Lactobacillus salivarius and pharmaceutical application thereof

A technology of Lactobacillus salivarius, which is applied in the field of microbial biology, can solve the problems of effect influence, whether it can adapt to the environment of human stomach without verification, etc.

Inactive Publication Date: 2014-12-10
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these lactobacilli were not isolated from human stomach, and it was not verified whether they could adapt to the environment of human stomach, which made the development of probiotic lactobacillus exert anti-Helicobacter pylori infection, fixation and inflammation effects in human stomach affected

Method used

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  • Lactobacillus salivarius and pharmaceutical application thereof
  • Lactobacillus salivarius and pharmaceutical application thereof
  • Lactobacillus salivarius and pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] MRS liquid medium (recipe: weigh 55.0g MRS dry powder into 1L water), TTC (2,3,5-triphenyltetrazolium chloride), Skirrow blood plate (recipe: 1L water containing 15.0g peptone, tryptone 2.5g peptone, 5.0g yeast extract, 5.0g NaCl, 20.0g agar powder; add 10mL frozen calf serum, 10% glucose 10mL, antibiotic suspension 1mL (10μg / mL Vancomycin 2500 IU polymyxin B, 5 μg / mL trimethoprim), GES-1 cell culture medium (F12 medium containing 10% fetal bovine serum (Shanghai Xinyu Biotechnology Co., Ltd.)), sterile Oxford cup, bacterial genome extraction kit, MPO Elisa detection kit. Example 1 Isolation and functional identification of Lactobacillus salivarius CGMCC No.8379

[0040] 1. Isolation of 6 Strains of Lactobacillus from Human Stomach

[0041] Methods and steps :

[0042] Gastric mucosal tissue specimens were collected from 21 cases of painless gastroscopy in the Southwest Hospital Affiliated to Third Military Medical University (the specimens were obtained after permi...

Embodiment 2

[0084] Example 2 Verification of antibacterial function of Lactobacillus salivarius CGMCC No.8379 in model organism Kunming mice

[0085] 1. Quantitative detection of Lactobacillus salivarius CGMCC No.8379 against H.pylori in sterile Kunming mice

[0086] Methods and steps :

[0087]32 sterile Kunming mice were divided into 4 groups, 8 in each group, H group (only H. pylori M13 was administered intragastrically), L group (only L. .pylori M13 followed by oral administration of Lactobacillus salivarius CGMCC No.8379), LH group (injection of Lactobacillus salivarius CGMCC No.8379 first and then intragastric administration of H.pylori M13). Take 0.5mL1×10 9 Cfu / ml bacterial solution was administered to sterile Kunming mice twice a day for 2 days, and food and water were resumed 2 hours after the last oral administration. In the double gnotobiotic model, 4 weeks after gavage of the first bacterium, the second bacterium was administered orally, and the Kunming mice were killed ...

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PUM

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Abstract

The invention discloses lactobacillus salivarius which is separated from human gastric mucosa and can be used for preventing and treating stomach diseases caused by helicobacter pylori. The preservation number of lactobacillus salivarius is CGMCC NO.8379. The invention further provides a product for preventing and / or treating stomach diseases caused by helicobacter pylori. The product comprises active components comprising the lactobacillus salivarius. In addition, the invention further provides a product for inhibiting helicobacter pylori. The product comprises active component comprising the lactobacillus salivarius. The application shows that the selected lactobacillus salivarius can not only be well applicable to the human stomach environment and has very high functions of preventing helicobacter pylori growth, adhesion and planting, but also can remarkably reduce inflammation caused by helicobacter pylori in stomach tissue of mice.

Description

technical field [0001] The invention belongs to the technical field of microbial biology, and particularly relates to a Lactobacillus salivarius isolated from human gastric mucosa and used for preventing and treating gastric diseases caused by Helicobacter pylori. Background technique [0002] Helicobacter pylori (H. pylori) is a Gram-negative, unipolar, multi-flagellated, blunt-ended, helically curved microaerophilic bacterium. Studies have shown that it is the main pathogenic factor of chronic gastritis, duodenitis, and peptic ulcer, as well as the main inducing factor of gastric cancer. The World Health Organization has listed it as a class I carcinogen. The infection rate of H. pylori is as high as 50% in the world and as high as 90% in developing countries. Therefore, the prevention and treatment of H. pylori infection has important clinical significance for the protection of human health. [0003] The treatment strategy for H. pylori-related diseases is to eliminate ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P1/04C12R1/225
Inventor 郭刚黄微微杜方川刘开云蒲瑞雪宋阳邹全明
Owner ARMY MEDICAL UNIV
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