Pertussis diagnostic kit and preparation method thereof

Abstract

The invention discloses a pertussis diagnostic kit and a preparation method thereof. The kit comprises a detection probe of a nanogold marker mice anti-human IgA antibody, pertussis bacillus capsular antigen and a dot immunogold concentrated filtration device, wherein the centralized filtration device consists of a plastic capsule, a nitrocellulose membrane added with the detection probe of the nanogold marker mice anti-human IgA antibody and a water absorption layer; water absorption pads are arranged at a groove of the capsule; the nitrocellulose membrane is arranged between the water absorption pads and a cover of the capsule; and the water absorption layer is formed by three to five water absorption pads via stacking. According to the kit disclosed by the invention, the immunogold dots generated by the antigen-antibody reaction are concentrated and amplified again through the concentrated filtration device on the basis of a nanogold amplification technology, so that the detection sensitivity is improved, the limit of detection is enlarged and effective detection can be carried out on the incubation period, the convulsion period and the recovery period.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a pertussis diagnostic kit and a preparation method. Background technique [0002] Pertussis is an acute respiratory infectious disease caused by Bacillus pertussis. The clinical manifestation is paroxysmal spasmodic cough, accompanied by a special inspiratory roar at the end of the cough. The course of the disease is long, up to several weeks or even 3 months, so it is called pertussis. Pertussis cough has been found to cause subconjunctival hemorrhage, rib fractures, urinary retention, hernia, coughing syncope, and vertebral artery dissection. Severe coughing can cause pleural rupture and lead to pneumothorax, especially for children. The harm is very serious. The length of the spasmodic cough is related to the sooner or later treatment, early diagnosis can be treated as early as possible, so the rapid diagnosis of whooping cough is particularly important. [0003] At...

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Problems solved by technology

[0003] At present, there are many methods for medical examination of whooping cough, including white blood cell counting, cell culture, fluorescent antibody staining, serological examination, etc. The cell culture method can only be used as a preliminary diagnosis by isolating colonies and observing the colony shape; fluorescence Antibody staining method is to use nasopharyngeal swab smear, add fluorescent-labeled antiserum, and examine under a fluorescent microscope. 75-80% of early patients are positive. The advantage is that it can be diagnosed quickly, but the disadvantage is poor specificity and false positives. It is only used as an auxiliary culture; serological examination is to do double serum agglutination test and complement binding test. If the antibody titer increases, the diagnosis can be confirmed. Recently, enzyme-linked immunosorbent assay can be used to measure IgM, IgG and IgA antibodies, which is helpful for early diagnosis In addition, the detection methods of B. pertussis or its constituents, such as dot hybridization, PCR, morphology of tissue cell culture, enzyme activity, etc. are still in the stage of laboratory or clinical observation, and have not been widely used.

[0004] The initial diagnosis of B. pertussis is mainly based on isolation and culture. The positive rate of isolation in the incubation period (catarrhal period) can reach 91.5%, but only about 26% in the recovery period. The diagnosis is made by serum slide agglutination or immunoassay with the isolated bacteria and phase I immune serum. Fluorescent staining, so it is mainly limited to phase I detection, and the detection limit is relatively short