Biocontrol bacterial strain g58 for preventing and controlling plant fungal diseases and preparation method and application thereof
A technology for plant fungal diseases and biocontrol strains, applied in the field of agricultural biology, can solve the problems of soil microbial zone destruction, frequent plant diseases, chemical pesticide residues, etc., achieve strong colonization ability, improve soil microbial environment, and simple preparation process.
Active Publication Date: 2016-06-15
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology
[0005] In order to solve the above problems, such as frequent occurrence of plant diseases, difficulty in prevention and control, high cost, severe chemical pesticide residues, destruction of soil microbial flora, crop safety, etc., the present invention provides stable effect, strong colonization ability, wide antibacterial spectrum, no environment Contaminated biocontrol strain G58 for controlling plant diseases
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Embodiment 1
[0024] 1. Isolation, screening and identification of biocontrol strain G58
[0025] A plant fungal disease biocontrol strain G58 named Bacillus amylolique faciens G58, isolated from paddy field soil near Zengcheng City, Guangdong Province. The specific separation, screening and identification methods are as follows:
[0026] (1) Isolation of biocontrol strain G58
[0027] Weigh 10.0g of uniformly mixed soil samples, add 90ml of sterile water, and shake for 30min at 30°C at 180rpm to make 10 -1 Bacterial suspension, and then diluted into a gradient of 10 -2 ~10 -6 100 μl of appropriate gradient bacterial suspension was drawn and evenly spread on nutrient agar (10 -5 、10 -6 ) plate, each dilution was repeated three times, and cultured in a constant temperature incubator at 30°C for 1-2 days. According to the difference in physiological properties such as bacterial shape, size, color, etc., pick a single colony and spread it on a plate until a single-form strain is ob...
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The invention discloses a biocontrol fungus G58 for preventing and treating fungal diseases of plants and a preparation method and application thereof. The biocontrol bacteria named Bacillus? amyloliquefaciens G58, accession number CGMCC? No. 8977 The preparation method of the biocontrol agent is to inoculate the activated strain G58 in the NA culture medium and cultivate it for 22-26 hours to obtain the seed solution, and inoculate the seed solution in the fermentation medium and cultivate it for 48-72 hours. h to obtain the fermentation broth, which is the biocontrol agent; the biocontrol agent containing the strain G58 has a significant control effect on rice sheath blight, and the control effect can reach 73.6%. The strain G58 has the characteristics of wide antibacterial spectrum, strong colonization ability, stable effect, low preparation cost, simple application method, environment-friendly, non-toxic, etc., and has good application prospects in the control of plant fungal diseases.
Description
technical field [0001] The invention belongs to the technical field of agricultural biology, and in particular relates to a biocontrol bacterial strain G58 for preventing and treating fungal diseases of plants and a preparation method and application thereof. Background technique [0002] According to statistics from the Food and Agriculture Organization of the United Nations (FAO), at least 10% to 15% of crop losses are caused by crop diseases such as food and vegetables every year, and 70% to 80% of plant diseases are caused by fungi. Several or even dozens of fungal diseases can be found on a crop. Many pathogenic fungi form special tissues or spores that can tolerate changes in the environment and experience severe cold and intense heat. Fungi spread in the field mainly through airflow and water flow; in addition, wind, rain, and insects can also spread fungal diseases. Therefore, pathogenic fungi are difficult to control and are easy to spread widely. Due to the char...
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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 顾文杰卢钰升杨少海解开治李文英蒋瑞萍孙丽丽
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI