Method for improving brewer's grain bioavailability through combination of mechanical preprocessing and solid-state fermentation
A solid-state fermentation, mechanical method technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of hindering the contact between enzymes and substrates, low bioavailability, and high production costs
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[0011] Weigh 50 g of dried brewer's grains, pre-crumble with a hammer mill, then add 500 g of balls, and use a planetary ball mill to process for 6 minutes, controlling the grinding dimension to be between 40-50°C to obtain ball-milled brewer's grains. The particle size distribution was measured by a MICROTRAC laser particle size analyzer, and 95% of the particle size was less than 10 μm. The crude fiber content was determined by sulfuric acid-potassium hydroxide method, and the content was 9.8%, and the crude fiber content of brewer's grains without ball milling was 17.8%. The protein content of brewer's grains after ball milling was 28.8%, the peptide content was 1.2%, the lactic acid content was 0.4%, and the total soluble sugar content was 4.1% as determined by TCA method.
[0012] Take 100g of beer grains crushed by ball milling, add 100g of water, add hemicellulase 200U / g, cellulase 20U / g and protease 200U / g, inoculate with 4% beer yeast (ATCC9763) and 2% pentose Pedioc...
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