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A method for increasing the yield of l-glutamine

A technology of glutamine and seeds, which is applied in the field of extraction and refining of amino acid derivative fermentation liquid, can solve problems such as low production capacity and output value, pollution, etc., and achieve the effects of reducing dosage, reducing production cost, and improving purity

Inactive Publication Date: 2016-04-06
SHANDONG MINQIANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because L-glutamine not only has important scientific research, practical production and application value, but also plays an important role in physiological functions, and the traditional domestic production methods are still seriously polluted and the production capacity is low. Therefore, Approved by the State Council and representatives of the National People's Congress, the production of L-glutamine by fermentation has been included in the national "Eleventh Five-Year Plan" key scientific and technological projects

Method used

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  • A method for increasing the yield of l-glutamine
  • A method for increasing the yield of l-glutamine
  • A method for increasing the yield of l-glutamine

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preparation example Construction

[0059] (1) Preparation of fermentation broth:

[0060] (1) Slant culture: insert the strains of the amber tube into the sterile slant medium at 28-32°C for 20-24 hours;

[0061] (2) Shake flask strains: insert the cultured slant strains into sterile shake flask seed medium at 28-32°C for 16-20 hours;

[0062] (3) Primary seed culture: Put the cultured shake flask seed liquid into the sterile primary seed medium, under the condition of 1:0.5 ventilation, stirring at 240rpm, and cultivate at 28-32°C for 12-16 hours;

[0063] (4) Secondary seed cultivation: Put the cultivated primary seed liquid into the sterile secondary seed medium, under the ventilation condition of 1:0.5, the stirring speed is 220rpm, and cultivate at 28-32℃ for 2-6 hours;

[0064] (5) Inoculate the cultured secondary seeds into the sterilized fermentation medium, under the ventilation condition of 1:0.3, the stirring speed is 140rpm, adjust the pH value to 7.0-7.2 with liquid ammonia during the fermentation...

Embodiment 2

[0091] A method for increasing the yield of L-glutamine, the method comprising the steps of:

[0092] (1) Preparation of fermentation broth:

[0093] Brevibacterium flavum MQG121 strain is used as the fermentation strain;

[0094] Slant culture: put the bacteria in the amp tube into the slant medium and cultivate at 28-32°C for 20-24 hours;

[0095] Shake flask strains: insert the cultured slant strains into the shake flask seed medium for 16-20 hours at 28-32°C;

[0096] First-level seed culture: put the cultured shake flask seed solution into the sterilized first-level seed medium and cultivate it for 12-16 hours at 28-32°C;

[0097] Secondary seed culture: put the cultivated primary seed solution into the sterilized secondary seed medium and cultivate it for 2-6 hours at 28-32°C; inoculate the cultivated secondary seed into the sterilized fermentation medium, Passing sterile air, adjusting the pH value to 6.2-6.8, and culturing for 40-48 hours to obtain a fermentation brot...

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Abstract

The invention belongs to the technical field of extraction and purification of amino acid derivative fermentation liquid, and in particular relates to a method for increasing the yield of L-glutamine. The method comprises the following steps: preparation of fermented liquid: slant culture, shake flask strain, primary seed cultivation, secondary seed cultivation, continuous fermentation; extraction and refining of fermented product: pretreatment of fermented liquid, secondary crystallization, decolorization and decolorization Miscellaneous treatment, membrane concentration and separation, drying, and packaging to obtain finished products. The invention has the beneficial effects of significantly reducing the content of bacteria, organic matter and inorganic impurities in the glutamine subsequent extraction and refining liquid, increasing the yield of glutamine; improving product quality, reducing production costs, and greatly reducing the labor burden of workers. Labor intensity; greatly reducing the discharge of water, acid, alkali, activated carbon and other solid and liquid waste, reducing environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of extraction and purification of amino acid derivative fermentation liquid, and in particular relates to a method for increasing the yield of L-glutamine. Background technique [0002] Glutamine (Glutamin), also known as glutamic acid, is the most abundant non-essential amino acid in the human body, and it is the only amino acid that can directly pass through the cerebrovascular barrier (BBB). In the human body, it is stored in skeletal muscle or blood. When injured or ill, glutamine may need to be obtained in sufficient quantities by consuming Gln-containing foods. Organisms catalyze the reaction of glutamic acid and ammonium salts to produce glutamine through glutamine synthetase. [0003] L-Glutamine, this product is used as nutritional supplement and flavoring supplement in food processing. And L-Glutamine is an important nutritional supplement for bodybuilders and bodybuilders. It (hereinafter refe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/14C07C237/06C07C231/24C12R1/13
Inventor 高树营王威李令娣
Owner SHANDONG MINQIANG BIOTECH
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