Half-path charge complementary type chiral self-assembled short-peptide nanometer biomedical material and application

A self-assembled short peptide, complementary technology, applied in the fields of medical science, peptides, absorbent pads, etc., can solve problems such as instability, achieve the effects of secondary structure stabilization, prolonged action time, and rapid hemostasis wound healing

Inactive Publication Date: 2014-03-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a small number of short peptides with hemostatic activity have been reported at home and abroad, but they are l...

Method used

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  • Half-path charge complementary type chiral self-assembled short-peptide nanometer biomedical material and application
  • Half-path charge complementary type chiral self-assembled short-peptide nanometer biomedical material and application
  • Half-path charge complementary type chiral self-assembled short-peptide nanometer biomedical material and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] When X is Glu and Y is Arg, the sequence of half-range charge complementary chiral short peptide ①D-P9-1 is as follows:

[0039] D-P9-1: Ac-Pro D -Glu D -Phe D -Arg D -Phe D -Asn D -Phe D -Gln D -Pro D -NH 2 . Half-range charge-complementary chiral short peptide ①Synthesis of D-P9-1: Ac is the acetylated N-terminal, NH 2 For amidated C-terminus

[0040] 1. Materials

[0041] Fmoc-Pro-OH(N-fluorenylmethoxycarbonyl-proline), Fmoc-Glu(OtBu)-OH(N-fluorenylmethoxycarbonyl-o-tert-butyl-glutamic acid), Fmoc-Phe-OH (N-fluorenylmethoxycarbonyl-phenylalanine), Fmoc-Arg(pbf)-OH(N-fluorenylmethoxycarbonyl-2,2,4,6,7-pentamethyldihydrobenzofuran- 5-sulfonyl-arginine), Fmoc-Asn(Trt)-OH (N-fluorenylmethoxycarbonyl-N-trityl-asparagine), Fmoc-Gln-OH (N-fluorenylmethoxycarbonyl Acyl-Glutamine), Rink Amide-MBHA Resin.

[0042] HBTU (O-benzotriazol-1-yl-N, N, N, N-tetramethylurine hexafluorophosphate) and HOBT (1-hydroxybenzotriazole) were purchased from Shanghai Keyept Bioch...

Embodiment 2

[0058] High-performance liquid chromatography and mass spectrometry detection and three-dimensional molecular model drawing of the half-range charge-complementary chiral self-assembled short peptide ①D-P9-1 of the present invention

[0059] The half-course charge-complementary chiral self-assembled short peptide ①D-P9-1 prepared in Example 1 was detected by high performance liquid chromatography, and the detection results are shown in figure 1 ,according to figure 1 The results in determined that its purity reached 95.5%.

[0060] The half-range charge-complementary chiral self-assembled short peptide ①D-P9-1 prepared in Example 1 was detected by mass spectrometry, and the detection results are shown in figure 2 , the result showed that its molecular weight was 1222.38.

[0061] The half-range charge complementary chiral self-assembled short peptide ①D-P9-1 prepared in Example 1 was used to draw a schematic diagram of a three-dimensional molecular model based on the princip...

Embodiment 3

[0063] Detecting the secondary structure of the half-range charge-complementary chiral self-assembled short peptide ① D-P9-1 of the present invention using a circular dichroism spectrometer

[0064] 1. Detection of half-range charge-complementary chiral self-assembled short peptides using circular dichroism ① Secondary structure changes of D-P9-1 in solutions with different peptide concentrations

[0065] Test sample preparation: Take the half-range charge-complementary chiral self-assembled short peptide ①D-P9-1 stored at 4°C and prepare it with Milli-Q ultrapure water to the concentrations: 0.05mg / ml, 0.1mg / ml, 0.2 mg / ml, 0.5 mg / ml and 1.0 mg / ml test sample solutions.

[0066] Experimental operation and instrument parameter setting: Applied photophysics CD spectrometer is used for CD spectrum. A 1mm quartz cuvette is used during the experiment. Before the test, a small amount of test sample should be used to rinse the quartz cuvette to reduce experimental errors. Accurately ...

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Abstract

The invention discloses a half-path charge complementary type chiral self-assembled short-peptide nanometer biomedical material and application. The general formula of the amino acid sequence of a chiral self-assembled short peptide provided in the invention is shown as follows: Ac-Pro-X-Phe-Y-Phe-Asn-Phe-Gln-Pro-NH2, wherein X and Y are polar charged amino acids, X is an negatively charged amino acid and Y is an positively charged amino acid, the amino acids of the whole peptide chain are all D-amino acid, and the number of amino acid residues is set as 9. The half-path charge complementary type chiral self-assembled short peptide has a secondary structure with relatively strong stability and is hardly influenced by external environmental factors, the self-assembly morphology of the chiral short peptide can be regulated by adding NaCl or changing the content of NaCl, and nanometer fiber hydrogel, which has rapid bleeding-stopping effect on bleeding wound, can be formed through self assembly. The material is simple and practicable in preparation technology and relatively low in cost, and the application of the material helps to enrich the kinds of nanometer biomedical materials.

Description

technical field [0001] The invention relates to the field of self-assembled nano biomedical materials, in particular to a class of half-course charge-complementary chiral self-assembled short peptides and their application in rapid hemostasis. Background technique [0002] Due to the fact that the phenomenon of self-assembly exists widely in nature, the design of self-assembled peptide nanomaterials using amino acid residue sequences has gradually become a hot research field in recent years. By rationally designing the amino acid residue sequence of the polypeptide chain, polypeptide molecules can use non-covalent bonding forces such as electrostatic interaction, hydrophobic interaction, hydrogen bond interaction, and π-π stacking interaction to spontaneously or trigger self-assemble to form specific structures and shapes. self-assembly. Since D-amino acids have good biocompatibility and are not easy to be degraded by naturally occurring amino acid decomposing enzymes, the ...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61L15/32A61L15/44
Inventor 何道航邓淑晶周立新
Owner SOUTH CHINA UNIV OF TECH
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