Lactic acid bacteria steamed bread processing and room temperature preservation method
A fresh-keeping method and lactic acid bacteria technology, applied in the directions of Lactobacillus, Streptococcus/Lactococcus, food preservation, etc., can solve the problems of unavoidable heat-resistant microbial contamination, decrease in appearance, flavor, taste, and inability to inhibit steamed bread, etc., so as to delay aging. phenomenon, ensuring food safety, reducing the effect of water and nutrient loss
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[0043] B, the preparation of lactic acid bacteria fermented dough
[0044] The activated lactic acid bacteria liquid obtained in step A is centrifuged at a high speed for 5 to 10 min under the condition of a speed of 6000 to 7000 g, and the separated thalline is washed 2 to 4 times with sterile physiological saline to clean the remaining medium components in the bacterial sludge. , obtain the lactic acid bacteria slurry, then dilute it with sterile distilled water, and then obtain the number of colonies to be 2~8×10 10 cfu / ml of active lactic acid bacteria suspension.
[0045] Weigh 1 to 3 parts by weight of the active lactic acid bacteria suspension, add 100 parts by weight of water and 100 parts by weight of flour, mix evenly, and place it in a constant temperature incubator for 22 to 24 hours at a temperature of 36 to 38 ° C to obtain mature Sourdough batter (or "lactobacillus sourdough", "lactic acid fermented dough" or "sourdough").
[0046] The sterile physiological sa...
Embodiment 1
[0078] A. Activation of lactic acid bacteria
[0079] Take 0.5 g of commercial lactic acid bacteria powder, inoculate it into 50 ml of MRS broth medium, and then activate and cultivate for 36 hours at a temperature of 36 to 38 ° C, with an oscillation speed of 10 rpm, to the late stage of the logarithmic growth phase, to obtain activated lactic acid bacteria. liquid;
[0080] B, the preparation of lactic acid bacteria fermented sourdough
[0081] The activated bacteria solution obtained in step A was centrifuged at a high speed for 10 min under the condition of 6500g, and the separated bacteria were washed twice with sterile saline to obtain lactic acid bacteria mud, which was then diluted with sterile distilled water to obtain 6 × 10 10 cfu / ml active bacterial suspension.
[0082] Weigh 2 parts by weight of the active lactic acid bacteria suspension, add 100 parts by weight of water and 100 parts by weight of flour, mix well, and place it in a constant temperature incubator...
Embodiment 2
[0092] A. Activation of lactic acid bacteria
[0093] Same as Example 1;
[0094] B, the preparation of lactic acid bacteria fermented sourdough
[0095] Same as Example 1;
[0096] C, utilize lactic acid bacteria fermented dough to prepare lactic acid bacteria steamed bread
[0097] Add 75 parts by weight of flour, 25 parts by weight of water, 0.5 part by weight of dry yeast, and 50 parts by weight of mature lactic acid bacteria acid fermented sourdough into the mixer, and stir at a speed of 80 to 140 rpm for 3 to 4 minutes, and then at a speed of 180 to 180 Stir at 240 rpm for 1 min, and prepare a mixed dough containing lactic acid bacteria-yeast. Place the prepared lactic acid bacteria-yeast mixed dough in a proofing box with a temperature of 30°C ± 2°C and a relative humidity of 55% ± 5% for 1 hour, and then add 10 parts by weight of flour , to form a uniform dough. Divide the dough into 40g / piece dough blanks, round and shape them, and then place them in a proofing b...
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