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Method for preparing feed additive by using nisin extraction waste liquid

A nisin and feed additive technology, applied in animal feed, animal feed, application, etc., can solve the problems of difficulty in sewage treatment, waste of resources, etc., and achieve the effects of clean production, pollution avoidance, and increased production costs

Active Publication Date: 2014-05-21
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The supernatant solids contain more than 90% sodium chloride, a small amount of other inorganic salts, and low-molecular sugars. If they are directly discharged, it will bring difficulties to sewage treatment and cause waste of resources.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Bacteria: Lactococcus

[0018] Seed medium (g / L): 5 sucrose, 10 yeast powder, 5 sodium chloride, 2 potassium dihydrogen phosphate, 0.2 magnesium sulfate;

[0019] Fermentation medium (g / L): sucrose 70 (batch feeding), yeast powder 5, corn steep liquor 10, sodium chloride 5, potassium dihydrogen phosphate 2, magnesium sulfate 0.2, defoamer 0.2;

[0020] Culture conditions: 30°C, anaerobic culture;

[0021] After 24 hours of fermentation, the nisin titer reached 6800IU / mL, and the tank volume was 10T. The pH of the fermentation broth was adjusted to 3.0, and then heat-treated at 80°C for 20 minutes. Cool with cooling water, and start ceramic membrane filtration when the temperature drops to 50°C. Filtrate with 50000Da ceramic membrane, adjust the frequency of the main pump and the opening of the stop valve, control the membrane inlet pressure to 0.3MPa, and control the permeate flow rate to about 30L / min. After 5 hours of filtration, stop when the remaining volume is 5...

Embodiment 2

[0029] The seed and fermentation medium adopt the medium formulation of Example 1, and the culture conditions are as follows: during the fermentation process, the pH is controlled between 6.5 and 7.0, the sugar content is controlled below 25 g / L, and cultured with slight aeration. After 18 hours of fermentation, the potency reached 7600IU / mL, and the volume in the tank was 8T. The fermentation broth was adjusted to pH 2.5, treated at 90°C for 5 minutes, then cooled to 60°C and filtered through a 50,000Da ceramic membrane, and stopped when the final remaining volume was 500L. Then wash with pH 2.6 water three times, adding 500L each time. Add 40L of natamycin extraction waste liquid to the washed bacteria suspension, and spray dry to obtain 40kg of bacteria powder with a protein content of up to 89%.

[0030] The ceramic membrane permeate is concentrated 10 times by the ultrafiltration membrane, 25% sodium chloride is added for salting out for 2 hours, 100L natamycin waste liq...

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Abstract

The invention provides a method for preparing a feed additive by using a nisin extraction waste liquid, belonging to the technical field of the fermentation industry. The method comprises a first step of using a ceramic membrane for circulating filtration so as to intercept thalli and a second step of drying a material liquid containing high-concentration thalli so as to prepare mycoprotein powder and is mainly used in the field of feeds or agricultural fertilizers. With the method, the recovery rate of intercepted protein is high, macromolecular protein in a medium can be intercepted except mycoprotein, and abundant nutrients are obtained. A liquid permeated from the ceramic membrane is subjected to ultrafiltration and concentration and then to salting-out so as to prepare high-purity nisin. A supernatant obtained after salting-out contains considerable sodium chloride and is subjected to spray drying, and an obtained substance is used as a feed additive in the feed industry. Thus, the nisin extraction waste liquid is turned from the waste into the valuable, pollution to the environment caused by discharging of the waste liquid is prevented, sustainable development of the fermentation industry is realized, and a feasible approach is provided for clean production.

Description

technical field [0001] The invention relates to a method for preparing a feed additive by using nisin to extract waste liquid, and belongs to the technical field of fermentation industry. Background technique [0002] The development of non-grain feed sources has always been the research direction of the feed industry, which can solve the contradiction between humans and animals earning food. The use of waste liquid extracted from fermentation plants to prepare feed additives can reduce the application of grain in the feed industry, achieve the effect of rational use of resources and turning waste into treasure, and open up a clean production road for the sustainable development of the fermentation industry. [0003] The production of nisin is closely related to the growth of the strain, that is, the yield of nisin depends on the growth rate of the strain. After the fermentation, the amount of bacteria is relatively large, and the first step of downstream extraction is to re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C05F11/08
Inventor 康传利苏移山朱希强袁艳华张晓元凌沛学
Owner SHANDONG FREDA BIOTECH
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