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A strain of Trichoderma simulans tz-11 and its use

A technology of pseudo-Trichoderma konshii, TZ-11, applied in the direction of application, bactericide, fungi, etc., can solve the problems of not mentioned and disclosed, and achieve the effect of low production cost, simple fermentation process and stable storage properties

Active Publication Date: 2016-08-24
ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Trichoderma pseudoconii strain that can effectively control anthracnose provided by the present invention is not mentioned and disclosed in the prior art

Method used

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  • A strain of Trichoderma simulans tz-11 and its use
  • A strain of Trichoderma simulans tz-11 and its use
  • A strain of Trichoderma simulans tz-11 and its use

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 This example illustrates the method for obtaining Trichoderma koningiopsis TZ-11 strain.

[0028] The PDA medium formula is: potato (peeled) 200g / L, glucose 20g / L, water 1L, agar 20g / L, pH natural. Peel the potatoes, cut them into small pieces, add about 1L of water to the pot and boil for half an hour, filter with double gauze, take the filtrate, add glucose, and add water to make up 1L.

[0029] Include the following steps:

[0030] 1) Isolation, purification and preservation of strains.

[0031]Samples were collected from Kyoho grapes in the grape growing area of ​​Jurong City, Jiangsu Province. The collected samples were separated on the PDA plate on the ultra-clean workbench, and single colonies of different forms were picked in time according to the characteristics of the fungal colony to the PDA plate. Above, purify twice, record the strain number, transfer the purified strain to a PDA slant for cultivation, and put it in a 4°C refrigerator for later ...

Embodiment 2

[0036] Embodiment 2 prepares wettable powder with the bacterial strain TZ-11 of preventing and treating anthracnose of the present invention

[0037] The preparation method comprises the following steps:

[0038] (A) Obtaining of solid fermentation culture

[0039] (1) Mix wheat bran and sawdust at a ratio of 3:7 (V / V), add 0.1% (W / W) sucrose and ammonium carbonate, and add water to make the water content 60-65%, Adjust the pH to 7.5-8.0, mix again and put 1.5kg in each bag in a thickened polyethylene plastic bag, tie the bag tightly, and sterilize at 121°C for 60 minutes. Lay 2 sheets of sterilized newspapers on the bottom of the solid fermentation tray in the horizontal tank, add 1.5 kg of sterilized solid fermentation medium, and the thickness of the filling is about 5 cm. Put the TZ-11 strain of Trichoderma pseudoconii transferred into the PDA plate in advance, place it in the incubator for 7 days at 28°C, wash the spores with sterile water, count them with a microscope,...

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Abstract

The present invention relates to a strain Trichoderma pseudoconii ( Trichoderma koningiopsis ) TZ-11 strain, application and preparation method thereof. The wettable powder prepared by the strain through solid fermentation can be used to prevent and control the bacteria caused by the small clusters of bacteria ( Glomerella cingulata ) infection caused by anthracnose, such as capsicum, mango, banana and loquat and many other crop anthracnose control, especially by the small clusters of bacteria ( Glomerella cingulata ) infection caused by grape anthracnose and strawberry anthracnose and other diseases. Field test results showed that the fungal agent had good control effects on both grape anthracnose and strawberry anthracnose, and the strain fermentation process was simple, and the production cost was low; the fungal agent had high content of live spores and stable storage properties.

Description

technical field [0001] The invention relates to a microbial strain and its application, more specifically to a Trichoderma koningiopsis (Trichoderma koningiopsis) TZ-11 strain, its application and its preparation method, belonging to the technical field of biological pesticides. Background technique [0002] Anthracnose of grapes, apples, pears and strawberries caused by the phytopathogenic fungus Glomerella spp. is the main disease of protected agricultural crops. Chemical control is the main control measure for anthracnose. Commonly used chemical agents mainly include carbendazim, propiconazole, bitertanol, imazalil, hexaconazole, azoxystrobin, Kresoxim-methyl, pyrimethanil, difenoconazole, enestroburin, prochloraz, pyraclostrobin, etc. Farmers mostly use the above-mentioned high-efficiency and low-toxicity agents in the control of crop anthracnose, but these agents have a single action site (inhibition of mitochondrial respiratory chain electron transfer). It is very e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 陈宏州肖婷杨敬辉庄义庆
Owner ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
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