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Method for improving brewer's grain bioavailability through combination of mechanical preprocessing and solid-state fermentation

A solid-state fermentation, mechanical method technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of hindering the contact between enzymes and substrates, low bioavailability, and high production costs

Active Publication Date: 2014-02-19
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex structure of brewer's grains, the mutual crosslinking between cellulose and lignin hinders the contact between the enzyme and the substrate, and arabinose, xylose, galactose, etc. replace the xylan backbone in the grain, which leads to enzyme The vitality of the brewer's grains is suppressed, which has a negative impact on the bioavailability of brewer's grains. The low bioavailability leads to high production costs, and there are limiting factors such as market capacity that have not yet achieved large-scale production and application.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] Weigh 50 g of dried brewer's grains, pre-crumble with a hammer mill, then add 500 g of balls, and use a planetary ball mill to process for 6 minutes, controlling the grinding dimension to be between 40-50°C to obtain ball-milled brewer's grains. The particle size distribution was measured by a MICROTRAC laser particle size analyzer, and 95% of the particle size was less than 10 μm. The crude fiber content was determined by sulfuric acid-potassium hydroxide method, and the content was 9.8%, and the crude fiber content of brewer's grains without ball milling was 17.8%. The protein content of brewer's grains after ball milling was 28.8%, the peptide content was 1.2%, the lactic acid content was 0.4%, and the total soluble sugar content was 4.1% as determined by TCA method.

[0012] Take 100g of beer grains crushed by ball milling, add 100g of water, add hemicellulase 200U / g, cellulase 20U / g and protease 200U / g, inoculate with 4% beer yeast (ATCC9763) and 2% pentose Pedioc...

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Abstract

The invention discloses a method for improving brewer's grain bioavailability through combination of mechanical preprocessing and solid-state fermentation. According to the method, a mechanical method is adopted for processing brewer's grains until the grain size reaches the micrometer level, after a cell wall structure is effectively opened, a crude fiber content of the brewer's grains is reduced through biological enzyme processing, and the protein utilization rate is improved; a ratio of grinding balls to the brewer's grains is 10:1, the ball-grinding time is 6 minutes, 200 U / g of hemicellulose, 20 U / g of cellulose and 200 U / g of protease are added to the ground brewer's grains, and 4% of beer yeast and 2% of pediococcus pentosaceus are inoculated and fermented for 48 hours; and finally the materials are dried at the temperature of 60 DEG C and smashed to form a finished product. According to the invention, enzymatic hydrolysate is metabolized by microorganism and converted into beneficial substances, so that the brewer's grain bioavailability is improved, the value in use is effectively improved, and accordingly, one feasible method is provided for improving the additional value of the brewer's grains.

Description

technical field [0001] The invention relates to a method for improving the bioavailability of brewer's grains, in particular to a method for improving the bioavailability of brewer's grains by combining mechanical pretreatment with solid-state fermentation. Background technique [0002] Brewer's grains are the largest by-products in the beer production process. They are the remaining filter residue after malt saccharification, containing malt shell and residual endosperm, rich in cellulose, arabinoxylan, lignin and protein. Although the output of brewer's grains is abundant, the high-value utilization of brewer's grains has not yet been realized at this stage. Most breweries sell them at low prices and use them as feed for ruminants; some breweries directly discharge them as waste, which not only wastes protein resources and have a serious impact on the environment. [0003] Compared with high-quality protein feed fish meal and soybean meal, the protein content of brewer's ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12R1/865
Inventor 蔡国林陆健张麟
Owner JIANGNAN UNIV
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