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A kind of extraction method of tylosin

A technology of tylosin and extraction method, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve problems such as inability to effectively remove impurities, and achieve high product titer, fast reaction speed and quality. stable effect

Active Publication Date: 2016-05-11
APELOA PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the shortcomings and deficiencies in the above-mentioned prior art, the inventor aims to provide a method for extracting tylosin, which can solve the problem that the usual stripping process cannot effectively remove impurities while ensuring high-quality and stable product potency. problem, on the one hand to solve the stripping solution with Ca(OH) 2 Neutralize a series of problems that arise

Method used

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  • A kind of extraction method of tylosin
  • A kind of extraction method of tylosin
  • A kind of extraction method of tylosin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for extracting tylosin, comprising the steps of:

[0038] Extraction: 3.0L of tylosin fermentation filtrate, the potency is 9534u / ml, and the content of component A is 68.4%. Add 0.75 L of butyl acetate each time, adjust the pH value to 10.0 with 10% NaOH, and extract twice at an extraction temperature of 30-35° C., stirring, and an extraction time of 30-60 minutes. After the extraction, the remaining aqueous phase was 3.05L with a titer of 527u / ml. The two extracts were combined to obtain 1.42 L of extract with a titer of 18436u / ml and an extraction yield of 91.5%.

[0039] Secondary stripping: take 1.4L of the extract, and primary stripping: add 48ml of tartaric acid with a concentration of 2% to adjust the pH value of the water phase to 5.5, and separate after standing for 1 hour. The lower aqueous phase is the primary stripping solution; The upper butyl acetate phase was subjected to secondary stripping: 86 ml of tartaric acid with a concentration of 1% w...

Embodiment 2

[0043] A method for extracting tylosin, comprising the steps of:

[0044] Extraction: 3.0L of tylosin fermentation filtrate, the potency is 10487u / ml, and the content of component A is 56.2%. Add 0.75 L of butyl acetate each time, adjust the pH value to 9.0 with 10% NaOH, and extract twice at an extraction temperature of 30-35° C., stirring, and an extraction time of 30-60 minutes. After the extraction, the remaining aqueous phase was 3.05L with a titer of 436u / ml. The two extracts were combined to obtain 1.41 L of extract with a titer of 20684u / ml and an extraction yield of 92.7%.

[0045] Secondary stripping: take 1.4L of the extract, and primary stripping: add 46ml of tartaric acid with a concentration of 2% to adjust the pH value of the water phase to 6.0, and separate the layers after standing for 1 hour. The lower aqueous phase is the primary stripping solution, which is treated with alkali crystallization; the upper layer of butyl acetate phase is subjected to seconda...

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Abstract

The invention belongs to the field of antibiotic drugs, and particularly relates to a tylosin extraction method, which mainly comprises: adopting a two-stage back-extraction manner to transfer tylosin to a water phase from an organic phase in stages, adding an alkali to the first-stage back-extraction solution to precipitate, separating the mother liquor, dissolving with the second-stage back-extraction solution, and carrying out neutralization, decoloration and drying through an ion exchange resin to obtain the product. According to the present invention, the high-quality and stable product potency is ensured, the problem that impurities can not be effectively removed with the usual back-extraction process is solved, and a series of problems appearing during the back-extraction solution neutralization process adopting Ca(OH)2 are solved.

Description

technical field [0001] The invention belongs to the field of antibiotic drugs, and in particular relates to a method for extracting tylosin. Background technique [0002] Tylosin (Tylosin) is a macrolide animal-specific antibiotic secreted by Streptomyces freundii, also known as Tylosin or Tylosin. The product is white to light yellow powder, easily soluble in methanol, soluble in ethanol, acetone, chloroform, slightly soluble in water, almost insoluble in hexane. Its salts are easily soluble in water, and the aqueous solution can be stored for 3 months at 25°C and pH 5.5 to 7.5 without reducing its effect. [0003] As a therapeutic drug, Tylosin is widely used in the prevention and treatment of mycoplasma disease, bacterial disease, spirochete disease and parasitic disease in livestock and poultry. It is the first choice drug for the treatment of mycoplasma disease in livestock and poultry. As a feed additive, it is widely used in the feed of chickens, pigs, cattle and ot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/08C07H1/08
Inventor 周晋武吕文进许永兴胡露华金康康
Owner APELOA PHARM CO LTD