Cladophora comprehensive utilization technology

The invention relates to a technology of Phytophthora algae and technology, which is applied in the field of comprehensive development and utilization of Phytolithiasis algae, and can solve the problems of high toughness of algae body, thick algae cell wall, and difficulty in decomposition.

Inactive Publication Date: 2014-01-08
YANTAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The cell wall of Cladophora is extremely thick, the algae body is tough, and it is difficult to decompose under natural conditions. A large amount of algae body fished from the water body is accumulated on the bank of th

Method used

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  • Cladophora comprehensive utilization technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Cladoella is harvested and dried (1), then mechanically pulverized, and passed through a 10-100 mesh sieve (2). Add 1-5% dilute sulfuric acid (3) according to the solid-to-liquid ratio of 1:5-1:20, heat at 50-100°C for 10-90min (4), filter and separate (5), and obtain the treatment liquid (6) and Cladophora Processed residue (7).

[0017] Adjust the pH of the treatment solution (6) to 3.5-7.0 (8), add an appropriate amount of ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, etc. (9), insert the activated yeast (10), and inoculate the amount of 1- 10%, cultured at 20-35° C. for 4-20 hours (11), centrifuged (12), and separated to obtain feed yeast (13), whose protein content is higher than 45%.

[0018] Adjust the pH of the residue (7) to 4.5-5.5 (14), add 10-100 times of water, add 10-200U / g cellulase (15), and enzymatically hydrolyze at 30-60°C for 1-40h (16) , separated by filtration (17), to obtain the enzymolysis liquid (18) an...

Embodiment 2

[0022] Cladoella is harvested and dried (1), then mechanically pulverized, and passed through a 10-100 mesh sieve (2). Add 2-5% sodium hydroxide solution (3) according to the solid-to-liquid ratio of 1:5-1:30, heat at 50-100°C for 10-90min (4), and centrifuge (5) to obtain the treatment liquid (6) and bristles Residue after algae treatment (7).

[0023] Adjust the pH of the treatment solution (6) to 3.5-7.0 (8), add an appropriate amount of ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, etc. (9), insert the activated yeast (10), and inoculate the amount of 1- 20%, cultured at 20-35° C. for 4-20 hours (11), flocculated and separated (12), and after separation, feed yeast (13) was obtained, and the protein content was higher than 47%.

[0024] Adjust the pH of the residue (7) to 4.5-5.5 (14), add 10-100 times of water, add 10-100U / g cellulase (15), and enzymatically hydrolyze at 30-60°C for 1-40h (16) , separated by filtration (17), to o...

Embodiment 3

[0028] Cladoella is harvested and dried (1), then mechanically pulverized, and passed through a 10-100 mesh sieve (2). Add 2-5% sodium hydroxide solution (3) according to the solid-to-liquid ratio of 1:5-1:30, heat at 50-100°C for 10-90min (4), filter and separate (5), and obtain the treatment liquid (6) and bristles Residue after algae treatment (7).

[0029] Adjust the pH of the treatment solution (6) to 3.5-7.0 (8), add an appropriate amount of ammonium sulfate, potassium dihydrogen phosphate, magnesium sulfate, calcium chloride, etc. (9), insert the activated yeast (10), and inoculate the amount of 1- 20%, cultured at 20-35°C for 4-20h (11), centrifuged (12) to obtain feed yeast (13), the protein content of which is higher than 45%.

[0030] Adjust the pH of the residue (7) after the treatment of Cladophora to 4.5-5.5 (14), add 10-100 times the water, add 10-200U / g cellulase (28), and inoculate the mold (29) at the same time, 25- Cultivate at 35° C. for 10-24 hours (30),...

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Abstract

The invention relates to a cladophora comprehensive development and utilization technology, and relates to a comprehensive development and utilization technology for producing bioethanol and high-protein feed by cladophora while reducing discharge of 'three waste'. After harvesting, cladophora is dried, crushed, and treated by acid or base; a proper amount of nutritional salts are added in the treatment fluid, which can directly used for yeast culture to gain feed yeast with high protein content; A proper amount of cellulase is added into residues obtained after treatment; yeast or mould is inoculated to obtain feed with high protein content; or the residues obtained after treatment is subjected to enzymolysis by adding cellulose; the enzymolysis liquid can be used for ethanol fermentation; residues obtained after enzymolysis is added with a proper amount of wheat bran; and mould or yeast is inoculated to obtain feed with high protein content. The technology of the invention is simple in required equipment, safe in operation, high in utilization rate of the raw material of cladophora, and low in 'three waste' pollution.

Description

technical field [0001] The invention relates to a comprehensive development and utilization technology of Clado algae, which is a comprehensive utilization and development technology for producing biological ethanol and high-protein feed and reducing discharge of three wastes by utilizing Clado algae. Background technique [0002] Cladophora (hollow moss), also commonly known as "steelgrass, clastica", is a common green algae, annual or perennial. The plant body is a multicellular branched filamentous body that attaches to and grows on the substrate; some elderly individuals float due to detachment from the attachment; Clado algae has a large amount of resources and grows rapidly, which is one of the main diseases of pond culture. Because its growth system is very developed, it can reproduce and grow rapidly in a relatively short period of time. It will soon occupy a large part of the breeding space in the breeding pond, absorb a large amount of nutrients in the bottom and w...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12P7/10A23K1/00C12R1/865
CPCY02E50/16Y02E50/10
Inventor 姜爱莉杨楠楠牛鹏军
Owner YANTAI UNIV
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