Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for preparing n-butanol by hydrolyzing lignocellulose and fermenting mixed cellulase crude enzyme liquid

A technology of lignocellulose and mixed cellulose, applied in the field of fermenting n-butanol by hydrolyzing lignocellulose with mixed cellulase crude enzyme liquid, can solve the problem of unbalanced ratio of cellulase system of a single strain, high substrate cost, Cost rise and other issues, to achieve the effect of reducing energy consumption and environmental pollution, high output, and reducing equipment loss

Active Publication Date: 2016-08-24
黑龙江茂全马铃薯生物技术有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention aims to solve the problems of high substrate cost in the existing biological fermentation process of n-butanol production, increased cost caused by the application of commercial enzymes in the lignocellulose hydrolysis process, and reduced enzyme activity caused by the unbalanced ratio of a single strain of cellulosic enzymes.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0022] Embodiment 1: In this embodiment, the method of using mixed cellulase crude enzyme liquid to hydrolyze lignocellulose to ferment and produce n-butanol is realized according to the following steps:

[0023] 1. Alkali pretreatment is adopted to lignocellulose: lignocellulose is mixed with 2% sodium hydroxide solution in a mass ratio of 1:9 to obtain a mixed solution, and the mixed solution is heated at 100° C. After treating for 2 hours, the mixed solution was washed with distilled water until the pH was neutral and dried at 105°C to constant weight, and the obtained residual solid was the pretreated lignocellulose;

[0024] Two, the acquisition of mixed crude enzyme liquid: Trichoderma viride and Aspergillus niger adopt liquid fermentation method respectively under aerobic conditions to obtain Trichoderma viride cellulase crude enzyme liquid and Aspergillus niger cellulase crude enzyme liquid; Then green Trichoderma cellulase crude enzyme liquid and Aspergillus niger cel...

specific Embodiment approach 2

[0033] Specific embodiment two: what this embodiment is different from specific embodiment one is: the crude enzyme liquid of Trichoderma viride cellulase and the crude enzyme liquid of Aspergillus niger cellulase described in step 2 are 3:1 by volume Proportional mix. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0034] Specific embodiment 3: This embodiment differs from specific embodiment 1 or 2 in that the concentration after taking the supernatant in step 3 refers to concentrating to 2 to 4 times the concentration of the supernatant by vacuum distillation. Others are the same as in the first or second embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing n-butanol by hydrolyzing lignocellulose and fermenting mixed cellulase crude enzyme liquid, which relates to a method for preparing n-butanol. The invention aims to solve the problems of high substrate cost in the existing biological fermentation process of n-butanol production, increased cost caused by the application of commercial enzymes in the lignocellulose hydrolysis process, and reduced enzyme activity caused by the unbalanced ratio of a single strain of cellulosic enzymes. . Method: The pretreated lignocellulose was hydrolyzed with a mixed crude enzyme solution derived from Trichoderma viride and Aspergillus niger cellulase, and the obtained sugar solution was added with nitrogen source, inorganic salts and vitamins, then passed through nitrogen to remove oxygen, and inoculated Anaerobic fermentation of Clostridium acetobutylicum seed liquor to produce n-butanol. The invention improves the activity of biological enzymes in the process of enzymatic hydrolysis of cellulose, reduces the cost consumption of substrates and enzymatic hydrolysis in the process of producing n-butanol by biological fermentation, and reduces equipment loss and environmental pollution problems; the mixed crude enzymes in the invention Liquid saccharification and hydrolysis efficiency reaches 75%.

Description

technical field [0001] The invention relates to a method for producing n-butanol by hydrolyzing lignocellulose and fermenting mixed cellulase crude enzyme liquid. Background technique [0002] Currently, the world's energy needs are largely met by fossil fuels. However, the depletion of fossil fuel resources has always been an important factor that plagues the sustainable development of human society. With the development of science and technology, human beings are increasingly dependent on energy for production and living activities. The demand for resources is increasing day by day, therefore, it is imperative to develop a new type of energy. As a new type of energy carrier, n-butanol has good miscibility with gasoline and can be directly applied to existing engines. It has the advantages of high energy density, octane number close to gasoline, and poor hydrophilicity, which is convenient for transportation. , known as a new generation of bioenergy. There are many ways ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/16C12P19/14C12R1/145C12R1/885C12R1/685
Inventor 杨谦王震宇曹广丽宋金柱赵磊冯丽平
Owner 黑龙江茂全马铃薯生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com