A kind of piglet feed anti-stress additive and its preparation method and application
An anti-stress additive, piglet feed technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of insignificant processing effect of crude fiber, reduce salivary cortisol content and blood epinephrine content increase, improve The effect of piglet immunity and yield improvement
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Embodiment 1
[0031] Example 1 The research on the hydrolysis of stalks into glucose
[0032] This example provides a method for hydrolyzing straw into glucose. After the straw is crushed, water is added according to the ratio of material to liquid at 1:0.5 for steam explosion treatment. The steam explosion pretreatment pressure is 1.5-2.0 MPa, and the retention time 120s; after blasting, use pH5.0 buffer to adjust the ratio of material to liquid to 1:5-1:10, add pectinase, hemicellulase and cellulase according to the weight of dry matter, and enzymolyze at 50°C for 24 Hours, after deactivating the enzyme, use a plate and frame filter press or centrifuge to obtain the supernatant; the steam explosion pretreatment pressure is 1.5-2.0MPa, and cannot exceed 2.0MPa, otherwise more furfural by-products will be produced. The reason for steam explosion is to Straw is pretreated. After blasting, the pH can be adjusted to about 5.0-6.0 with a small amount of phosphate buffer.
[0033] The samples ...
Embodiment 2
[0034]Example 2 Effect of reducing sugar concentration of straw hydrolyzate on the growth of fermentation strains
[0035] The enzymatic solution was diluted to obtain sugar solutions with different reducing sugar contents. When the blank was cultured with Lactobacillus plantarum or yeast alone, the OD value was measured with a spectrophotometer. The results are shown in Table 1. It can be seen from Table 1 that when the reducing sugar content of the enzymatic hydrolyzate is higher than 20%, the growth of Lactobacillus plantarum will be significantly affected. The possible reason is that the by-products produced during straw hydrolysis inhibit the growth of Lactobacillus plantarum. After enzymatic hydrolysis, the reducing sugar concentration is maintained below 20% by means of dilution.
[0036] The influence of table 1 reducing sugar concentration on the growth of fermented bacteria
[0037]
Embodiment 3
[0038] Embodiment 3 Lactobacillus plantarum and saccharomyces co-culture research
[0039] Lactobacillus plantarum was purchased from the American Standard Species Bank ATCC number Lactobacillus plantarum ATCC14917. The strain was frozen in 15% glycerol (minus 70-80 degrees Celsius). After two generations of activation in MRS liquid medium, inoculate into the detoxified sugar solution after enzymatic hydrolysis.
[0040] Preparation of Saccharomyces cerevisiae seed medium: transfer Saccharomyces cerevisiae strains to Saccharomyces cerevisiae seed medium for activation, and culture at 30°C for 24 hours; pick activated Saccharomyces cerevisiae strains to inoculate 5mL of Saccharomyces cerevisiae liquid medium, and culture at 30°C for 24 hours , followed by 10% inoculum size expansion. The composition of the seed medium: yeast extract 5g, peptone 10g, sodium chloride 5g, agar 15g, water 1000mL, pH value 7 to prepare Saccharomyces cerevisiae seed medium; Saccharomyces cerevisiae ...
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