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Method for detecting erythritol

A technology of erythritol and detection method, which is applied in the field of chemical analysis, can solve the problems of complicated sample preparation and low sensitivity of differential refractive index detector, and achieve the effects of environmental friendliness, high sensitivity and high accuracy

Inactive Publication Date: 2014-03-26
BEIJING GUANHONG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When using HPLC to measure sugar alcohols, since they have no absorption and no fluorescence in the ultraviolet region or visible light range, sugar alcohols are mostly detected by differential refractive index detectors, but the differential refractive index detectors have low sensitivity and the quantitative level is only 10. -1 g / L order of magnitude
The sample component is prepared into a substance with ultraviolet absorption or fluorescence by derivatization method. Using ultraviolet, diode array or fluorescence detector, the analysis sensitivity can be greatly improved, and the quantitative level can reach 10 -2 On the order of g / L, but the sample preparation operation is complicated, and it is necessary to select a suitable derivatization reagent

Method used

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  • Method for detecting erythritol

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Experimental program
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Effect test

Embodiment 1

[0015] The present invention selects ion chromatography and anion exchange column to carry out the chromatographic analysis of sugar alcohol, when its pH value is greater than the pKa value of sugar alcohol in strong alkaline solution, sugar alcohol will be partially or completely dissociated, and exists in the solution with anion form, In view of the pKa value of sugar alcohols in the range of 12 to 14, a larger concentration of sodium hydroxide was used as the eluent for anion exchange separation, and its concentration was determined by experiment.

[0016] The chromatographic conditions are: sodium hydroxide is as mobile phase, 30 ℃ of column temperatures, pulsed amperometric detector, and flow rate is 0.4ml / min, and chromatographic column is DIONEX Carbopac PA10 anion analysis column (4mm * 250mm), DIONEX Carbopac PA10 anion protection column ( 4mm×50mm). Mobile phase concentration a is 1000mmol / L, concentration b is 750mmol / L, concentration c is 500mmol / L, concentration d...

Embodiment 2

[0019] The preliminary chromatographic conditions are: 500mmol / L sodium hydroxide as mobile equipotential elution, column temperature 30°C, pulse amperometric detector (gold electrode), chromatographic column is DIONEX Carbopac PA10 anion analysis column (4mm × 250mm), DIONEX Carbopac PA10 Anion guard column (4mm×50mm). Fix the above conditions and compare the influence of different mobile phase flow rates on the analysis results. The flow rate a is 0.2ml / min, the flow rate b is 0.3ml / min, the flow rate c is 0.4ml / min, and the flow rate d is 0.5ml / min. Since the Carbopac PA10 chromatographic column has a flow rate limitation, it is considered to compare the ion chromatography analysis results of erythritol under the four flow rates of a, b, c, and d.

[0020]When the mobile phase flow rate is 0.2ml / min, the retention time of erythritol is about 21min; when the mobile phase flow rate is 0.3ml / min, the retention time of erythritol is about 14min; when the mobile phase flow rate ...

Embodiment 3

[0022] It was found that the retention of sugar alcohols in anion-exchange chromatography is an exothermic process (retention decreases with increasing temperature). However, each sugar alcohol is affected to a different degree by temperature. Therefore, the above chromatographic conditions were fixed, and the analysis results of four different column temperatures of 20°C, 30°C, 35°C, and 40°C were compared. Comparing the chromatographic analysis results corresponding to four different column temperatures of 20°C, 30°C, 35°C, and 40°C, it can be seen that the retention time increases with the decrease of the column temperature. Then, there is also a phenomenon that when the column temperature is 30°C, the chromatographic peaks of erythritol and impurities do not reach the baseline separation, and when the column temperature is 35°C, the chromatographic peaks of erythritol and impurities are completely separated, therefore, The selected column temperature is 35°C.

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Abstract

Erythritol is a white crystalline tetra-carbon polyol compound and a novel fermentation type low-calorie sweetener, and has wide application prospect in the fields such as medicine, food, cosmetics, and chemical industry; the study on the erythritol becomes a high-profile hot spot internationally at present. The invention provides a method for quantitative detection of the erythritol by ion chromatography; due to the method, the recovery rate reaches 85-90%; compared with the existing gas chromatographic method, a high performance liquid chromatography, a capillary electrophoresis method, the method has the advantages of high accuracy, no derivation, simplicity and convenience in operate, high sensitivity, environmental protection, good reproducibility and the like, thus being a practical detection method.

Description

1. Technical field [0001] The invention belongs to the field of chemical analysis, in particular to the technology of detecting erythritol by ion chromatography. 2. Background technology [0002] The chemical name of erythritol is 1,2,3,4-butanetetraol, the English name is Erythritol, and the molecular formula is C 4 h 10 o 4 , the molecular weight is 122.12, and the chemical structural formula is as follows figure 1 As shown, it has a melting point of 126°C and a boiling point of 329-331°C. It is a four-carbon polyol with a white powdery crystal appearance, cool feeling, low calorific value and easy crystallization. [0003] Erythritol is a white crystalline four-carbon polyol compound. It is a new type of fermented low-calorie sweetener produced by biotechnology. In June 1999, the International Committee of Experts on Food Additives (JECFA) approved erythritol as a Edible sweeteners, and there is no need to specify the ADI value. At present, erythritol has been used i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 许波贺玉荣
Owner BEIJING GUANHONG TECH