Biological fermentation feed and preparation method thereof
A bio-fermented feed and fermentation substrate technology, applied in the field of bio-fermented feed and its preparation, can solve the problems of high cost, high cost of dry substrate, complex production process, etc., and achieve strong pathogenic bacteria, low cost, and operability big effect
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[0034] The present invention also provides the preparation method of biological fermented feed of the present invention, and its steps are as follows:
[0035] a. Isolate and extract Bacillus subtilis, compound yeast, Lactobacillus and Bifidobacterium from healthy pigs, and preserve them;
[0036] b. Mix Bacillus subtilis, compound yeast, lactobacillus and bifidobacteria, and inoculate 3% of the inoculum into a liquid medium composed of 15% molasses and 5% whey powder, and ferment for 2 to 3 days ;
[0037] c. Inoculate the fermented bacterial liquid onto the fermentation substrate, adjust its moisture content, seal and ferment for 5-7 days, and obtain the biologically fermented feed.
[0038] Wherein, the isolation and identification method of Bacillus subtilis, compound yeast, lactobacillus and bifidobacterium can adopt the conventional method in this field, also can adopt following method to carry out:
[0039] Isolation and extraction of Bacillus subtilis: Take 4g of fre...
Embodiment 1
[0046] 1. Preparation of fermentation substrate
[0047] Take 30kg of corn, 10kg of soybean meal, 25kg of washed rice bran, 10kg of wheat bran, 3kg of yeast powder, 0.5kg of sodium chloride, 1kg of calcium hydrogen phosphate, 0.5kg of manganese sulfate, and 0.5kg of magnesium sulfate, and mix well to obtain fermentation substrate A.
[0048] Take 35kg of corn, 15kg of soybean meal, 35kg of washed rice bran, 15kg of wheat bran, 8kg of yeast powder, 2kg of sodium chloride, 5kg of calcium hydrogen phosphate, 3kg of soybean oil, 1.8kg of manganese sulfate, and 1.8kg of magnesium sulfate, and mix well to obtain a fermentation substrate b.
[0049] Take 32kg of corn, 13kg of soybean meal, 30kg of washed rice bran, 12kg of wheat bran, 5kg of yeast powder, 1kg of sodium chloride, 3kg of calcium hydrogen phosphate, 2kg of soybean oil, 1kg of manganese sulfate, and 1kg of magnesium sulfate, and mix well to obtain fermentation substrate C.
[0050] 2. Isolation and identification of str...
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