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Molecularly imprinted microsphere material based on polymer phase inversion self assembly and application thereof

A technology of molecularly imprinted microspheres and polymers, which can be used in the preparation of alkali metal compounds, other chemical processes, and test samples, and can solve problems such as high prices, reduced specific recognition of imprinted polymers, and difficulty in obtaining protein template molecules. , to achieve the effect of simple preparation process, favorable for large-scale preparation and use, and good reproducibility

Inactive Publication Date: 2014-10-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Since the polymerization reaction often involves conditions such as heat, ultraviolet light, and strong redox reactions that easily denature protein molecules, the specific recognition of imprinted polymers is reduced.
In addition, proteins are often used as template molecules in these blots, but the availability and high price of protein template molecules limit the use of western blotting materials (Sellergren, B. Nature Chemistry, 2, 2010, 7)

Method used

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  • Molecularly imprinted microsphere material based on polymer phase inversion self assembly and application thereof
  • Molecularly imprinted microsphere material based on polymer phase inversion self assembly and application thereof
  • Molecularly imprinted microsphere material based on polymer phase inversion self assembly and application thereof

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Embodiment Construction

[0018] (1) Preparation of molecularly imprinted microspheres based on polymer phase inversion self-assembly

[0019] In the process of preparing epitope-imprinted polyethersulfone (PES) microspheres by self-assembly method, 200mg of PES, 30mg of transferrin epitope (MRLAVGALL), and 23mg of magnetic balls with a particle size of 20nm were dispersed in 800mg of DMAc to form a mixture solution. Then, at room temperature, the PES-transferrin epitope-DMAc pre-assembly system was dropped into water drop by drop to self-assemble into balls. Afterwards, the prepared microspheres were put into water to remove the residual DMAc therein. Then, the template molecules were extracted from the microspheres with methanol / acetic acid solution (alcohol volume percentage 90%), and the leaching solution was changed frequently until no trace was detected on a UV-Vis spectrophotometer (Cary60, Agilent). to template molecules to obtain imprinted microspheres (MIP). Non-imprinted microspheres (NIP...

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Abstract

The invention relates to a molecularly imprinted microsphere material for selective recognition of target protein and a preparation process thereof. According to the molecularly imprinted microsphere material, a high molecular material is used as a base material, a peptide fragment on the target protein is taken as a template molecule, a polymer phase inversion self assembly mode is adopted to prepare a microsphere, and the prepared material is used for selective recognition of protein in a complex sample.

Description

technical field [0001] The invention belongs to the polymer material preparation technology and its application in protein recognition, in particular to a molecularly imprinted microsphere material based on polymer phase inversion self-assembly. Background technique [0002] Molecular imprinting technology is a technology for preparing materials with high selective recognition ability for target molecules by imitating the molecular recognition process in the life process and using artificial synthesis methods. Since Klaus Mosbach et al. published a report on the synthesis of theophylline molecularly imprinted polymers using non-covalent bonds in Nature in 1993, molecularly imprinted materials, as a synthetic highly selective material, have rapidly entered the field of vision of many scientists around the world. At present, great progress has been made in the imprinting of small organic molecules and metal ions, and the experimental methods and evaluation standards are gradua...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28G01N1/40
Inventor 张丽华杨开广刘键熙吴琪邓楠赵群周愿梁振张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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