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Polygala japonica polysaccharide extracted from polygala japonica and application of polygala japonica polysaccharide

A technology of melon seed gold polysaccharide and melon seed gold, applied in the field of medicine, to achieve the effect of enhancing the immune function of the body, huge economic and social benefits, and simple extraction process

Inactive Publication Date: 2014-08-27
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Melon seed gold contains saponins, organic acids, flavonoids, oligosaccharide esters and other components [2]. Modern research on melon seed gold mainly focuses on saponins [3-4]. The activity of enhancing immune function has not been reported

Method used

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  • Polygala japonica polysaccharide extracted from polygala japonica and application of polygala japonica polysaccharide
  • Polygala japonica polysaccharide extracted from polygala japonica and application of polygala japonica polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0009] Take melon seed gold and crush it through a No. 2 sieve, add water according to the solid-liquid weight ratio of 1:10, and stir evenly; extract twice in a water bath at 70°C, each time for 1 hour, filter, combine the filtrates, and concentrate to a solid-liquid volume ratio of 1:1 to obtain Polysaccharide concentrate: add ethanol with a mass concentration of more than 95% to the polysaccharide concentrate and stir well until the alcohol content is 80%, leave it at 4°C for 12 hours, centrifuge, and elute with absolute ethanol, acetone, and ether in sequence , to obtain crude polysaccharide; add water to the obtained crude polysaccharide to make the crude polysaccharide concentration 3mg / mL, put it on the AB-8 resin column, the column diameter height ratio is 1:4, the sample loading is 2BV, and the elution flow rate is 1ml / min, Elute with 8BV water, concentrate to a relative density of 15mg / mL, add ethanol with a mass concentration of 95% or more to an alcohol content of 8...

Embodiment 2

[0011] Take melon seed gold and crush it through a No. 2 sieve, add water according to the solid-liquid weight ratio of 1:20, and stir evenly; extract in a water bath at 85°C for 3 times, each time for 2 hours, filter, combine the filtrates, concentrate to a solid-liquid volume ratio of 1:2, and obtain Polysaccharide concentrate: add ethanol with a mass concentration of more than 95% to the polysaccharide concentrate and stir well until the alcohol content reaches 85%. After standing at 4°C for 18 hours, centrifuge and elute with absolute ethanol, acetone, and ether in sequence , to obtain crude polysaccharides; add water to the obtained crude polysaccharides to make the concentration of crude polysaccharides 8mg / mL, put on the AB-8 resin column, the column diameter to height ratio is 1:8, the sample loading is 3BV, and the elution flow rate is 1ml / min, Elute with 9BV water, concentrate to a relative density of 23mg / mL, add ethanol with a mass concentration of 95% or more to an...

Embodiment 3

[0013] Take melon seed gold and crush it through a No. 2 sieve, add water according to the solid-liquid weight ratio of 1:30, and stir evenly; extract twice in a water bath at 100°C, each time for 3 hours, filter, combine the filtrates, and concentrate to a solid-liquid volume ratio of 1:3 to obtain Polysaccharide concentrate: add ethanol with a mass concentration of more than 95% to the polysaccharide concentrate and stir well until the alcohol content is 90%, leave it at 4°C for 24 hours, centrifuge, and elute with absolute ethanol, acetone, and ether in sequence , to obtain crude polysaccharide; add water to the obtained crude polysaccharide to make the concentration of crude polysaccharide be 12mg / mL, put it on the AB-8 resin column, the column diameter height ratio is 1:12, the sample loading is 4BV, and the elution flow rate is 1ml / min, Elute with 10BV of water, concentrate to a relative density of 30 mg / mL, add ethanol with a mass concentration of 95% or more to an alcoh...

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Abstract

The invention relates to polygala japonica polysaccharide extracted from polygala japonica and the application of the polygala japonica polysaccharide. Extraction of the polygala japonica polysaccharide can be effectively achieved, and medicine and health care products which can enhance the human immunologic function can be effectively prepared. According to the technical scheme, polygala japonica medicine is taken and smashed, and water is added; water bathing extraction is carried out 2-3 times, filtering is carried out, filter liquor is merged, concentration is carried out, and polysaccharide concentrated liquor is obtained; ethyl alcohol is added until alcohol content reaches 80%-90%, standing is carried out for 12-24 hours, then centrifugation is carried out, absolute ethyl alcohol, acetone and diethyl ether are used for elution in sequence, and crude polysaccharide is obtained; and water is added, the concentration of the crude polysaccharide becomes 3 mg / mL-12 mg / mL, the crude polysaccharide is added into an AB-8 resin column, 8-10 BV of water is used for elution, concentration is carried out until relative density is 15 mg / mL-30 mg / mL, ethyl alcohol is added until alcohol content reaches 80%-90%, standing is carried out for 12-24 hours, then centrifugation is carried out, polysaccharide sediment is obtained, the polysaccharide sediment is washed 2-3 times through absolute ethyl alcohol, acetone and diethyl ether, and the polygala japonica polysaccharide is obtained. The extraction technology is simple and convenient to implement, the extracted polygala japonica polysaccharide can be used for preparing and developing medicine and health care food which can enhance the human body immunologic function, and great economic benefit and social benefit are achieved.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a sunflower seed polysaccharide extracted from sunflower seeds and its application. Background technique [0002] Polysaccharide is a kind of macromolecular substance existing in organisms. In recent years, with the in-depth research on polysaccharides, it has been confirmed that polysaccharides have various biological activities, such as anti-oxidation, anti-tumor, anti-aging, anti-virus, hypoglycemic, hypolipidemic, Improve immunity, etc. And the polysaccharide extracted from the natural product has significant biological activity and is an active ingredient in the natural product. [0003] Guazijin, which first appeared in "Plant Names and Facts", is the whole herb of Polygala japonica Houtt., a plant of Polygala japonica Houtt. Detoxification and pain relief function. For cough with excess phlegm, sore throat; external treatment of bruises, boils, furuncles, and snake bites [1]. M...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A23L1/09
Inventor 杨云张华锋张杰刘炯刘富岗
Owner HENAN UNIV OF CHINESE MEDICINE
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