Method for rapid preparation of high density and high purity algae species
A technology of high density and algae species, which is applied in the field of quickly obtaining high-density and high-purity algae species, can solve the problems of low algae yield, waste of energy and nutrition, etc., and achieve rapid proliferation, energy and resource saving, and improvement of photosynthesis efficiency. Effect
Inactive Publication Date: 2014-09-17
李运广
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Problems solved by technology
When cultivating algae according to conventional methods, a single container, a single light intensity, and a single CO are often used. 2 The concentration method not only causes energy and nutrient waste, but also makes the yield of algae low, and the yield of the final harvest is mostly 2 g(dw)L -1 the following
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[0026] Chlorella ( Chlorella vugaris ) were purchased from the Freshwater Algae Collection Center of the Institute of Hydrobiology, Chinese Academy of Sciences.
[0027] The medium used is the preferred medium (KNO 3 0.44gL -1 , NaNO 3 0.60gL -1 , KH 2 PO 4 0.136gL -1 , Na 2 HPO 4 0.142gL -1 , MgSO 4 7H 2 O 0.06-0.3 gL -1 , CaCl 2 0.03gL -1 , NaHCO 3 0.2gL -1 , FeCl 2 7.542mgL -1 , Na 2 EDTA 5mgL -1 ,H 3 BO 3 3.15mgL -1 , MnCl 2 h 2 O 2 mgL -1 ,ZnSO 4 7H 2 O 0.222 mgL -1 , CuSO 4 5H 2 O 0.08 mgL -1 , Na 2 MoO 4 2H 2 O 0.041 mgL -1 , CoCl 2 6H 2 O 0.04 mgL -1 ). Distilled water was used to prepare the medium, and the medium was sterilized at 103.5 kPa, 121°C for 20 minutes, and cooled for later use.
[0028] Pick healthy algal colonies from the plate, inoculate them into a 50ml Erlenmeyer flask, and place them at a speed of 100 rpm -1 Preliminary activation was carried out on a shaker at 25°C under light for 24 hours. The initial...
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Abstract
Belonging to the technical field of algae application, the invention discloses a method for rapid preparation of high density and high purity algae species. The invention involves an efficient culture medium and a preparation method thereof. The efficient culture medium is utilized, algae undergoes shake culture in a triangular flask, air introduction culture in a cylindrical glass tube and a flat plate photobioreactor in order. According to the algae density change in the cultivation process, the light intensity (200-10000lux) is gradually increased. At the same time, the introduction amount of CO2 is regulated, and the pH of the culture solution is maintained at 6.5-8.5, so that the demands of algae for illumination and a carbon source at different periods can be met excellently, the harm of high intensity light on algae growth is avoided, and a best growth state is maintained, thereby improving the photosynthetic efficiency. In the cylindrical glass tube and the flat plate bioreactor, daily production of 1.2g and 0.65g of dry weight algae by per liter of the culture medium can be achieved, and per liter of the culture medium can achieve a final dry algae weight of over 12g and 8g respectively. The method provided by the invention can greatly shorten the large-scale culture cycle of algae and reduce the pollution probability of algae in the culture process, thus being an effective scheme for acquiring high purity algae products.
Description
technical field [0001] The invention belongs to the field of algae application technology, in particular to a cultivation method for rapidly obtaining high-density and high-purity algae species. Specifically, by adjusting light intensity and ventilation, and using different culture devices, high-density and high-purity algae species can be obtained in a short period of time, which can be used as inoculum for large-scale farming to produce open bait, functional food, and high-quality algae for fish, shrimp and shellfish. Value-added secondary metabolites and biodiesel, etc. Background technique [0002] Algae are photoautotrophic organisms with a simple structure, which can efficiently utilize light and CO in the presence of water and some inorganic nutrients. 2 Synthetic organic matter. Algae have high economic value, such as Chlorella is a high-quality plant protein source, Haematococcus pluvialis can obtain a large amount of astaxanthin after being induced, Spirulina is ...
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IPC IPC(8): C12N1/12C12R1/89
Inventor 李运广罗玮汪靓伍阳朱艳红
Owner 李运广