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Method for preparing total saponins of radix cynanchi bungei

A technology of Baishouwu and total saponins, which is applied in the field of preparation of Baishouwu total saponins, can solve the problems that it is difficult to control the change of the solvent concentration of the extract, the change of the extraction temperature, and the long extraction time, etc., and shorten the processing time. The effects of time, accelerated dissolution, and convenient operation

Active Publication Date: 2014-08-06
江苏博益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For extracting the total saponins of Radix Polygoni Multiflori, it has its limitations, such as Soxhlet extraction, it is difficult to control the change of the solvent concentration of the extract; leakage extraction cannot control the change of the extraction temperature; and the heating reflux extraction method can control Extraction solvent concentration, but only after 2 or 3 extractions can the saponins be extracted more completely, requiring more extraction solvents, and the extraction time is longer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Dry and pulverize Baishouwu, pass through a 20-mesh sieve, take 1kg of the powder, and add it to 1kg of acetic acid / sodium acetate containing biological enzymes (cellulase and hemicellulase at a mass ratio of 2:1) with a pH of 4.0 Enzymolysis at 40°C for 2 hours in a buffer solution; put the material after enzymolysis in an ultrasonic device, add 3kg of ethanol solution with a mass fraction of 60%, and extract it under the action of ultrasonic waves with a frequency of 40KHz and a power of 250W. The extraction temperature is 70°C, the extraction time is 60min, and centrifuged to obtain the extract; the extract is ultrafiltered through an ultrafiltration membrane with a molecular cut-off value of 3000 until the volume of the concentrate is reduced to 1 / 3 of the original; the concentrate is passed through AB-8 macroporous adsorption resin column adsorption, first use 2 times the column volume of water to elute the impurities, and then use 3 times the column volume of 50% e...

Embodiment 2

[0018] Dry and crush Baishouwu, pass through a 20-mesh sieve, take 5kg of powder, and add it to 10kg of acetic acid / sodium acetate containing biological enzymes (cellulase and hemicellulase at a mass ratio of 2:1) with a pH of 4.5 Enzymolysis at 50°C for 3 hours in a buffer solution; put the material after enzymolysis in an ultrasonic device, add 25kg of ethanol solution with a mass fraction of 70%, and extract it under the action of ultrasonic waves with a frequency of 40KHz and a power of 250W. The extraction temperature is 75°C, the extraction time is 90min, and centrifuged to obtain the extract; the extract is ultrafiltered through an ultrafiltration membrane with a molecular cut-off value of 4000 until the volume of the concentrate is reduced to 1 / 5 of the original; the concentrate is passed through D-101 macroporous adsorption resin column adsorption, first use 3 times the column volume of water to elute the impurities, and then use 5 times the column volume of 60% ethano...

Embodiment 3

[0020] Dry and crush Baishouwu, pass through a 20-mesh sieve, take 5kg of powder, and add it to 5kg of acetic acid / sodium acetate containing biological enzymes (cellulase and hemicellulase at a mass ratio of 2:1) with a pH of 5.0 Enzymolysis at 45°C for 2.5 hours in a buffer solution; put the material after enzymolysis in an ultrasonic device, add 20kg of ethanol solution with a mass fraction of 80%, and extract under the action of ultrasonic waves with a frequency of 40KHz and a power of 250W , the extraction temperature is 80°C, the extraction time is 120min, and centrifuged to obtain the extract; the extract is ultrafiltered through an ultrafiltration membrane with a molecular cut-off value of 5000 until the volume of the concentrate is reduced to 1 / 3 of the original; the concentrate Adsorbed by DS-401 macroporous adsorption resin column, first elute impurities with 2 times column volume of water, then change to 6 times column volume of 70% ethanol, collect the eluate, and u...

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Abstract

The invention relates to a method for preparing total saponins of radix cynanchi bungei. The method comprises the following steps: (1) on the basis of using the radix cynanchi bungei as raw material, drying, crushing, sieving, and carrying out biological enzyme digestion; (2) carrying out ultrasonic extraction; (3) ultra-filtering and concentrating the extracting solution; (4) further purifying the concentrated solution by macroporous adsorption resin; concentrating and drying so as to obtain the total saponins of the radix cynanchi bungei. The total saponins of the radix cynanchi bungei prepared by the invention has the characteristics of high yield, simplified production process, short production cycle, high product purity, and the like, and is easy for realization of industrialization enlargement.

Description

technical field [0001] The invention relates to a preparation method of total saponins of Radix Polygoni Multiflori, belonging to the field of extraction and separation of effective components of Chinese herbal medicines. Background technique [0002] The main source of Baishouwu is the root tuber of Niupixiao, a plant of the genus Auricularia velvet, which is a traditional tonic in Chinese medicine. It has the effects of nourishing liver and blood, strengthening kidney and essence, strengthening muscles and bones, and The function of invigorating the spleen and replenishing qi is used to treat insomnia and forgetfulness, premature graying of the beard and hair, poor muscles and bones, chest tightness and heartache, and indigestion. Its main active ingredient, total saponins of Radix Polygoni Multiflori, has various pharmacological functions. In vitro experiments have proved that the extract of Baishouwu has the function of scavenging oxygen free radicals, and further exper...

Claims

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Application Information

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IPC IPC(8): A61K36/27A61K125/00
Inventor 范笑平陈洪生陈程程星源
Owner 江苏博益生物科技有限公司
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