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Structured light illumination microscopic imaging system based on silicon-based liquid crystal chip

A technology of structured light illumination and liquid crystal on silicon, which is applied in the field of imaging systems, can solve problems such as reducing system stability, and achieve the effects of avoiding impact, realizing portable design, and reducing system volume.

Inactive Publication Date: 2014-02-26
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In these systems that use gratings to obtain structured light illumination, the gratings must be moved to obtain source images at different phase shifts, so this mechanical movement reduces the stability of the system

Method used

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  • Structured light illumination microscopic imaging system based on silicon-based liquid crystal chip
  • Structured light illumination microscopic imaging system based on silicon-based liquid crystal chip
  • Structured light illumination microscopic imaging system based on silicon-based liquid crystal chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Such as figure 1 As shown in the figure, the working principle diagram of the structured illumination micro-imaging system using gratings in the current structure. The optical path is mainly composed of grating, illumination source, lens system, CCD and synchronous control system. The light emitted by the light source is collimated, and the structured light is generated by the grating to illuminate the surface of the object to be measured. The lens system images the fluorescence emitted by the object surface on the CCD plane. The piezoelectric device is used to synchronize the movement of the grating (corresponding to the phase shift) with the speed of the CCD recording image, that is, after an image is recorded, the grating moves a displacement to record the image corresponding to the new bit. The recorded images are reconstructed using a specific algorithm to obtain tomographic images and ultra-high-resolution two-dimensional images. This type of optical path has si...

Embodiment 2

[0035] Such as figure 2 As shown, the structured light illumination super-resolution microscopy system using the liquid crystal-on-silicon chip LCoS development system 9 of the present invention, the light emitted from the high-brightness LED light source 1 is first collimated by the collimation module 2, and then enters the polarizing beam splitter In PBS3, the P-polarized light is transmitted and incident to the liquid crystal on silicon LCoS chip 901 , while the S-polarized light is reflected and cannot enter the optical path. The liquid crystal on silicon chip LCoS901 is controlled by the development system, and the target structure pattern is loaded to each pixel of the LCoS, so the incident S-polarized light is modulated, and when the pixel is in the "on" state of "1", the light passes through the pixel The polarization direction is rotated by 90 degrees, so as to become the P polarization state, which is reflected by the polarization beam splitter PBS3 and enters the o...

Embodiment 3

[0038] Such as image 3 As shown, a high-brightness LED module includes a high thermal conductivity substrate 1 and LED chips 2, the LED chips 2 are 2 LED chips with 9 groups of different central wavelengths, and the number of each group of LED chips 2 is at least one , the LED chip 2 is a common anode structure; the LED chip 2 is directly fixedly connected to the high thermal conductivity substrate 1 .

[0039] The central wavelength ranges of the nine LED chips 2 cover the ultraviolet band to the infrared band, and are individually controlled by independent control circuits. The control circuit includes a switch circuit that can control the circuit on and off of the corresponding group of LED chips, and also includes a current control circuit that controls the magnitude of the energizing current of the group of LED chips. Under the control of the driving circuit, single or multiple LED chips realize light emission.

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Abstract

The invention discloses a structured light illumination microscopic imaging system based on a silicon-based liquid crystal chip. The system comprises a high brightness LED excitation light source. A collimating module is arranged in front of the high brightness LED excitation light source. A polarization beam splitter (PBS) is arranged in front of the collimating module. A silicon-based liquid crystal chip LCoS development system is arranged in front of the polarization beam splitter (PBS). The silicon-based liquid crystal chip LCoS development system is connected with a computer which is connected with a photoelectric detector. A narrowband filter is arranged in front of the photoelectric detector. A focusing object lens is arranged in front of the narrowband filter. A dichroic mirror is arranged in front of the focusing object lens. A microscope object lens is arranged in front of the dichroic mirror. An objected to be measured is arranged in front of the microscope object lens. The technical scheme of the invention has the advantages of high utilization rate of light energy, a small system size, an adjustable structured light illumination cycle and high imaging resolution and is more suitable for the real-time 3D imaging research of a living biological cell.

Description

technical field [0001] The invention relates to an imaging system of a super-resolution microscope, in particular to a structured light microscopy imaging system based on a silicon-based liquid crystal chip. Background technique [0002] At present, high-resolution optical microscopy has become an important tool for original innovation and major breakthroughs in the fields of biology, medicine, and pharmacy. , The development of live real-time imaging puts forward higher requirements. [0003] Ordinary optical microscopes are limited by the optical diffraction limit. The lateral resolution can only reach 200nm, and the vertical resolution is about 500nm. It is difficult to meet the research needs of subcellular and molecular structures in the current biological field. [0004] Structured light imaging technology was first proposed by Neil et al. in 1997, and applied to biological imaging, the resolution can reach 100nm. This technology uses a specially modulated structured...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/06G02B21/36
Inventor 熊大曦杨西斌朱剑锋李辉
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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