Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nitrogen-hybridized mesoporous carbon-based biosensor, preparation method and application

A nitrogen-hybridized mesoporous carbon and biosensor technology, applied in the field of biosensors, can solve the problems of high price of gold electrodes, and achieve the effects of low detection cost, strong biological adaptability, and improved transmission capability.

Inactive Publication Date: 2016-07-27
HUNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, gold electrodes are expensive, and screen-printed electrodes are disposable, and the total cost for high-volume assays can be significant

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nitrogen-hybridized mesoporous carbon-based biosensor, preparation method and application
  • Nitrogen-hybridized mesoporous carbon-based biosensor, preparation method and application
  • Nitrogen-hybridized mesoporous carbon-based biosensor, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fabrication of biosensors based on nitrogen-hybridized mesoporous carbon

[0034]The nitrogen-hybridized mesoporous carbon-based biosensor of the present invention is a three-electrode electrochemical sensor, including a working electrode, a reference electrode and a counter electrode, the reference electrode is a saturated calomel electrode, and the counter electrode is a platinum electrode , the working electrode includes a glassy carbon electrode, and the detection end surface of the glassy carbon electrode is sequentially connected with L-cysteine, nitrogen hybridized mesoporous carbon, gold nanoparticle film and thiol-modified capture probe, the The gold nanoparticle film is also connected with mercaptoethanol that seals the remaining binding sites of the gold nanoparticle film.

[0035] The preparation method of described working electrode is,

[0036] 1. Surface treatment of the glassy carbon electrode: Polish the surface of the glassy carbon electrode, then rin...

Embodiment 2

[0046] Fabrication of biosensors based on nitrogen-hybridized mesoporous carbon

[0047] The nitrogen-hybridized mesoporous carbon-based biosensor of the present invention is a three-electrode electrochemical sensor, including a working electrode, a reference electrode and a counter electrode, the reference electrode is a saturated calomel electrode, and the counter electrode is a platinum electrode , the working electrode includes a glassy carbon electrode, and the detection end surface of the glassy carbon electrode is sequentially connected with L-cysteine, nitrogen hybridized mesoporous carbon, gold nanoparticle film and thiol-modified capture probe, the Also attached to the gold nanoparticle film is mercaptoethanol that blocks the remaining binding sites of the gold nanoparticle film.

[0048] The preparation method of the working electrode is the same as in Example 1, the difference is that in the second step, the concentration of the L-cysteine ​​solution is 1.0×10 -2 ...

Embodiment 3

[0053] The method for measuring the specific coding gene fragment of manganese peroxidase by the biosensor based on aza-hybridized mesoporous carbon, first drops samples containing signal probes and different concentrations of target chains on a nitrogen-hybridized mesoporous carbon-based biosensor. Base pairing is performed on the working electrode surface of the biosensor, and the reaction is not less than 60 minutes at 37°C; then the phosphate buffer solution (pH 7.38) was dropped on the surface of the working electrode of the nitrogen-hybridized mesoporous carbon-based biosensor, and the reaction was not less than 30 minutes at 37°C; finally, hydroquinone and hydrogen peroxide were used as substrates, and the three-electrode system was connected measured in an electrolytic cell. Utilize the measured current change value and the linear regression equation to calculate the content of the specific coding gene fragment of manganese peroxidase; the linear regression equation is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biosensor based on nitrogen-hybridized mesoporous carbon as well as a preparation method and application of the biosensor. The biosensor comprises a working electrode, wherein the working electrode comprises a glassy carbon electrode; the surface of the detecting end of the glassy carbon electrode is sequentially connected with an L-cysteine, nitrogen-hybridized mesoporous carbon, a gold nanoparticle film and a sulfydryl-modified capture probe; the gold nanoparticle film is also connected with mercaptoethannol. The invention also provides an application of the biosensor. The application comprises the following steps: dropwise adding an object chain contained liquid to be detected and a signal probe contained liquid on the surface of the working electrode; dropwise adding a nanogold cluster labeled horse radish peroxidase-streptavidin contained buffer solution; and measuring in an electrolytic tank with hydroquinone and hydrogen peroxide as substrates, wherein the electrolytic tank is connected with a three-electrode system. The biosensor is high in sensitivity, rapid in response, high in detecting precision, relatively strong in anti-interference, simple and convenient in application operation, high in efficiency and low in detecting cost.

Description

technical field [0001] The invention relates to a biosensor, in particular to a nitrogen-hybridized mesoporous carbon-based biosensor, a preparation method and an application thereof. Background technique [0002] In terrestrial ecosystems, lignin is an amorphous, aromatic polymer that widely exists in plants and contains oxyphenylpropanol or its derivatives in its molecular structure. Very common and accounting for 25% of woody plants, it is the second most abundant organic matter in the world after cellulose. The natural degradation rate of lignin is very slow, and it is usually disposed of by stacking or incineration, which not only produces such as CO x , methane and other harmful gases, while causing waste of resources, so biodegradation of lignin is more beneficial to environmental resources. White-rot fungi have the ability to biodegrade lignin. White-rot fungi can secrete some oxidative extracellular enzymes such as laccase, manganese peroxidase, and LiP to degrad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
Inventor 周耀渝汤琳曾光明陈俊蔡叶杨贵德王佳佳邓垚成方艳黎思思
Owner HUNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com