A suturable hybrid superporous hydrogel keratoprosthesis for cornea

A porous hydrogel, hybrid-type technology for prosthetics, medical materials derived from mammals, immobilized on/in organic carriers, isotropic

Inactive Publication Date: 2014-11-12
THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the matrices described in the prior art have been successfully used to produce corneal implant materials with sufficient strength and biocompatibility for use in corneal replacement surgery

Method used

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  • A suturable hybrid superporous hydrogel keratoprosthesis for cornea
  • A suturable hybrid superporous hydrogel keratoprosthesis for cornea
  • A suturable hybrid superporous hydrogel keratoprosthesis for cornea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Materials and methods

[0048] cell culture. Two cell types, stem cells and committed cells, were analyzed. Human mesenchymal stem cells (MSCs) were maintained in Gibco's α-minimum essential medium (with L-glutamine, without Contains ribonucleosides, does not contain deoxyribonucleosides). The HT-1080 human fibrosarcoma cell line was purchased from ATCC (Manassas, VA). Fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic / antimycotic. Change the medium every 2 to 3 days to remove waste and provide fresh nutrients. Cells in 5% CO 2 and was maintained at 37 °C in the presence of 95% air. cells in 3x10 3 cells / cm 2 The density was plated in tissue culture flasks until a 75-80% confluent monolayer formed. Cells were passaged by incubating cells with 0.25 mg / mL trypsin for 5 minutes and replated at the above densities. All cells used in the experiments herein were between p...

Embodiment 2

[0061] Embodiment 2: swelling ratio

[0062] Since collagen begins to gel rapidly after pH neutralization, it must be loaded into SPH immediately to promote even distribution throughout the SPH. Swelling occurs in less than 1 minute due to the interconnected large-sized pores produced by the SPH fabrication method. Soaking the SPH in the collagen solution allows easy and rapid access of the natural material to the pores by capillary action. Thus, where pre-seeding with cells is desired, the cells can be suspended in the collagen solution immediately prior to uptake. Swelling is determined by the extent and size of interconnected pores. SEM analysis of the pore structures in the three SPHs produced using 100, 200, and 300 mg of sodium bicarbonate revealed two types of pores: larger pores that appeared similar in size and shape in each SPH, and those that formed interconnected. Smaller holes for communication. Apparently, increasing the amount of sodium bicarbonate caused an...

Embodiment 3

[0066] Example 3: Adhesion staining

[0067] In pre-seeded scaffolds, collagen was observed to encourage fibroblasts to spread and form stress fibers in 3-D. Scaffolds without collagen accommodate clumps of round cells that cannot attach to the scaffold. PEGDA is inherently resistant to adhesion. Therefore, it was speculated that the lack of ECM cell binding sites in collagen-free scaffolds caused the circular morphology. After 48 hours, the collagen-free scaffolds were completely cell-free. With nothing to attach to, the cells tend to migrate off the scaffold and attach to the underlying tissue culture plate.

[0068] In contrast, collagen-loaded scaffolds showed cells retained within the scaffold and few, if any, cells attached to the underlying plate. Collagen within the pores of the hydrogel greatly enhances cell spreading and retention in 3-D. Microfilament stress fibers were clearly observed, suggesting that cell adhesion is mediated by available integrin binding si...

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Abstract

The present invention features a hybrid superporous hydrogel scaffold for cornea regeneration and a method for producing the same. The hybrid hydrogel is composed of a superporous poly (2-hydroxyethyl methacrylate) (PHEMA) and poly (methyl methacrylate) (PMMA) copolymer mixed with collagen. The hybrid scaffold can be used as a suturable hybrid corneal implant or keratoprosthesis.

Description

[0001] Introduction [0002] This application claims priority to US Patent Application Serial No. 13 / 284,301, filed October 28, 2011, which is hereby incorporated by reference in its entirety. Background technique [0003] The cornea is an avascular and optically transparent tissue that refracts and filters light before it enters the eye. A transparent cornea is essential for clear vision. The cornea may become opaque after injury, degeneration, or infection. The Vision Share Consortium estimates that corneal blindness affects more than 10 million patients worldwide (Carlsson et al. (2003) Curr. Opin. Ophthalmol. 14(4):192-7). The gold standard treatment is surgical corneal replacement using freshly donated human cadaver corneas. Currently, approximately 40,000 corneal transplants are performed annually in the United States (Eye Bank Association of America. Statistical report 2000), with none performed in nonvascularized "low risk" patients. Complications For the first gra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/14A61L27/52A61F2/14
CPCA61L27/48A61L2430/16A61L27/56A61K35/12A61L27/24A61L27/52A61L27/16C12N11/04A61F2/142C08L33/14C08L33/12A61L27/14
Inventor 迈克尔·卓阿枚利亚·扎兰德尔
Owner THE BOARD OF TRUSTEES OF THE UNIV OF ILLINOIS
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