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Method for preparing high-yield natamycin by using crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs

A technology of ATCC30772 and natamycin, which is applied in the field of high-yield natamycin by fermenting waste fungus residue of Cryptodinium koiformis ATCC30772, which can solve problems such as undiscovered, reduce pollution, increase production, and reduce production costs

Inactive Publication Date: 2014-09-03
INST OF PLASMA PHYSICS CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the improvement of natamycin fermentation level at home and abroad is mainly through mutagenesis breeding, adjustment of fermentation process, genetic engineering and other means, but there are few reports on increasing the production of natamycin by adding another kind of fermentation waste residue In particular, it is not found that there is an announcement to use Cryptidinium coirii ATCC30772 to ferment waste scum to improve the output of natamycin. This method has both improved the target product yield, reduced production costs, shortened the fermentation cycle, and saved energy. Reuse of waste is beneficial to environmental protection

Method used

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  • Method for preparing high-yield natamycin by using crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs
  • Method for preparing high-yield natamycin by using crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs
  • Method for preparing high-yield natamycin by using crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Preparation of Cryptidium kelpium ATCC30772 fungus residue: activate Cryptidium kelpium ATCC30772 preserved on a slant and put it into a 250ml Erlenmeyer flask containing 50ml of seed medium, culture it at 25℃﹑90r / min for 3 days, then press 10 % of the inoculum was inoculated into a 500ml Erlenmeyer flask containing 100ml of fermentation medium, and cultivated for 9 days at 20°C. The obtained fermentation liquid was centrifuged to obtain bacterial precipitates, washed three times with distilled water, dried in an oven at 80°C, and ground into powder.

[0034] 2) Shake flask seed culture: under aseptic conditions, spread the spores of Streptomyces natalis on the agar slant medium, culture them in the culture room at 28°C, and then configure the cultured Streptomyces natalis experimental strains Sporulation suspension, draw 1ml of this suspension and add it to the triangular flask of seed medium (glucose 10g / L, yeast powder 50g / L, yeast extract 5g / L, pH 7.2), shake the...

Embodiment 2

[0041] 1) The preparation of Cryptidinium koii ATCC30772 fungus residue is the same as in Example 1;

[0042] 2) The shake flask seed cultivation method is the same as in Example 1;

[0043] 3) Shake flask fermentation culture: Inoculate the cultivated seed liquid with 10% inoculum in the Erlenmeyer flask of fermentation medium (glucose 10g / L, soluble dextrin 40g / L, yeast powder 30g / L, pH 7.2) , shake the flask at 220 rpm, 28° C., and cultivate for 120 h. 0.6% sodium propionate was added at 24 hours of fermentation, and 10g / L, 20g / L and 30g / L of Cryptidinium koesili ATCC30772 fermentation waste residue (the rest time is not added). When the fermentation ended, the natamycin output in the fermented liquid reached 4.791g / L, 5.044g / L and 4.841g / L respectively by HPLC method measurement (see figure 2 ).

Embodiment 3

[0045] 1) The preparation of Cryptidinium koii ATCC30772 fungus residue is the same as in Example 1;

[0046] 2) The shake flask seed cultivation method is the same as in Example 1;

[0047] 3) Shake flask fermentation culture: Inoculate the cultivated seed liquid with 10% inoculum in the Erlenmeyer flask of fermentation medium (glucose 10g / L, soluble dextrin 40g / L, yeast powder 30g / L, pH 7.2) , shake the flask at 220 rpm, 28° C., and cultivate for 120 h. 0.6% sodium propionate was added at 24 hours of fermentation, and 10g / L, 20g / L and 30g / L of Cryptidinium koesili ATCC30772 fermentation waste residue (other time is not added). When the fermentation ended, the natamycin output in the fermented liquid reached 4.031g / L, 4.266g / L and 3.987g / L respectively by HPLC method measurement (see image 3 ).

[0048] As can be seen from the above examples, the whole fermentation process is only added once and 10g / L, 20g / L and 30g / L of Cryptidinium koiformis ATCC30772 are added to the ...

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Abstract

The invention discloses a method for preparing high-yield natamycin by using crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs. The method is used for adding the crypthecodinium cohnii ATCC30772 fermented waste mushroom dregs into a fermentation culture medium, thus improving the yield of natamycin. The method disclosed by the invention has the advantages of simple operation, low fermentation cost, energy conservation, environmental protection, relatively significantly-improved yield and the like.

Description

Technical field [0001] The present invention involves Elchalin mold ( Streptomycess natalensis ) The process method of fermentation production, especially the production method that uses hidden arm algae fermentation to produce DHA fermentation waste bacteria to improve the production of nanomycin yield. technical background [0002] Najuromycin is a polymien large -ring endone antibiotics, which is mainly Chainlin mold ( Streptomycess chmanovgensis ), Brown yellow spore chain mold ( Streptomycess gilvosporeus ) And Natal chain mold ( Streptoycess natalensis ) Waiting for fermentation.Its molecule's hydrophobic part, that is, the double -key part of the large ring -to -ring is combined with Van Dehua and the alcohol molecules on the cellular membrane to form antibiotics -alcohol complexes, which destroys the permeability of the cellular membrane, and its pro -pro.The polyol part of the water part of the water forms a water hole on the membrane, which dampen the permeability of th...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/89C12R1/465
CPCY02P20/10
Inventor 卢诗瑶孙立洁袁丽霞肖尚吴金勇陈祥松姚建铭
Owner INST OF PLASMA PHYSICS CHINESE ACAD OF SCI
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