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Construction method and applications of high performance liquid chromatography (HPLC) fingerprint of alkaloid compounds of dendrobe

A technology of high-performance liquid chromatography and dendrobium alkaloids, applied in the direction of material separation, analysis materials, measuring devices, etc., can solve the problems of inability to accurately identify and evaluate the quality of dendrobium alkaloid components, lack of research and comparison of dendrobium alkaloids, and achieve Good peak shape, great stability and reproducibility, and high separation efficiency

Inactive Publication Date: 2014-02-26
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

At present, in the literature published at home and abroad, only microscopic identification, thin-layer chromatography, high-performance liquid chromatography, gas chromatography and mass spectrometry are used to study some characteristic components in a certain Dendrobium, and there is a lack of overall alkaloids in different Dendrobium species. Systematic research and comparison, it is obvious that this quality evaluation method cannot accurately identify and evaluate the quality of Dendrobium alkaloid components

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  • Construction method and applications of high performance liquid chromatography (HPLC) fingerprint of alkaloid compounds of dendrobe
  • Construction method and applications of high performance liquid chromatography (HPLC) fingerprint of alkaloid compounds of dendrobe
  • Construction method and applications of high performance liquid chromatography (HPLC) fingerprint of alkaloid compounds of dendrobe

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Embodiment Construction

[0022] The technical solution of the present invention is further illustrated and described below through specific embodiments

[0023] (1) Material collection

[0024] Dendrobium officinale, Dendrobium copper, and Dendrobium Huoshanense were used as test materials; tissue culture of Dendrobium officinale, Dendrobium copper, and Dendrobium Huoshanense was carried out by conventional methods. The tissue culture conditions were: 25°C±2°C, 12h light and 12h dark, after 5 times of subculture, the subculture period is about 45 days, and the tissue culture seedling 1, the tissue culture seedling 2, and the tissue culture seedling 3 are obtained after culture.

[0025] (2) Preparation of the test solution: Take a few fresh seedlings of Dendrobium, kill them in an oven at 105°C for 15-20 minutes, dry them in an oven at 60°C, crush them, and pass them through a 60-mesh sieve. Take 0.5g dry powder of several samples, add 5ml ammonia water respectively, rinse the plug for 30min, add 50m...

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Abstract

The invention discloses a construction method of fingerprint of alkaloid compounds of dendrobe. According to the construction method, thermostatic waterbath reflux extraction technology is adopted; dendrobe powder is immersed in ammonium hydroxide, and is subjected to extraction with chloroform, filtration, condensation and drying so as to obtain a dendrobe total alkaloid crude product; the dendrobe total alkaloid crude product is dissolved in methanol so as to obtain a stock solution; and the stock solution is used for high performance liquid chromatographic analysis so as to obtain the dendrobe alkaloid compound fingerprint. Advantages of the construction method are that: the obtained chromatographic fingerprint is capable of comprehensively reflecting alkaloid compounds contained by dendrobium, reflecting complexity of dendrobium alkaloid compounds, and providing scientific basis for identification of different dendrobium varieties; the constructed HPLC fingerprint possesses relatively high stability and repeatability; and scientific reference is provided for quality control of dendrobium variety.

Description

technical field [0001] The invention relates to a method for constructing high-performance liquid chromatography fingerprints of dendrobium alkaloid components, and belongs to the technical field of biological products and traditional Chinese medicine preparation. Background technique [0002] Alkaloids are one of the important medicinal components in Dendrobium, and they are also the earliest compounds isolated and confirmed in their structures in Dendrobium. It is generally believed that the pharmacological effects of Dendrobium alkaloids are mainly manifested in anti-tumor, cardiovascular and gastrointestinal inhibition, pain relief, fever reduction and strengthening the mind. Studies have shown that Dendrobium, the main component of Dendrobium nobile, has analgesic and antipyretic effects, can lower heart rate, blood pressure, slow down breathing, can produce moderate hyperglycemia, has a strong effect and can relieve barbiturate poisoning. However, the composition and ...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 林毅宋程金青蔡永萍钟伦锁孙时伟张玲
Owner ANHUI AGRICULTURAL UNIVERSITY
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