Selenium-enriched yeast and preparation method thereof

A selenium-enriched yeast and selenium-enriched technology, applied in the direction of fungi, etc., can solve the problems that are not conducive to the production of fermented food, unavoidable residues, human injury, etc., and achieve the effects of full utilization, high conversion rate, and improved safety

Active Publication Date: 2014-05-28
牡丹江灵泰药业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example: Chinese patent application No. 01100600.5 "Selenium-rich yeast with high biomass and its production method", Chinese patent application No. 94105548.5 "Selenium-rich yeast and its production process", Chinese patent application No. 200610066369.8 "Selenium-rich Saccharomyces cerevisiae, Selenium-enriched yeast products and their preparation methods” and so on, all aim to achieve the purpose of selenium-enriched yeast by improving the carbon source, nitrogen source and other nutrients of the medium and the selenium-tolerant acclimation process, and do not involve the source of selenium.
[0004] In addition, selenate and selenite are highly toxic inorganic salts, which will definitely cause harm to the human body to a certain extent in the process of large-scale production.
At the same time, selenate and selenite that have not been completely converted into organic selenium are still difficult to avoid remaining after treatment, which is not conducive to the production of fermented food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The preparation of embodiment 1 selenium-enriched yeast

[0023] (1) Select 100g of carbonaceous shale selenium ore with a uniform particle size and a diameter between 40-100 mesh, soak it in 300mL sulfurous acid solution (concentration: 15%, v / v) for 24 hours, and use ultrasonic vibration at the same time, Accelerate the dissolution of selenium ore;

[0024] (2) Take 150mL sodium phosphate solution (10% concentration, w / v) to precipitate heavy metals in selenium ore, and filter;

[0025] (3) Adjust the pH of the filtrate to 6.0 with 0.1mol / L NaOH solution;

[0026] (4) Add 10g of yeast powder, 20g of peptone, and 20g of glucose to prepare a liquid medium, which is divided into Erlenmeyer flasks, with a volume of 50mL / 250mL;

[0027] (5) Sterilize the prepared medium at 121°C for 20 minutes;

[0028] (6) Take out the sterilized culture solution, and the selenium concentration in the culture solution is 20 μg / ml. Inoculate yeasts from three sources prepared in advanc...

Embodiment 2

[0031] Example 2 Preparation of selenium-enriched yeast

[0032] (1) Select 150g of carbonaceous shale selenium ore with uniform particle size and diameter between 40-100 mesh, soak in 350mL sulfurous acid solution (concentration: 20%, v / v) for 48h, and use ultrasonic vibration at the same time , to accelerate the dissolution of selenium ore;

[0033] (2) Take 200mL of sodium phosphate solution (concentration: 15%, w / v) to precipitate heavy metals in selenium ore, and filter;

[0034] (3) Dilute the filtrate with 0.1mol / L Na 2 CO 3 Adjust the pH of the solution to 6.0;

[0035] (4) Add 10g soybean flour, 20g peptone, and 20g sucrose to make a liquid medium, and divide it into Erlenmeyer flasks, with a volume of 50mL / 250mL;

[0036] (5) Sterilize the prepared medium at 121°C for 20 minutes;

[0037] (6) Take out the sterilized culture medium, the selenium concentration in the culture medium is 18 μg / ml, and the yeasts from the three sources prepared in advance (respectivel...

Embodiment 3

[0040] Example 3 Preparation of selenium-enriched yeast

[0041] (1) Select 200 g of carbonaceous shale selenium ore with a uniform particle size and a diameter between 40 and 100 mesh, and soak in 400 mL of sulfurous acid solution (20% concentration, v / v) for 48 hours. At the same time, ultrasonic vibration is used to accelerate the dissolution of selenium ore;

[0042] (2) Take 200mL of sodium phosphate solution (10% concentration, w / v) to precipitate heavy metals in selenium ore;

[0043] (3) Adjust the pH of the filtrate to 6.0 with 0.1mol / L KOH solution;

[0044] (4) Add 10g of wheat bran juice, 20g of peptone, and 20g of corn flour to make a liquid medium, and divide it into Erlenmeyer flasks with a volume of 50mL / 250mL. ;

[0045] (5) Sterilize the prepared medium at 121°C for 20 minutes;

[0046] (6) Take out the sterilized culture medium, the selenium concentration in the culture medium is 22 μg / ml, and three strains of yeast from three sources prepared in advance...

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PUM

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Abstract

The invention discloses a selenium-enriched yeast and a preparation method thereof. The selenium-enriched yeast is obtained by cultivating microzyme in a selenium-enriched culture medium. The preparation method comprises the steps as follows: selenium ore is immersed in an acid liquid to dissolve element selenium; heavy metal is subjected to sedimentation treatment via a phosphoric acid slat solution, and filtration is performed; the pH value of a filtrate is regulated to 5.5-6.5; a carbon source and a nitrogen source which are necessary for the growth of the microzyme are added to obtain the selenium-enriched culture medium; the obtained selenium-enriched culture medium is sterilized; the microzyme is inoculated and cultivated; microzyme bodies are collected, frozen and dried in a vacuum condition to obtain selenium-enriched yeast. According to selenium-enriched yeast and the preparation method thereof, the selenium ore is adopted as a source of the selenium element so as to broaden the selenium source material for the selenium-enriched yeast, and improve the safety of the product.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a food-grade selenium-enriched yeast and a preparation method thereof. Background technique [0002] Yeast, a microorganism with the largest direct consumption by humans. Yeast cells are rich in protein, fat, sugar and B vitamins, as well as enzymes, coenzymes, ribonucleic acid, sterols and some metabolic intermediates. Selenium is an essential trace element for the human body. It has very important physiological functions and is closely related to human health. Selenium can prevent and inhibit tumors, anti-aging, maintain the normal function of the cardiovascular system, and prevent the occurrence of arteriosclerosis and coronary heart disease. Selenium deficiency can lead to Keshan disease and osteoarthritis, and can also induce cataract, liver disease and pancreatic disease and so on. Selenium-enriched yeast can be used as raw materials for selenium-supplementing he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16
Inventor 李冬生汪超祁勇刚石勇王金华高冰
Owner 牡丹江灵泰药业股份有限公司
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