A method for hydrophilic modification of polystyrene material surface

A polystyrene and hydrophilic modification technology, which is applied in the field of perfusion chromatographic medium of biological macromolecules, polyvinyl alcohol hydrogel, and surface hydrophilic modification of polystyrene materials, can solve the problem of high protein adsorption and achieve Increased hydrophilicity, reduced non-specific adsorption capacity, and the effect of not falling off easily

Active Publication Date: 2015-10-14
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the amount of protein adsorption is still high compared to agarose separation

Method used

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  • A method for hydrophilic modification of polystyrene material surface
  • A method for hydrophilic modification of polystyrene material surface
  • A method for hydrophilic modification of polystyrene material surface

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Chloroacetylation of polystyrene (PS-Cl)

[0046] Add 0.8g of polystyrene microspheres, 24ml of carbon disulfide, and 1.06g of anhydrous aluminum trichloride into a 50ml flask, stir with a magnet, and the solution turns yellow, then add 0.4ml of chloroacetyl chloride dropwise, and react in an oil bath at 50°C for 3h. The reaction solution was suction-filtered in anhydrous state, the separated solid matter was quickly poured into glacial hydrochloric acid and stirred, filtered again and washed with deionized water until neutral, finally washed and filtered with absolute ethanol, and dried in a vacuum oven.

[0047] The chlorine content of the dried chloroacetylated polystyrene was measured by the sodium hydroxide melting method, and the chlorine content of the modified microspheres was 13.95%. Infrared spectroscopy (FTIR) analysis showed that the chloroacetyl group had been successfully coupled to the surface of the microspheres.

[0048] B Chloroacetylated polystyrene ...

Embodiment 2

[0057] Chloroacetylation of polystyrene

[0058] Add 0.8g of ordinary polystyrene plate, 20ml of dichloromethane, 1.2g of anhydrous aluminum trichloride to a 50ml flask, stir with a magnet, the solution turns yellow, then add 0.8ml of chloroacetyl chloride dropwise, and react in an oil bath at 30°C 5h. The reaction solution was suction-filtered in anhydrous state, the separated solid matter was quickly poured into glacial hydrochloric acid and stirred, filtered again and washed with deionized water until neutral, finally washed and filtered with absolute ethanol, and dried in a vacuum oven.

[0059] B Chloroacetylated polystyrene amination reaction (PS-NH 2 )

[0060] Swell 0.5 g of the above-obtained chloroacetylated polystyrene with 10 ml of chloroform overnight, add 25 ml of an ethanol solution of 0.6 g of hexamethylenetetramine, add 0.64 g of sodium iodide under stirring, and continue mechanical stirring at room temperature for 20 h. Filter and wash thoroughly with etha...

Embodiment 3

[0068] A bromoacetylation reaction of polystyrene

[0069] Add 0.8g of polystyrene microspheres, 24ml of carbon disulfide, and 1.5g of anhydrous aluminum trichloride into a 50ml flask, stir with a magnet, and the solution turns yellow, then add 0.4ml of bromoacetyl bromide dropwise, and react in an oil bath at 50°C for 3h. The reaction solution was suction-filtered in anhydrous state, the separated solid matter was quickly poured into glacial hydrochloric acid and stirred, filtered again and washed with deionized water until neutral, finally washed and filtered with absolute ethanol, and dried in a vacuum oven. The bromine content of the modified microspheres was 25.54%.

[0070] B Chloroacetylated polystyrene amination reaction (PS-NH 2 )

[0071] Swell 0.5 g of the chloroacetylated polystyrene microspheres obtained above with 10 ml of ethanol overnight, add 25 ml of an ethanol solution of 0.45 g of hexamethylenetetramine, add 0.48 g of sodium iodide under stirring, and con...

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Abstract

The invention relates to the field of surface hydrophilic modification of polystyrene material, and especially relates to a surface hydrophilic modification method for a supermacroporous polystyrene microsphere. The method comprises the following steps: first coupling a free radical polymerization initiator, namely 4,4'-azobis(4-cyanovaleric acid), to the surface of a polystyrene material through a three-step chemical reaction, then initiating vinyl acetate and N,N'-methylene diacrylamide to carry out solution polymerization so as to graft a cross-linked polymer layer of polyvinyl acetate onto the surface of the polystyrene material, and finally carrying out an alcoholysis reaction so as to obtain a hydrophilic cross-linked polyvinyl alcohol gel layer. The surface hydrophilic modification method has the advantages of simple operation and mild reaction conditions, the surface hydrophilic performance of the modified polystyrene material is effectively improved, the hydrophilic gel layer is stable, is not easy to fall off, and is rich in hydroxyl groups, so the method has a vast application potential in the field of biological technology, and especially in the field of chromatogram separation.

Description

technical field [0001] The invention relates to a method for hydrophilic modification of the surface of a polystyrene (PS) material, especially for the hydrophilic modification of the surface of super-large-porous PS microspheres, which can be used on the surface of super-large-porous PS microspheres (including the inner surface of the channel) ) to polymerize a layer of hydroxyl-rich polyvinyl alcohol (PVA) hydrogel, which is further derivatized as a perfusion chromatographic medium for the rapid separation of biomacromolecules such as proteins. The invention belongs to the field of functional modification of polymer microspheres. Background technique [0002] Organic polymer materials are widely used in textile, construction, biomedicine and other fields, and are important materials in science and technology and economic construction. In some fields, the hydrophilicity of materials is very high in practical applications. In the textile industry, due to the hydrophobicity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F257/02C08F218/08C08F222/38C08F8/18C08F8/24C08F8/32C08F8/10C08F8/00
Inventor 曲剑波王晓娟陈艳丽宦关生朱虎
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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