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Trichoderma harzianum and applications thereof in preventing and controlling turfgrass brown patch disease

A brown spot and lawn technology, applied to a strain of Trichoderma harzianum and its application in the prevention and control of lawn brown spot, can solve problems such as high cost, easy pollution of the environment, chemical drug resistance and even drug resistance , to achieve the effect of strong survivability, wide adaptability and great application value

Inactive Publication Date: 2014-07-30
TANGSHAN JINTU MICROBIAL ORGANIC FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control is not only costly, but also easy to pollute the environment. While preventing and controlling pathogenic bacteria, pathogenic bacteria are also prone to develop drug resistance or even drug resistance to chemical agents.

Method used

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  • Trichoderma harzianum and applications thereof in preventing and controlling turfgrass brown patch disease
  • Trichoderma harzianum and applications thereof in preventing and controlling turfgrass brown patch disease
  • Trichoderma harzianum and applications thereof in preventing and controlling turfgrass brown patch disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, acquisition, identification and preservation of bacterial strains

[0030] PDA medium: 200g potato, 15g glucose, 20g agar, 1000ml distilled water, sterilized at 121°C for 20min.

[0031] TSM medium: MgSO 4 ·7H 2 00.2g, K 2 HPO 4 0.9g, KCl0.15g, NH 4 NO 3 1.0g, glucose 3.0g, rose bengal 0.15g, 60% dexon wettable powder 0.3g, PCNB 0.2g, agar 20g, distilled water 950ml, sterilized at 121°C for 20min.

[0032] 1. Obtaining JTM53 strain

[0033] Sampling time: February 2008.

[0034] Sampling location: Beijing golf course.

[0035] Remove 3-5cm of topsoil, then dig out the root system of the plant, put it together with the soil around the root into a polyethylene plastic bag, and bring it back to the laboratory. Air-dry the root system attached to the soil slightly, pat the root system lightly to make the soil attached to it fall off, dilute it with sterile water gradient, absorb 0.1mL of the diluted solution and drop it on the TSM medium plate, and us...

Embodiment 2

[0044] Embodiment 2, the inhibitory action of JTM53 bacterial strain to pathogenic bacteria

[0045] 1. Inoculate a single colony of JTM53 strain on a PDA medium plate and culture at 20°C for 5 days.

[0046] 2. Take the PDA medium plate that has completed step 1, and use a puncher to get a bacterium cake (biocontrol bacterium cake) with a diameter of 5 mm at the front of the colony.

[0047] 3. Inoculate a single colony of Rhizoctonia solani on a PDA medium plate and culture at 20°C for 5 days.

[0048] 4. Get the PDA culture medium plate that has completed step 3, and use a puncher to get a bacterium cake (pathogenic bacterium cake) with a diameter of 5 mm at the front of the colony.

[0049] 5. Take a new PDA medium plate, and use a puncher to punch out a plate with a diameter of 5 mm, as a control.

[0050] 6. Group processing

[0051] Test group: Take a new PDA medium plate, place 1 biocontrol cake and 1 pathogen cake on it, the linear distance between the centers of the...

Embodiment 3

[0055] Embodiment 3, JTM53 bacterial strain is to the reparasitic ability of pathogenic bacteria

[0056] 1. Inoculate a single colony of JTM53 strain on a PDA medium plate and culture at 20°C for 5 days.

[0057] 2. Take the PDA medium plate that has completed step 1, and use a puncher to get a bacterium cake (biocontrol bacterium cake) with a diameter of 5 mm at the front of the colony.

[0058] 3. Inoculate a single colony of Rhizoctonia solani on a PDA medium plate and culture at 20°C for 5 days.

[0059] 4. Get the PDA culture medium plate that has completed step 3, and use a puncher to get a bacterium cake (pathogenic bacterium cake) with a diameter of 5 mm at the front of the colony.

[0060] 5. Take a new PDA medium plate, place a 1cm×3cm cellophane strip on it, then place a biocontrol cake at one end of the length of the cellophane strip, and place a pathogenic bacteria cake at the other end, at 25°C Culture (12h light / 12h dark). After observing the mycelium contac...

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Abstract

The invention discloses trichoderma harzianum and applications thereof in preventing and controlling a turfgrass brown patch disease. The collection number of the trichoderma harzianum JTM53 is CGMCC (China General Microbiological Culture Collection Center) No.8914. The invention further protects a fermentation broth of a JTM53 strain. The JTM53 strain has the attributes of being strong in survivability, wide in adaptability, free from environmental pollution and the like, thus having wide application prospects as a biocontrol bacterium. The indoor co-antagonism experiments show that the JTM53 strain has an excellent antibacterial effect on rhizoctonia solani. Furthermore, the JTM53 strain is used for preparing a solid fermentation matter, and then the solid fermentation matter is further used for potted plant prevention and control experiments and land controlling experiments, and the prevention and control effects on rhizoctonia solani are excellent and stable. The trichoderma harzianum has important application values on the prevention and control of the turfgrass brown patch disease.

Description

technical field [0001] The invention relates to a strain of Trichoderma harzianum and its application in preventing and controlling lawn brown spot disease. Background technique [0002] Lawn brown spot is an important soil-borne fungal disease caused by Rhizoctonia solani and occurs on lawns. The disease commonly occurs on lawns of cool and warm seasons at home and abroad, and seriously harms the growth and development of lawns, and even causes devastating damage to lawns in some years. The disease can also cause disease of a variety of crops in different regions, causing a large amount of economic losses. [0003] Lawn grasses are mostly perennial, which provides suitable conditions for the accumulation of Rhizoctonia solani and the occurrence of lawn brown spot. The typical symptom of lawn brown spot disease is that turfgrass can cause root, stem and leaf tissue to rot or a large number of plants to die within a short period of time after being infected. So far, no effe...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 姚彦坡王禹王洪瑛朱少伦王学民
Owner TANGSHAN JINTU MICROBIAL ORGANIC FERTILIZER
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