A high-efficiency microbial fertilizer

A high-efficiency microorganism and seed technology, applied in the direction of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of lack of fermentation metabolites, lack of investment in research, single microbial strains, etc., to improve soil aggregate structure, Improve soil fertility and improve soil physical properties

Active Publication Date: 2016-04-20
拜肯生物科技(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For many years, most of the processing methods of microbial fertilizers only use finished bacterial agents mixed with fillers, lacking fermentation metabolites, which seriously affect the fertilizer efficiency of microbial fertilizer products. For example, the patent application with publication number CN103396252A discloses a compound microorganism containing amino acids Fertilizer, in which the finished microbial agent is composed of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium mixed in a certain proportion
[0005] Moreover, the agricultural microbial agents currently on the market are mainly single microbial strains, which are difficult to restore the micro-ecological structure. At the same time, the synergy between common strains and crops is weak, which is unfavorable such as improving plant stress resistance and root nutrient absorption. For example, the publication number is The patent application of CN102888356A discloses a method and application of using Bacillus subtilis to prepare microbial fertilizer
Although some compound microbial agents have appeared on the market, their functions are single, or their use methods are limited, and the effect of increasing crop production after use is still unsatisfactory. Composite microbial agent or fertilizer with comprehensive functions and good yield-increasing effect
[0006] Due to the long-term lack of investment in microbial fertilizer research in China, my country's microbial fertilizer industry still has problems such as low overall level, insufficient technological innovation, and unstable product quality and application effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Bacillus subtilis (Bacillus subtilis) Li-2013-02, the strain has been preserved on July 15, 2013 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: 1 Beichen West Road, Chaoyang District, Beijing, China) No. 3 Courtyard), the preservation number is CGMCCNo.7926.

[0027] The characteristic of the strain is that it has high enzyme activity for producing high temperature-resistant α-amylase, and strong heat resistance and acid resistance.

[0028] The high temperature-resistant α-amylase enzyme activity prepared by the strain is 30000-35000u / ml; the applicable temperature range is 105-115°C, the optimum reaction temperature is 110°C, and the enzyme activity is completely stable at 110°C; the applicable reaction pH range is 3.0-7.0, when the pH value is 3.0, the enzyme activity is completely stable, and the optimum reaction pH value is 4.2.

[0029] Described bacterial strain characteristic is as follo...

Embodiment 2

[0090] A high-efficiency microbial fertilizer, the number of parts by weight is: 3 parts of Bacillus colloidus, 2 parts of phosphate solubilizing bacteria, 30 parts of Bacillus subtilis culture, 6 parts of Aspergillus awamori culture, and 12 parts of Lactobacillus plantarum.

[0091] Preparation method of Bacillus subtilis culture:

[0092] Obtaining of fermented liquid: Obtaining Bacillus subtilis fermented liquid by step-by-step expansion culture of slanted strains;

[0093] (1) Primary seed cultivation: Inoculate the slant strain of Bacillus subtilis into a 500-mL shaker flask with a medium capacity of 100 mL, a rotary shaker at 180 rpm, a cultivation temperature of 30°C, and a cultivation time of 24 hours;

[0094] (2) Secondary seed cultivation: Put the first-grade seeds into 500 ml second-grade seed shake flasks according to the inoculation amount of 10%, and the cultivation conditions are the same as the first-grade seeds;

[0095] (3) Tertiary seed cultivation: Inoculat...

Embodiment 3

[0107] A high-efficiency microbial fertilizer, the number of parts by weight is: 4 parts of Bacillus colloidus, 3 parts of phosphate solubilizing bacteria, 29 parts of Bacillus subtilis culture, 7 parts of Aspergillus awamori culture, and 13 parts of Lactobacillus plantarum.

[0108] The preparation of the Bacillus subtilis culture: transfer and cultivate the Bacillus subtilis from the inclined plane, transfer the seed liquid after step-by-step expansion into the fermentation tank, and after the fermentation is completed, the fermentation liquid is filtered and dried to obtain the Bacillus subtilis culture .

[0109] The preparation method of Lactobacillus plantarum agent: transfer and cultivate Lactobacillus plantarum from an inclined plane, transfer the seed liquid after step-by-step expansion into a fermenter, and after the fermentation is completed, the fermented liquid is concentrated to 45% of the original volume by low-temperature negative pressure vacuum to obtain Bact...

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Abstract

The invention discloses a high-efficiency microbial fertilizer, belonging to the technical field of agricultural fertilizers. The high-efficiency microbial fertilizer is composed of 2-5 parts of Bacillus mucilaginosus, 1-3 parts of phosphate solubilizing bacteria, 20-35 parts of Bacillus subtilis culture, 6-8 parts of Aspergillus awamori culture and 10-15 parts of Lactobacillus plantarum inoculum. The fertilizer can improve the soil; the beneficial microbes in the fertilizer can generate saccharides and plant mucilage; the mineral embryo can be combined with the organic colloids to improve the crumb structure of the soil, enhance the physical properties of the soil and reduce the loss of the soil particles; and the fertilizer can participate in humification under certain conditions, thereby being beneficial to increasing the soil fertility. The product is simple in application method, the consumption is 0.3-1kg per mou, the cost is only 70-100 Yuan / mou, and the product is sprayed on the field surface before irrigation in the seed treatment or growth period.

Description

technical field [0001] The invention relates to a biological fertilizer processing method, which belongs to the technical field of agricultural fertilizers, in particular to a high-efficiency microbial fertilizer and its preparation. Background technique [0002] Microbial fertilizer is a microbial living product that uses microbial life activities and its products to cause crops to obtain specific fertilizer effects. It can improve soil fertility, improve fertilizer utilization, inhibit crops from absorbing harmful substances such as heavy metals and pesticides, and purify and restore soil. It plays an irreplaceable role in promoting the utilization of crop stalks and urban waste, and improving the quality of agricultural products. The efficacy of microbial fertilizers is mainly achieved through the synergistic effect on traditional chemical fertilizers and organic fertilizers, the improvement and activation of soil, and the physiological effects of microorganisms. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12R1/07C12R1/125C12R1/665C12R1/25
Inventor 郭建峰梁栋马忠平张艳玲
Owner 拜肯生物科技(上海)股份有限公司
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