A method for preparing undenatured natural collagen from suckling pig skin
A technology of natural collagen and skin collagen, applied in the field of preparation of biomedical materials, can solve the problems of lack of collagen fibers, affecting the penetration of chemicals, and destruction of collagen fibers of suckling pig skin, achieving low immunogenicity and ensuring biological activity. Effect
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Embodiment 2
[0043] (1) Raw material pretreatment
[0044] Take 100g of fresh suckling pig skin, remove the subcutaneous fat, and treat it with isopropanol with a material-to-liquid ratio of 1:10 (w / v) and a volume concentration of 45% for 36 hours, accompanied by an intermittent power of 150W ultrasonic wave every 20 minutes and stop for 10 minutes. Repeat 8 times, change the medium 4 times in the middle, rinse with tap water, add the protease NowolaseNG solution with a material-liquid ratio of 1:2.5 (w / v) and a volume concentration of 1.5%, keep the temperature at 35°C, rotate for 45 minutes to remove hair, and then put Cut the skin into pieces, add a 0.3M / L sodium deoxycholate solution with a solid-liquid ratio of 1:30 (w / v), stir for 36 hours, change the solution 4 times in the middle, rinse with tap water, adjust the pH to 8.0, and measure the ash content Content 0.2%. Then use a material-liquid ratio of 1:10 (w / v), volume concentration of 35% acetone and AXAUT-50 composite degreasin...
Embodiment 3
[0051] (1) Raw material pretreatment
[0052] Take 100g of fresh suckling pigskin, remove the subcutaneous fat, and treat it with AXAUT AP with a solid-liquid ratio of 1:5 (w / v) and a volume concentration of 60% for 48 hours, accompanied by intermittent power of 200W ultrasonic waves every 10 minutes and stop for 5 minutes, repeat 10 times, change the liquid 5 times in the middle, rinse with tap water, add protease Basozyme L10 solution with a material-liquid ratio of 1:1.5 (w / v) and a volume concentration of 3%, keep the temperature at 45°C, rotate for 60 minutes to depilate, and then remove the skin Chop up, add a solution of sodium deoxycholate with a ratio of solid to liquid of 1:40 (w / v) and a concentration of 0.05M / L, stir for 48 hours, change the solution 5 times in the middle, rinse with tap water, adjust the pH to 9.0, and measure the ash content 0.2%. Then use a material-liquid ratio of 1:6 (w / v), volume concentration of 50% acetone and AXAUT-50 composite degreasing...
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