A high-efficiency microbial fertilizer
A high-efficiency microorganism and seed technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of fermentation metabolites, lack of investment in research, single microbial strains, etc., to improve soil aggregate structure, Improve soil fertility and improve soil physical properties
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Embodiment 1
[0026] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved on July 15, 2013 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: 1 Beichen West Road, Chaoyang District, Beijing, China) No. 3 Courtyard), the preservation number is CGMCCNo.7926.
[0027] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.
[0028] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.
[0029] Described bacterial strain characteristic is as follo...
Embodiment 2
[0090] A high-efficiency microbial fertilizer, the number of parts by weight is: 3 parts of Bacillus colloidus, 2 parts of phosphate solubilizing bacteria, 30 parts of Bacillus subtilis culture, 6 parts of Aspergillus awamori culture, and 12 parts of Lactobacillus plantarum.
[0091] Preparation method of Bacillus subtilis culture:
[0092] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liquid by step-by-step expansion culture of slanted strains;
[0093] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;
[0094] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;
[0095] (3) Tertiary seed cultivation: Inoculat...
Embodiment 3
[0107] A high-efficiency microbial fertilizer, the number of parts by weight is: 4 parts of Bacillus colloidus, 3 parts of phosphate solubilizing bacteria, 29 parts of Bacillus subtilis culture, 7 parts of Aspergillus awamori culture, and 13 parts of Lactobacillus plantarum.
[0108] The preparation of the Bacillus subtilis culture: transfer and cultivate the Bacillus subtilis from the inclined plane, transfer the seed liquid after step-by-step expansion into the fermentation tank, and after the fermentation is completed, the fermentation liquid is filtered and dried to obtain the Bacillus subtilis culture .
[0109] The preparation method of Lactobacillus plantarum agent: transfer and cultivate Lactobacillus plantarum from an inclined plane, transfer the seed liquid after step-by-step expansion into a fermenter, and after the fermentation is completed, the fermented liquid is concentrated to 45% of the original volume by low-temperature negative pressure vacuum to obtain Bact...
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