Method for preparing gold surface boric acid glucose-based sensitive hydrogel and application thereof

A glucose-sensitive, hydrogel technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of poor sensitivity, poor glucose sensitivity, and narrow linear range of glucose response detection, and achieve the effect of good sensitivity

Active Publication Date: 2014-09-03
中科康磁医疗科技(苏州)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above methods usually have the following problems: (1) The pH control of boric acid binding makes it possible for only a small amount of boric acid to react with sugar to form boric acid esters with high stability under the environment of human physiological conditions of pH 7.35 ~ 7.45
(2) Traditional polymer film-forming materials have a narrow linear range for glucose response detection, especially poor sensitivity to high-concentration glucose
(3) Poor sensitivity to glucose at human physiological temperature

Method used

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  • Method for preparing gold surface boric acid glucose-based sensitive hydrogel and application thereof
  • Method for preparing gold surface boric acid glucose-based sensitive hydrogel and application thereof
  • Method for preparing gold surface boric acid glucose-based sensitive hydrogel and application thereof

Examples

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Embodiment 1

[0021] Example 1: (1) Surface pretreatment of gold flakes: put the gold metal flakes in the prepared Piranha reagent (Piranha), ultrasonically clean them for 15 minutes, then ultrasonically clean them with acetone, ethanol, and double-distilled water for 10 minutes, and blow them with N2. dry; wherein, the configuration of Peel's reagent is concentrated H2SO4: H2O2=5: 3 (V: V); (2) amination: dissolve 3-aminopropyltrimethoxysilane in toluene, and the treated gold Place in the above solution under a nitrogen atmosphere, react at 110 °C for 12 h, rinse the gold sheet with absolute ethanol and double distilled water in sequence, and blow dry with N2; the concentration of the silane coupling agent is 3 mmol / L; (3) Polymerization: Add 3-acrylamidophenylboronic acid, ethylene glycol dimethacrylate, diphenyl ketone and acrylamide into dimethyl sulfoxide, mix well, and pipette with a micro syringe 15 uL of the mixed solution was evenly coated on the gold sheet described in step (2), u...

Embodiment 2

[0023]Example 2: (1) Surface pretreatment of gold wafers: place gold-plated silicon wafers in prepared Piranha reagent (Piranha), ultrasonically clean for 30 min, and then ultrasonically clean with acetone, ethanol, and double-distilled water for 10 min, respectively, and argon gas Blow dry; Among them, the configuration of Peel's reagent is concentrated H2SO4: H2O2=7: 3 (V:V); (2) Amination: Dissolve a certain amount of 3-aminopropyl triethoxysilane in ethanol, and The treated gold flakes were placed in the above solution under a nitrogen atmosphere. After reacting at 78 °C for 5 h, the gold flakes were rinsed with anhydrous ethanol and double distilled water in turn, and dried with argon. Among them, the concentration of the silane coupling agent 5 mmol / L; (3) Polymerization: 3-acrylamidophenylboronic acid, N,N-methylenebisacrylamide, 2,4-diethylthioxanthone and acrylamide were added to acetonitrile, mixed After uniformity, use a micro-syringe to pipette 5 uL of the mixed so...

Embodiment 3

[0024] Example 3: (1) Surface pretreatment of gold wafers: place the gold-plated silicon wafers in prepared Piranha reagent (Piranha), ultrasonically clean them for 60 min, then ultrasonically clean them with acetone, ethanol, twice distilled water for 10 min, and helium Blow dry; Among them, the configuration of Peel's reagent is concentrated H2SO4: H2O2=4: 3 (V: V); (2) Amination: Dissolve a certain amount of γ-aminopropylmethyldiethoxysilane in the volume ratio 1:1 ethanol and distilled water, put the treated gold flakes in the above solution, nitrogen atmosphere, react at 50 °C for 12 h, rinse the gold flakes with absolute ethanol and double distilled water successively, and helium Blow dry; where, the concentration of γ-aminopropylmethyldiethoxysilane = 4 mmol / L; (3) Polymerization: 3-acrylamidophenylboronic acid, ethylene glycol bisacryloyl ester, a, a - Dimethoxy-a-phenylacetophenone and acrylamide were added to acetone, and after mixing evenly, pipette 30 uL of the mix...

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Abstract

The invention discloses synthesis of gold surface boric acid glucose-based sensitive hydrogel. The method comprises the following three steps: firstly, carrying out pretreatment on a gold sheet, so that -OH is formed on the surface of the gold sheet, and then successively introducing amino to the surface by using a silane coupling agent; finally adding a 3-acetylaminophenylboronic acid, a cross-linking agent, an initiator and the like; and synthesizing the hydrogel on the surface by ultraviolet light-induced polymerization. The gold surface boric acid glucose-based sensitive hydrogel disclosed by the invention can be applied to recognition, immobilization, sensing, enrichment, separation or detection of glucose, and reversible adsorption and desorption on the glucose can be achieved under physiological pH and temperature conditions of a human body.

Description

technical field [0001] The invention relates to the synthesis of a glucose-sensitive hydrogel based on boric acid on the gold surface, which can realize reversible adsorption and desorption of glucose under the physiological pH and temperature conditions of the human body; it also relates to the hydrogel Application of glue in glucose sensor. Background technique [0002] In the field of biomedicine, sensing systems based on responsive hydrogels are the development trend of precision instruments, especially glucose sensors containing glucose-responsive hydrogels have aroused great interest of researchers. The glucose-sensitive hydrogel is used as a molecular recognition element in a biosensor. When it contacts with a glucose solution, a biological reaction occurs through molecular recognition, and the generated information is converted into an electrical signal by a signal converter. After processing, it can be Realize the analysis and detection of glucose concentration. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/291B01J20/30
Inventor 戴庆
Owner 中科康磁医疗科技(苏州)有限公司
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