High efficient and fast culture method of anoectochilus roxburghii tissue cultured seedling

Abstract

The invention belongs to the biological technology field, and specifically relates to a high efficient and fast culture method of anoectochilus roxburghii tissue cultured seedling. The method comprises the following steps: adjusting the hormone concentrations in each stage to the optimum concentrations, then culturing anoectochilus roxburghii seeds in a seed culture medium to form protocorm until small buds start to grow from the protocorm; then transferring the anoectochilus roxburghii buds to a subculture medium until the buds grow into seedlings with 2 to 3 true leaves and 1 to 2 young roots; then transferring the seedlings to an one-step culture medium until the seedlings growth into commercial tissue cultured seedlings with 2 to 3 roots and 4 leaves, wherein the seeding height is not lower than 2.5 cm and the seedling diameter is not smaller than 0.2 cm; and finally transplanting the commercial tissue cultured seedlings to matrix on the seedbed in a greenhouse until the seedlings grow into plants suitable for harvesting. The germination time is shortened, the germination rate is high, the growth of seedling is fast, the pollution and variation are reduced, the tissue cultured seedlings are strong, the leaves and the roots are fully developed; and the non-toxic breeding of anoectochilus roxburghii seedling is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for efficiently and rapidly propagating tissue cultured seedlings of A. Background technique [0002] Golden clematis, also known as golden thread orchid and golden silk grass, is a perennial rare Chinese herbal medicine of the Orchidaceae lip plant. It is used in a wide range of folks, and is known as "King of Medicine", "Golden Grass", "God Grass", "Bird Ginseng" and other laudatory titles. According to the determination of relevant departments, it is found that the amino acid composition, ingredients, content and anti-aging active trace element content of Jinsilian are higher than that of domestic and wild American ginseng. It has been used as folk herbal medicine for hundreds of years, and its demand is increasing. [0003] With the increasing scarcity of wild resources, clematis can only rely on artificial cultivation to meet market demand. [0004] Clema...

AI Technical Summary

Problems solved by technology

However, the prior art has the following disadvantages: because the stem segment propagation needs to undergo dedifferentiation and differentiation processes, and this process is the most prone to variation; at the same time, due to the many times of transfer, slow growth, and long seedling time, the rate of variation High, it is difficult to ensure that the seedlings completely retain the good quality of wild clematis

In addition, due to the complex process of the above-mentioned step-by-step cultivation method, not only the seedling time is long, but also it is easy to cause contamination of the tissue culture seedlings, and lead to an increase in the contamination rate of the tissue culture seedlings.