid-phase carrier, a solid-phase antibody and detection liquid, wherein the solid-phase carrier is composed of a slide, an amino modified layer, an aldehyde modified layer and an agarose modified layer; the detection liquid is an antibody marked by the quantum dot activated by EDC / HNS (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride / N-Hydroxysuccinimide). According to the structure of the solid-phase carrier and the preparation method, the solid-phase antibody can be stably fixed in a sample application hole and the evaporation of the solid-phase antibody is reduced, so that the reaction capacity is improved and the activity is kept; five times of sample application are repeatedly carried out on each type of sample application samples so that the reaction capacity can be further improved and the accuracy of a detection result can also be improved; negative control markers and positive control markers are fixedly arranged on four corners of the solid-phase carrier so that the accuracy of the detection result can be improved. According to the preparation method of a detection agent, the marking efficiency of the quantum dot is high, and the stability and the activity of the marked antibody are high, so that the detection sensitivity and the detection accuracy can be improved and the production cost of the kit is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to a quantum dot-labeled protein chip kit and a preparation method thereof. Background technique [0002] The concept of protein chip technology originated in the late 1980s and is one of the most popular new technologies in the field of life sciences. The protein chip has the advantages of parallelism, high throughput, high sensitivity, less sample consumption, and fast detection speed. However, due to the complex and changeable chemical composition and structure of proteins, the proteins immobilized on the chip surface are easily denatured and inactivated. Therefore, finding a suitable carrier and surface modification method to ensure the immobilization of a sufficient amount of protein and maintain its natural activity has become one of the key steps in the development of protein microarrays. [0003] In recent years, through the attempts and research and developme...

AI Technical Summary

Problems solved by technology

However, immobilizing proteins with glass slides often has disadvantages such as easy evaporation of samples, unsuitable for multi-step reactions, small capacity, and prone to cross-contamination.

[0004] In addition, in the process of making protein chips, the use of traditional organic dyes as detection markers often brings disadvantages such as low sensitivity, poor stability, and high requirements for detection equipment.

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