Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium

A technology of sulfur-oxidizing bacteria and thermophilic Thiobacillus, applied in the direction of microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of low yield of elemental sulfur and low desulfurization efficiency, and achieve an enhanced system Effects of mixing, high-efficiency oxidation, and high-efficiency elemental sulfur production capacity

Active Publication Date: 2014-03-26
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there have been reports of bacterial strains and various types of reactors applied to sulfide removal in Chi

Method used

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  • Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium
  • Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium
  • Sulfur-oxidizing bacterium and method for removing sulfide by using sulfur-oxidizing bacterium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 strain screening

[0026] In this example, the sludge from an anaerobic pond of a sewage treatment plant in Jiangsu Province was taken, and an inoculation amount of 5% was added to a 250mL triangular flask containing 100mL thiosulfate medium, pH=7, 37°C, 170r / min, Carry out enrichment culture for 4 days, repeat 3-4 times, use sodium thiosulfate medium for plate separation, after 3 days, colonies are formed, pick a single colony and insert it into sodium thiosulfate medium for culture, repeat plate separation 4 times to obtain pure bacteria.

Embodiment 2

[0027] Embodiment 2 strain identification

[0028] The whole genome of the pure culture was extracted using the bacterial whole genome rapid extraction kit, and PCR was performed with 16S rDNA universal primers to identify the strain as Thiobacillus thermophiles ( Themithiobacillus tepidarius ).

Embodiment 3

[0029] Example 3 Physiological, biochemical and batch biological desulfurization of bacterial strains

[0030] The morphology of the above-mentioned JNU-2 was observed by transmission electron microscopy as follows: figure 1. Take the strains screened at initial pH = 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 for cultivation, and determine the optimum pH for bacterial growth to be 8.0, and the optimum pH for elemental sulfur production to be 6.0; , 40°C, 43°C, 46°C and 50°C were cultivated at the optimum pH for elemental sulfur production, and the optimum temperature range for growth and elemental sulfur production was determined to be 37-43°C. Determine the optimal energy substrate (g / L) for strain JNU-2 by the following combinations: yeast extract 1.0; peptone 1.0; glucose 1.0; fructose 1.0; sucrose 1.0; lactose 1.0; galactose 1.0; maltose 1.0; glycine 1.0 ; Lysine 1.0; Methionine 1.0; Na 2 S 2 o 3 ·5H 2 O 10.0; Na 2 S 1.0; S 0 1.0; yeast extract 1.0 and Na 2 S 2 o 3 ...

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Abstract

The invention discloses a sulfur-oxidizing bacterium and a method for removing sulfide by using the sulfur-oxidizing bacterium and belongs to the technical field of biological and environmental protection. The invention discloses the bacterium classified and named Themithiobacillustepidarius JUN-2, and the preservation number is CCTCCNO:M2013217. The bacterium has very high oxidizing efficiency for sulfur compounds, and a byproduct named elemental sulfur with high additional value is efficiently generated. The invention further relates to a method for removing the sulfur compounds in sulfur-containing waste liquid by applying the the sulfur compounds in sulfur-containing waste liquid in a gas lifting type inner circulation continuous operation aerobic reaction device; the hydraulic retention time (HRT) is 10 hours; the yield of the elemental sulfur is 0.1-gL<-1>h<-1>; the generation ratio of the elemental sulfur is about 60 percent; the oxygenation efficiency of thiosulfate is greater than 98 percent. The gas lifting type inner circulation continuous operation aerobic reaction device behaves well, and has a potential to removal of the sulfides in the sulfur-containing waste liquid on large scale.

Description

technical field [0001] The invention relates to a strain of mesothermophilic Thiobacillus and a method for removing sulfide by applying the strain to an air-lift internal circulation continuous operation aerobic reaction device, which can be applied to biogas, natural gas desulfurization, and sulfate-containing wastewater and smoke Dao gas SO 2 The biological removal of sulfide produced by the absorption liquid through biological anaerobic reduction. It belongs to the technical field of biology and environmental protection. Background technique [0002] Biological desulfurization is an interdisciplinary subject in the field of biology and environmental protection. It is also called biocatalytic desulfurization. It uses microorganisms or the enzymes it contains to catalyze sulfur-containing compounds (sulfides, organic sulfur) to release the sulfur contained in it ( The process of converting into elemental sulfur or sulfate); compared with ordinary desulfurization methods, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 杨海麟高凯王武辛瑜夏小乐张玲
Owner JIANGNAN UNIV
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