Method for preparing L-lactic acid by using lemna mior raw material
A lactic acid, duckweed technology, applied in the field of environmental biotechnology and biomass energy, can solve the problems such as not appearing
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[0020] Preparation of duckweed starch saccharification solution: Duckweed was dried and crushed, passed through a 60-mesh sieve, prepared into a suspension with a concentration of mass volume ratio of 1:9 using a phosphate buffer solution with a pH value of 6.5, and firstly washed with a medium-temperature α- Amylase degrades starch granules and prepares starch enzymolysis solution. In order to ensure the effect of enzymolysis, CaCl with a mass volume concentration of 0.2% is added to the prepared material 2 , raise the temperature to 60°C to liquefy until the test result of the iodine solution is brown orange, then heat and boil for 10 minutes to inactivate the enzyme activity; after the liquefied solution is cooled, adjust the pH value to 4.5 with dilute hydrochloric acid, and then continue to add glucoamylase Make it fully degraded, and inactivate the enzyme activity at 100°C after the saccharification is completed, so as to prepare the saccharification liquid required for f...
Embodiment 1
[0029] Take 8g duckweed powder (starch content 30%, protein content 15%), 4.8g CaCO 3 Put 47.2ml of distilled water in a 250ml Erlenmeyer flask, saccharify it with medium-temperature α-amylase and glucoamylase, sterilize at 115°C for 20 minutes, and then inoculate the seed solution with an inoculum of 6% (V:V) Inoculated into the fermentation medium, after static fermentation at 37°C and 150r / min for 48 hours, the content of calcium lactate was determined by EDTA titration, and finally lactic acid was separated and purified by ion exchange method and quantified.
Embodiment 2
[0031] Take 8g of duckweed powder (starch content 30%, protein content 15%), yeast powder 1.2g and place in a 250ml Erlenmeyer flask, adjust the pH value to 6.3 with hydrochloric acid and sodium hydroxide, add 46ml of distilled water, and prepare CaCl with a mass volume concentration of 0.2% is added to the material 2 In order to ensure the effect of enzymatic hydrolysis, after saccharification with medium-temperature α-amylase and glucoamylase, sterilize at 115°C for 30 minutes, and finally add CaCO sterilized by dry heat 3 4.8g, then inoculate the seed solution into the fermentation medium at an inoculum size of 8% (V:V), and after static fermentation for 72 hours at 37°C and 150r / min, use the EDTA titration method to measure the calcium lactate content, and finally Lactic acid was isolated and purified by ion exchange and quantified.
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