Supercharge Your Innovation With Domain-Expert AI Agents!

Organic-inorganic complex lipid ultrasonography microcapsule and preparation method thereof

A compound lipid and microcapsule technology, applied in the field of biomedical materials, can solve problems such as unreported, and achieve the effects of easy availability of raw materials, easier size control, and simple operation process

Inactive Publication Date: 2014-01-29
SUN YAT SEN UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on organic-inorganic composite lipid vesicles is mainly in the aspects of drug carrier and gene transfection, but there is no report on its application in ultrasound imaging contrast agent, especially organic-inorganic composite lipid microcapsules with uniform particle size as Ultrasound imaging contrast agent has not been reported so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organic-inorganic complex lipid ultrasonography microcapsule and preparation method thereof
  • Organic-inorganic complex lipid ultrasonography microcapsule and preparation method thereof
  • Organic-inorganic complex lipid ultrasonography microcapsule and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] (1) Prepare 0.025M Na respectively 2 CO 3 , Ca(NO 3 )2 100mL Ca(NO 3 ) 2 Add 400mg sulfonated styrene sodium salt (PSS) to the solution, and after it is completely dissolved, quickly add Na 2 CO 3 Solution, stirred at 600rpm for 15s, left standing for 20min, centrifuged and washed three times to obtain calcium carbonate particles, tested by a laser particle size analyzer at a particle size of 5-6 microns, such as figure 2 .

[0047] (2) In negatively charged CaCO 3 The surface is coated with polyallylamine hydrochloride (PAH) through the principle of electrostatic layer-by-layer self-assembly to obtain CaCO 3 -PAH makes its surface positively charged, enhancing the ability to bind with organic-inorganic lipids.

[0048] (3) Precisely weigh 10 mg of organic-inorganic composite lipid 1 and dissolve in 2 mL of chloroform, remove the chloroform by rotary evaporation, and form a transparent lipid film on the bottom of the eggplant-shaped bottle. 4mg / mL uniform part...

Embodiment 2

[0050] Add 200 μL (1 mg / mL) of the organic-inorganic composite lipid microcapsules prepared in Example 1 to a solution of polyethylene glycol octylphenyl ether (TX-100, a surfactant, commonly used to destroy lipids) Medium (TX-100: the molar ratio of organic-inorganic composite lipid microcapsules is 10:1), after standing and blending at room temperature for 30 minutes, centrifuge and wash for 3 to 5 times, then swirl the organic-inorganic composite lipid with pure water Microcapsules. At a suitable concentration, take 10 μL and drop it on the copper grid, dry it at room temperature and test it with a transmission electron microscope, see Figure 5 . It can be seen from the transmission electron microscope photos that the original spherical structure can still be retained and the structure is intact after washing in 10 times the molar ratio of TX-100 solution, while the structure of general liposomes is destroyed after 5 times the TX-100 treatment. This shows that the organi...

Embodiment 3

[0052] After the organic-inorganic composite lipid microcapsules prepared in Example 1 were dried at room temperature, 3 mL of the solution (500 μg / mL) was diluted with physiological saline. Use a 2mL plastic dropper to suck up the organic-inorganic composite lipid microcapsule solution, place it in a water bath at 25°C and place it with an ultrasonic probe for imaging (mechanical index (MI: 0.6, frequency: 2MHz), the test results are as follows: Figure 6 . The experimental results show that organic-inorganic composite lipid microcapsules with hollow structure have obvious ultrasonic signal enhancement effect in clinical ultrasound parameters in vitro ultrasound.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses an organic-inorganic complex lipid ultrasonography microcapsule and a preparation method thereof. The preparation method comprises the following steps: (1) dissolving 5-50mg of organic-inorganic complex lipid in an organic solvent, and carrying out rotary evaporation to remove the organic solvent so as to form a layer of lipid film; (2) adding 5-50mL of a template microsphere water solution into the lipid film for incubation; (3) carrying out water-bath ultrasonic treatment on a mixed solution obtained in the step (2); (4) standing a solution after ultrasonic treatment for 12 hours to 24 hours at room temperature for forming an inosilicate shell layer on the surface of a template microsphere; (5) removing the template microsphere through a physical or chemical method; and (6) carrying out gas or fluorocarbon liquid loading to obtain the organic-inorganic complex lipid ultrasonography microcapsule with a hollow structure. The obtained organic-inorganic complex lipid ultrasonography microcapsule is uniform and controllable in particle size, good in dispersity and high in stability, has a remarkable in-vitro and in-vivo ultrasonography effect, and is expected to have a wide application prospect in the field of disease diagnosis.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to an organic-inorganic composite lipid ultrasonic imaging microcapsule and a preparation method thereof. Background technique [0002] Ultrasound is one of the most important diagnostic tools in clinical practice. Compared with other imaging methods, ultrasound imaging has many advantages such as safety, non-invasiveness, easy operation, real-time imaging, no ionizing radiation, low price, and wide application, so it is one of the most widely used imaging methods in clinical practice. One way to enhance the contrast of ultrasound images is to inject a diagnostic reagent called ultrasound contrast agent into the body through the vein, which can significantly enhance the ultrasound scattering signal, distinguish the different blood perfusion of normal tissue and diseased tissue, and significantly increase the difference between the two. The contrast between images can...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K9/50A61K47/34A61K47/04
Inventor 曹众张春阳蒋庆
Owner SUN YAT SEN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com