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A kind of cassava residue anaerobic fermentation residue producing cellulase and its saccharification method

An anaerobic fermentation and cellulase technology, applied in the field of microorganisms, can solve the problems of cellulose energy consumption and high cost, high cost, poor cellulase activity, etc., and achieve the goal of reducing environmental pressure, creating economic value, and process economy Effect

Active Publication Date: 2016-05-04
临泉县嘉鸿装饰工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, cellulose pretreatment technologies such as physical methods, chemical methods, physicochemical methods, and biological methods generally have problems such as high energy consumption, high cost, low efficiency, and difficulty in scaling up.
Among them, although the dilute acid method has been scaled up, the yield is low, and the harmful by-products in the product affect further biotransformation and utilization.
Biological method is environmentally friendly, with low energy consumption and few by-products, but low conversion efficiency, poor cellulase activity and high cost
It is difficult to efficiently convert cellulose into sugar compounds by current conventional methods, which has become a bottleneck restricting the efficient conversion and utilization of cellulose
The cost of the steam explosion pretreatment process is low, but the treatment quality is not stable

Method used

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  • A kind of cassava residue anaerobic fermentation residue producing cellulase and its saccharification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Production of Geotrichum Galactomycessp.CCZU11-1 Cellulase by Fermentation

[0025] 3 L medium (NH 4 Cl5g / L, KH 2 PO 4 2g / L, MgSO 4 0.3g / L, CaCl 2 0.6g / L, polyethylene glycol 60004g / L, cassava residue anaerobic fermentation residue 10g / L, pH6.5), culture Galactomycesssp .CCZU11-13d. The filter paper enzyme activity (FPA), endonuclease activity (CMCase) and total protein content of the obtained cellulase fermentation broth were 29.3U / mL, 59.9U / mL and 1.6g / L, respectively.

Embodiment 2

[0026] Example 2 Phosphoric acid with a final mass concentration of 0.5% assists 1-ethyl-3-methyl-imidazole-diethylphosphate ([Emim][DEP]) to pretreat cassava residue anaerobic fermentation residue

[0027] In the pretreatment system, 85% (w / w) of 1-ethyl-3-methyl-imidazole-diethylphosphate ([Emim][DEP]), 0.5 % (w / w) phosphoric acid and 14.5% (w / w) water mixture was dissolved and pretreated at 130°C for 30 minutes, then deionized water was added to wash and regenerate to obtain regenerated cellulose raw materials. After the regenerated cellulose material was washed three times with deionized water, 80mL of acetic acid-sodium acetate buffer solution (50mM, pH4.8) and 20mL of cellulase fermentation broth were added to form a 100mL mixed system. The concentration is 3%. The reaction was shaken on a constant temperature shaker at 50°C and 180rpm for 72h, and the yield of reducing sugar was 40.5%.

Embodiment 3

[0028] Example 3 Phosphoric acid with a final mass concentration of 5% assists 1-ethyl-3-methyl-imidazole-diethylphosphate ([Emim][DEP]) to pretreat cassava residue anaerobic fermentation residue

[0029] In the pretreatment system, 3g of dried cassava residues from anaerobic fermentation utilized 70% (w / w) of 1-ethyl-3-methyl-imidazole-diethylphosphate ([Emim][DEP]), 5 % (w / w) of phosphoric acid, 25% (w / w) of water mixture was dissolved at 110 ° C for 120 minutes, then added deionized water to wash and regenerate; the regenerated cellulose material was washed three times with deionized water, then added 80mL of acetic acid-sodium acetate buffer solution (50mM, pH4.8) and 20mL of cellulase fermentation broth form a 100mL mixed system, and the final mass concentration of cellulose raw material from anaerobic fermentation of cassava residues is 3%. The reaction was shaken on a constant temperature shaker at 50°C and 180rpm for 72h, and the yield of reducing sugar was 42.4%. Add...

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Abstract

The invention relates to a method for producing cellulase by cassava dreg anaerobic fermentation residues and a saccharification method of the cellulose, and belongs to the field of microbial technology. The invention relates to a method for producing high-activity Galactomycessp. CCZU11-1 cellulase by using cassava dreg anaerobic fermentation residues as geotrichum carbon sources, which makes high-efficient use of cassava dreg anaerobic fermentation residues. The produced cellulase can realize high-efficient saccharification of cassava dreg anaerobic fermentation residues pretreated by a 1-ethyl-1-methyl-imidazole-diethyl phosphate ([Emim][DEP])-phosphoric acid-water system, and has a total saccharification rate of more than 40%. The method of the invention is simple in process, and has the advantages of high efficiency, economical process, and few 'three wastes'.

Description

technical field [0001] The invention discloses a method for producing Geotrichum galactomycesssp. vitrinase and its saccharification by using the anaerobic fermentation residue of cassava dregs as a carbon source, and belongs to the technical field of microorganisms. Background technique [0002] The solid waste produced during the deep processing of cassava starch - cassava residue, its dry base contains rich woody crude cellulose, a small amount of starch and protein. Crude fiber has high stability, is not easy to decompose, and is difficult to handle. According to statistics, for every 1 million tons of cassava raw materials processed, about 200,000 tons of cassava residue waste will be generated. If the waste is directly discharged or incinerated, it will cause environmental pollution and a huge waste of lignocellulosic resources. The anaerobic fermentation of cassava residues to produce biogas is an important way, but the biogas residues still contain a lot of lignoce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12P19/14C12P19/02
CPCC12N9/2437
Inventor 何玉财龚磊李创陶志成王茜张跃陆婷吴殷琦张丹平
Owner 临泉县嘉鸿装饰工程有限公司
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