Method for preparing conjugated linoleic acid by using rhus chinensis oil

A technology of conjugated linoleic acid and salted wood, applied in the fields of medicine, food and feed, can solve the problems of difficult industrial application, single product, etc. Effect

Inactive Publication Date: 2014-12-03
陈存武
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isomerization of linoleic acid has two methods: chemical isomerization and biological isomerization. Bioisomerization produces conjugated linoleic acid, including enzymatic method and microbial fermentation method. The product is single, high in purity, and the method is relatively advanced. It is still difficult to achieve industrial application; the chemical isomerization method is still widely used in production, in which base-catalyzed isomerization is the main method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The specific operation steps of preparing conjugated linoleic acid by using the oil of sarcocarpus are as follows:

[0032] (1) Preparation of mixed fatty acids containing linoleic acid from the oil of Salmonella

[0033] After 10g sodium hydroxide is dissolved in 300ml ethanol to form sodium hydroxide-ethanol solution, add 100g basil oil, the mass ratio of its basil oil, sodium hydroxide, ethanol is 1: 0.1: 2.4. Under the protection of nitrogen, stir and heat to 70°C, reflux for 3 hours, wait for the saponification of the oil to complete, cool to room temperature, add 15ml of distilled water with 15% of the quality of the oil of Salmonella, dissolve the saponification product, and then add petroleum ether of 20% of the quality of the oil of Salmonella 30ml, extract and remove unsaponified oil to obtain mixed fatty acid salts. Add 2.5ml of hydrochloric acid of 2.5% of the quality of basilisk wood oil to the mixed fatty acid salt, after the mixed fatty acid salt is hydr...

Embodiment 2

[0039] The specific operation steps of preparing conjugated linoleic acid by using the oil of sarcocarpus are as follows:

[0040] (1) Preparation of mixed fatty acids from saltwood oil

[0041] After 15g sodium hydroxide is dissolved in 400ml ethanol to form sodium hydroxide-ethanol solution, add 100g syringa oil, wherein the mass ratio of syringa oil, sodium hydroxide, ethanol is 1: 0.15: 3.2, under nitrogen protection, Stir and heat to 90°C, reflux for 2 hours, and cool after oil saponification. The process of preparing mixed fatty acids from saponification products and solvent recovery are the same as step (1) in Example 1. It has been tested that the hydrolysis rate of Saltwood oil reaches 94.5%;

[0042] (2) Separation and concentration of linoleic acid in mixed fatty acids

[0043] Add 60g of urea to 150ml of ethanol, stir and heat to 50°C and reflux. After the urea dissolves, add 15g of the mixed fatty acid prepared in step (1). The mass ratio of the mixed fatty aci...

Embodiment 3

[0047] The specific operation steps of preparing conjugated linoleic acid by using the oil of sarcocarpus are as follows:

[0048] (1) Preparation of mixed fatty acids from saltwood oil

[0049] After dissolving 12g of sodium hydroxide in 350ml of ethanol to form a sodium hydroxide-ethanol solution, add 100g of kelpwood oil, the mass ratio of kelpwood oil, sodium hydroxide, and ethanol is 1: 0.12: 2.8, under nitrogen protection, stir Heated to 90°C, refluxed for 2.5 hours, and cooled after the oil was completely saponified. The process of preparing mixed fatty acids from saponification products and solvent recovery are the same as step (1) in Example 1. It has been tested that the hydrolysis rate of Saltwood oil reaches 95.6%;

[0050] (2) Separation and concentration of linoleic acid in mixed fatty acids

[0051] Add 70g of urea to 200ml of ethanol, stir and heat to 55°C and reflux. After the urea dissolves, add 16g of the mixed fatty acid prepared in step (1). The mass ra...

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PUM

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Abstract

The invention relates to a method for preparing conjugated linoleic acid by using rhus chinensis oil. The method specifically comprises the following three links: saponification and acidolysis of rhus chinensis oil to prepare mixed fatty acid, urea adduction to separate, concentrate and mix linoleic acid in the fatty acid, isomerization of linoleic acid base to prepare conjugated linoleic acid and the like. The method provided by the invention has the advantages that (1) the method provides new raw materials for preparing conjugated linoleic acid; (2) the method is simple, convenient and economic in preparation process, low in requirement on equipment and strong in operability; (3) a solvent used is non-toxic and part of the solvent can be recycled without generating the environmental pollution; (4) the saponification rate of the rhus chinensis oil reaches over 94%, the purity of linoleic acid subjected to urea adduction reaches 93% and the conversation rate of the conjugated linoleic acid reaches over 90%.

Description

technical field [0001] The invention belongs to the technical fields of medicine, food and feed, and in particular relates to a method for producing conjugated linoleic acid. Background technique [0002] Saltwood ( Rhus chinensis Mill) for Anacardiaceae ( Anacardiaceae ) Salmonella ( Rhus ) small trees, which are distributed in other areas of my country except Xinjiang and Inner Mongolia, and their roots, stems and leaves can be used as medicine. The galls formed by the parasitism of Galla aphids on the leaves of Saltina are the traditional Chinese medicine Galla, so it is also called Galla tree, which has been widely cultivated in Hunan, Guangxi, Guizhou and other places for the production of Galla to extract tannic acid, gallic acid, tannin, etc. Acid and other industrial raw materials. Our research shows that the fruit of Salmonella contains more than 20% oil, and the linoleic acid composition in the oil reaches more than 57%, which has high development value. In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/353C07C57/12
CPCC07C51/353C07C51/02C07C51/412C07C51/48C07C51/50C07C57/12
Inventor 陈存武姚厚军韩邦兴戴军陈乃富张权陈悦
Owner 陈存武
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