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Macroporous three-dimensional ordered orientation silk fibroin nanofiber scaffold and preparation method thereof

A nanofiber, silk fibroin technology, applied in the chemical post-processing of cellulose/protein rayon, single-component fibroin rayon, fiber processing, etc., can solve a lot of time, limited application, small size and other problems, to achieve the effect of good mechanical and processing performance, good biological activity, and low manufacturing cost

Active Publication Date: 2016-04-13
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the fiber material obtained by parallel electrodes has good fiber orientation, but its small size limits its application.
The size of the fiber material collected by the drum is large, but the fiber orientation is not good enough. To improve the orientation, the drawing force needs to be increased, and the fibers with poor mechanical properties are easily broken.
In addition, like traditional spinning, it can only be made into dense, thin fiber membranes
Chen et al. obtained a three-dimensional orderly scaffold in which cells grow into it by accumulating one layer of cells and one layer of scaffolding. However, this scaffold is complicated to manufacture and requires a lot of time to operate, and the operational time of cells is limited. Further improvement Thickness is more difficult
In addition, this kind of scaffold is the accumulation of very thin single-layer scaffolds, and the material has poor consistency and uniformity. It must borrow external force to maintain its shape, which limits its application in vivo (Chen, X., X.Fu, et al. "Regulation of the osteogenesis of pre-osteoblasts by spatial arrangement of electrospunnanofibers in two -and three-dimensional environments."Nanomedicine: Nanotechnology, Biology and Medicine)

Method used

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  • Macroporous three-dimensional ordered orientation silk fibroin nanofiber scaffold and preparation method thereof
  • Macroporous three-dimensional ordered orientation silk fibroin nanofiber scaffold and preparation method thereof
  • Macroporous three-dimensional ordered orientation silk fibroin nanofiber scaffold and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Cut silkworm cocoons into 1cm 2 The small pieces were boiled with 500mL of 0.5% sodium carbonate solution for 1.5 hours, cleaned with deionized water, boiled in 500mL of deionized water for 0.5h, washed with deionized water and dried. Add about 60mL of calcium chloride / water / ethanol three-phase solution (the molar ratio of the three substances is 1 / 8 / 2) to the treated silk, stir in a water bath at 70°C for 1 hour to completely dissolve the silk, and then add the obtained The solution was dialyzed for 48 hours. After the dialysis, the solution was centrifuged at 8000r / min for 30 minutes and then filtered. The filtered solution was frozen in liquid nitrogen and then dried for 48 hours to obtain regenerated silk fibroin.

[0040] 2. Dissolve regenerated silk fibroin in a mixed solution of dichloromethane and trifluoroacetic acid with a mass ratio of 3:7 to prepare a solution for electrospinning with a mass fraction of regenerated silk fibroin of 6%.

[0041] 3. Spinni...

Embodiment 2

[0047] 1. Cut silkworm cocoons into 1cm 2 The small pieces were boiled with 500mL of 0.5% sodium carbonate solution for 1.5 hours, cleaned with deionized water, boiled in 500mL of deionized water for 0.5h, washed with deionized water and dried. Add about 60mL of calcium chloride / water / ethanol three-phase solution (the molar ratio of the three substances is 1 / 8 / 2) to the treated silk, stir in a water bath at 70°C for 1 hour to completely dissolve the silk, and then add the obtained The solution was dialyzed for 48 hours. After the dialysis, the solution was centrifuged at 8000r / min for 30 minutes and then filtered. The filtered solution was frozen in liquid nitrogen and then dried for 48 hours to obtain regenerated silk fibroin.

[0048] 2. Dissolve regenerated silk fibroin in a mixed solution of dichloromethane and trifluoroacetic acid with a mass ratio of 3:7 to prepare a solution for electrospinning with a mass fraction of regenerated silk fibroin of 20%.

[0049] 3. Spinn...

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Abstract

The invention provides a macropore three-dimensional ordered-orientation silk fibroin nano-fiber support and a preparation method thereof. The macropore three-dimensional ordered-orientation silk fibroin nano-fiber support is realized through an ethyl alcohol bath roller collecting method by mainly applying an electrostatic spinning method. The ethyl alcohol bath roller collecting method is characterized in that a part of a conducting roller is immersed in ethyl alcohol, and the ethyl alcohol has low surface tension, so that the prepared silk fibroin nano-fiber can be soaked and dispersed in the ethyl alcohol, and gaps between filaments are enlarged, and meanwhile, the roller can make rotational motion, so that the collected filaments can be configured in sequence toward one direction. The silk fibroin nano-fiber support prepared through the preparation method provided by the invention is characterized in that the diameter of each fiber in the fiber support is 10nm-10[mu]m, the fibers are arranged in an ordered-orientation manner, the average angle between every fibers is 0-30 degrees, the fiber support has a three-dimensional structure and is 0.05-5mm in thickness, and the bore diameter between every two fibers is 0-30[mu]m.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffold materials, and more specifically relates to a macroporous three-dimensional ordered orientation silk fibroin nanofiber scaffold and a preparation method thereof. Background technique [0002] Tissues and organs are damaged to varying degrees due to disease trauma, and part of the tissue damage is difficult or even impossible to fully recover through the body's self-healing ability. Tissue engineering is to study how to promote damage repair and even achieve the purpose of tissue and organ regeneration. Among them, the scaffold as the carrier of cells, growth factors and drugs is one of the key factors in tissue engineering. The ideal scaffold material is one that mimics the extracellular matrix (ECM) composition and structure of tissue, thereby simulating the extracellular environment to promote cell growth. [0003] Natural extracellular matrix components are used by many people to ma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/4266D04H1/76D01D5/00D01F4/02D01F11/02A61L27/22A61L27/56
Inventor 王家伟丁慧芬
Owner WUHAN UNIV