Ganoderma lucidum-ginkgo functional food and preparation method thereof
A technology of functional food and ginkgo, applied in the field of bioengineering, can solve problems such as high cost, heavy pollution, and environmental pollution
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Embodiment 1
[0016] Preparation of strains in liquid shake flasks: 20 grams of sucrose, 50 grams of soybean cake powder, 50 grams of corn steep liquor, 60 grams of bran, 0.6 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 40mL bottle, sterilized at 100°C for 60 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganoderma lucidum ), cultured at 22°C for 10 days at a rotating speed of 60 rpm, to obtain strains of Ganoderma lucidum liquid shake flasks;
[0017] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.6 grams of potassium dihydrogen phosphate, and 0.5 grams of magnesium sulfate according to the volume of the seed tank , 7 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transfe...
Embodiment 2
[0021]Preparation of strains in liquid shake flasks: 60 grams of sucrose, 30 grams of soybean cake powder, 30 grams of corn steep liquor, 30 grams of bran, 0.3 grams of potassium dihydrogen phosphate, 0.3 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 80mL bottle, sterilized at 115°C for 40 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganoderma lucidum ), cultured at 120 rpm at a temperature of 28°C for 7 days to obtain a strain of Ganoderma lucidum liquid shake flask;
[0022] Preparation of liquid expanded culture strains: according to the volume of the seed tank, weigh 60 grams of sucrose, 25 grams of soybean cake powder, 31 grams of corn steep liquor, 30 grams of bran, 0.25 grams of potassium dihydrogen phosphate, and 0.25 grams of magnesium sulfate according to the volume of the seed tank , 4.1 grams of soybean oil, 1000 mL of water; the liquid submerged culture medium was transferred to t...
Embodiment 3
[0025] Preparation of strains in liquid shake flasks: 100 grams of sucrose, 10 grams of soybean cake powder, 10 grams of corn steep liquor, 10 grams of bran, 0.06 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 1000 mL of water, divided into 250 mL triangular flasks, each 140mL bottle, sterilized at 128°C for 55 minutes, cooled and then inserted into the slant of the ganoderma test tube ( Ganoderma lucidum ), cultivated at 180 rpm at a temperature of 33°C for 5 days to obtain the ganoderma lucidum liquid shake flask strain;
[0026] ` Preparation of liquid expanded culture strains: According to the volume of the seed tank, weigh 20 grams of sucrose, 45 grams of soybean cake powder, 45 grams of corn steep liquor, 60 grams of bran, 0.06 grams of potassium dihydrogen phosphate, and 0.05 grams of magnesium sulfate according to the volume of the seed tank. gram, soybean oil 2 grams, water 1000mL; the liquid submerged culture medium is transferred t...
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