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Diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and preparation method thereof

A bovine serum albumin and nanoparticle technology, which is applied in the field of diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and its preparation, can solve the problem of inability to effectively and clearly track and observe drug treatment effects, inability to organically combine cancer diagnosis and treatment, and drug leakage. and other problems, to achieve the effects of flexible and easy-to-control regulation, improved diagnostic accuracy, and good degradability

Inactive Publication Date: 2014-04-30
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a kind of integrated bovine serum albumin anti-cancer nanoparticle and its preparation method based on the diagnosis and treatment based on pH response in order to overcome the defect existing in the above-mentioned prior art. When used as a drug carrier, there are problems such as low drug loading rate, drug leakage, inability to organically combine cancer diagnosis and treatment, and inability to effectively and clearly track and observe drug treatment effects, etc., and can prolong the half-life of drugs in the body, improve drug distribution in the body, and reduce Cleared by kidney or liver, protecting drug from degradation

Method used

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  • Diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and preparation method thereof
  • Diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and preparation method thereof
  • Diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and preparation method thereof

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Effect test

Embodiment 1

[0037] During the preparation process, add 15ml of secondary aqueous solution made of 750mg, 150mg, 75mg, and 15mg of bovine serum albumin to four 100ml beakers, and then add 0.2mmol of gadolinium chloride solution respectively, and put it on a magnetic stirrer at a uniform speed. Stir for 3 minutes, and the reaction temperature is room temperature 25°C. Then add 50ul, 1molL respectively -1 Sodium hydroxide aqueous solution, continue to stir for 2 hours, the solution forms a stable system, after 12 hours of dialysis and high-speed centrifugation, you can get nanoparticles with uniform particle size, such as figure 2 As shown, the particle size is around 10nm. Then freeze-dry and put 10 mg into a centrifuge tube to form an aqueous solution, add 2 mg of anticancer drug, and dialyze for 6 hours to remove the unloaded drug to obtain the drug-loaded nanoparticles, such as figure 1 , image 3 As shown, the particle size is relatively uniform. Take 5mg nanoparticles each to stud...

Embodiment 2

[0039] In the process of preparation, add 15ml of secondary aqueous solution made from 150mg of bovine serum albumin into four 100ml beakers, then add 0.2mmol, 0.15mmol, 0.1mmol, 0.05mmol of gadolinium chloride solution respectively, and place in a magnetic stirrer Stir at a constant speed for 3 minutes, and the reaction temperature is 25°C at room temperature. Then add 50ul, 1molL respectively -1 Sodium hydroxide aqueous solution, continue to stir for 2 hours, the solution forms a stable system, after 12 hours of dialysis and high-speed centrifugation, nanoparticles with uniform particle size can be obtained. Then 10 mg of each was lyophilized and placed in a centrifuge tube to form an aqueous solution, 2 mg of an anticancer drug was added, and the unloaded drug was removed by dialysis for 6 hours to obtain drug-loaded nanoparticles.

Embodiment 3

[0041]In the process of preparation, respectively add 15ml secondary aqueous solution that 150mg bovine serum albumin is made into in 3 100ml beakers, then add the gadolinium chloride solution of 0.15mmol respectively, stir 3min at a constant speed on the magnetic stirrer, the reaction temperature is Normal temperature 25°C. Then add 50ul, 40ul, 30ul 1mol L respectively -1 Sodium hydroxide aqueous solution, continue to stir for 2 hours, the solution forms a stable system, after 12 hours of dialysis and high-speed centrifugation, you can get nanoparticles of gadolinium trioxide with uniform particle size. Then 10 mg of each was lyophilized and placed in a centrifuge tube to form an aqueous solution, 2 mg of anticancer drug was added, and the unloaded drug was removed by dialysis for 6 hours to obtain nanoparticles loaded with anticancer drug.

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Abstract

The invention relates to a diagnosis and treatment integrated bovine serum albumin anticancer nanoparticle and a preparation method thereof. The particle comprises bovine serum albumin, rare earth metal ion and anticancer drug, wherein the rare earth metal ion and bovine serum albumin form nanoparticle with imaging function through coordination, the anticancer drug is immobilized on the nanoparticle and is bound to the rare earth metal ion coordination site that interacts with bovine serum albumin. Compared with the prior art, according to the invention, bovine serum albumin solution with certain concentration and rare earth metal ion solution form a compound particle through coordination. The structure and morphology of the compound particle can be regulated by changing the concentration of the two solutions to improve the particle performance. In addition, an element (rare earth metal ion) with MRI effect is introduced at the coordination site of the system. Compared with the traditional nanoparticle, the preparation method has the advantages of flexible and easily controllable regulation, and good repetitiveness, and the obtained nanoparticle has excellent properties.

Description

technical field [0001] The invention belongs to the field of macromolecule nano biomedical materials, and in particular relates to a bovine serum albumin anti-cancer nanoparticle integrated with diagnosis and treatment and a preparation method thereof. Background technique [0002] Cancer is a disease caused by the abnormal mechanism of cell growth and proliferation, which seriously endangers human health. In addition to growing out of control, cancer cells can also locally invade surrounding normal tissues, and even transfer to other parts of the body through the circulatory system or lymphatic system. According to statistics, 12 million people are diagnosed with cancer and 7.6 million people die from cancer every year, and the incidence of cancer is still increasing. It is estimated that there will be 26 million new cases and 17 million deaths in the world by 2030. people, most of which will occur in low- and middle-income developing countries. In some western countries,...

Claims

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Application Information

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IPC IPC(8): A61K49/14A61K47/48A61K9/19A61K45/00A61P35/00
Inventor 李永勇郑丽董海青朱海燕
Owner TONGJI UNIV
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