A kind of preparation method and application of active low molecular weight fucoidan
A technology of fucoidan and low molecular weight, which is applied in the fields of application, food science, food preservation, etc. It can solve the problems of polysaccharides with large molecular weight and lack of physiological activity, achieve uniform molecular weight, strong controllability, and prevent the shedding of sulfate radicals Effect
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Embodiment 1
[0032] Embodiment 1: the preparation of fucoidan
[0033] 80-mesh kelp powder was added to deionized water at a ratio of 1:25 (w / v), extracted at 80 °C for 3 h, stirred continuously during the extraction process, the stirring speed was 2000 r / min, and centrifuged (3000 r / min, 10 min) , take the supernatant, add HCl (12 mol / L) to make the final concentration 0.1 mol / L; then centrifuge (3000 r / min, 5 min), remove the alginic acid precipitate, collect the supernatant, add absolute ethanol Make the final concentration 30% (v / v), overnight; centrifuge (5000 r / min, 10 min), take the supernatant, and add absolute ethanol to make the final concentration 60% (v / v), Overnight; centrifuge (3000 r / min, 5 min), take the precipitate, and dry it in vacuum to obtain dry fucoidan powder.
[0034] The obtained fucoidan is subjected to infrared spectroscopic analysis, and the results are shown in figure 1 with figure 2 , figure 1 Medium 846 cm -1 The absorption peak at shows that the polys...
Embodiment 2
[0035] Embodiment 2: Preparation of low molecular weight fucoidan
[0036] Hydrolysis: Mix the fucoidan dry powder obtained in Example 1 with β-galactosidase (Shanghai Jiahe Biotechnology Co., Ltd., model: Amresco9031-11-2) and 30000 U / g enzyme activity respectively. g of β-glucosidase (Shanghai Hualan Chemical Technology Co., Ltd., model: 9001-22-3) was mixed evenly at a weight ratio of 1000:0.5:0.5, and deionized water was added to make 3% by weight (w / w) sugar solution, add 4.0 mol / L HCl to adjust the pH value to 5.0, hydrolyze at 37 °C for 5 h, then add 4 mol / L NaOH aqueous solution to adjust the pH value to neutral, heat to 80 °C and keep it warm for 5 min, Thereby, the hydrolysis reaction is terminated, and low-molecular-weight fucoidan aqueous solutions of different molecular fragments are obtained.
[0037] Separation: 100 L of low-molecular-weight fucoidan aqueous solution containing different molecular fragments was separated by a medium-sized ultrafiltration membr...
Embodiment 3
[0039] Embodiment 3: Antibacterial experiment of low-weight fucoidan
[0040] The Staphylococcus aureus (Staphyloccocus aureus) strains involved in this experiment (the strain number is ACCCNO. The preservation date is May 22, 2006) provided by the China Agricultural Microorganism Culture Collection Management Center.
[0041] 1. Experimental method
[0042] a. According to the method of Example 1, extract fucoidan from kelp; according to the method of Example 2, respectively prepare fucoidan with a molecular weight range of ≤5000 Da, 5000-100000 Da and ≥100000 Da, barium sulfate-suspended Nephelometric method detects that sulfate radical content is respectively 28.2 mass %, 26.9 mass % and 25.3 mass %;
[0043] b. Prepare a polysaccharide mother liquor with a concentration of 3.0% by weight of the low-molecular-weight fucoidan prepared in step a, filter and sterilize, and prepare a series of test samples with different concentrations of low-molecular-weight fucoidan with a...
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