Preparation method of monodisperse fluorescent microspheres

A fluorescent microsphere, monodisperse technology, applied in chemical instruments and methods, luminescent materials, etc., can solve the problems of large amount of fluorescent substances added, complicated preparation process, etc., and achieve simple and easy operation, strong chemical stability, and high fluorescence intensity high effect

Active Publication Date: 2014-12-17
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The present invention aims to solve the technical problems of complex preparation process and large amount of fluorescent substances in the existing method for preparing fluorescent microspheres, thereby providing a method for preparing monodisperse fluorescent microspheres

Method used

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  • Preparation method of monodisperse fluorescent microspheres
  • Preparation method of monodisperse fluorescent microspheres
  • Preparation method of monodisperse fluorescent microspheres

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specific Embodiment approach 1

[0024] Specific embodiment one: the preparation method of a kind of monodisperse fluorescent microsphere of this embodiment is carried out according to the following steps:

[0025] 1. Preparation of polystyrene microspheres: Ultrasonicate the glass reactor with acetone, methanol and pure water for 15-20 minutes, then dry it, then add 100-200 mL of ultra-pure water, stir mechanically at 300-400 rpm, and maintain a nitrogen atmosphere, 20 Add 3~10mL of styrene monomer after ~30min, add 8~10mL of 1% potassium persulfate after stirring for 15~20min, then set the speed at 400~500rpm, condense and reflux at a temperature of 60~80°C for 12 ~24 hours to obtain polystyrene microspheres;

[0026] 2. Preparation of fluorescent indicator solution: the concentration of 6-8mg was 1×10 -3 ~1×10 -6 The mol / L fluorescent indicator is evenly dispersed in 10-20mL of organic solvent to prepare a fluorescent indicator solution;

[0027] 3. Preparation of fluorescent microspheres by swelling me...

specific Embodiment approach 2

[0034] Embodiment 2: This embodiment is different from Embodiment 1 in that: in step 1, mechanical stirring is performed at 400 rpm. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, the temperature is 70° C. and the reaction is refluxed for 24 hours. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method of monodisperse fluorescent microspheres and relates to the field of high polymer material synthesis. The preparation method is used for solving the technical problems of the existing fluorescent microsphere preparation method that the preparation process is complicated and the addition amount of fluorescent substances is high. The method comprises the following steps: I, adding a styrene monomer into ultrapure water, stirring, adding 1% potassium persulfate, and carrying out condensation reflux to obtain polystyrene microspheres; II, uniformly dispersing a fluorescent indicator in an organic solvent so as to prepare a fluorescent indicator solution; and III, putting the polystyrene microspheres in a brown test sample bottle and adding the fluorescent indicator solution, carrying out ultrasonic dispersion and centrifugalization, and washing with ultrapure water till no fluorescent indicator is left on the surfaces of the microspheres. The method disclosed by the invention is simple and easy to operate and small in use level of the indicator, and the obtained fluorescent microspheres are high in fluorescence intensity, good in light resistance, strong in chemical stability and at a monodisperse state. The particle size of the fluorescent microspheres prepared by the preparation method is 100-400nm. The preparation method is applied to the field of preparation of fluorescent microspheres.

Description

technical field [0001] The invention relates to the field of polymer material synthesis. Background technique [0002] Fluorescence refers to the visible light of different colors and intensities emitted by these substances when ultraviolet rays irradiate certain substances; when the ultraviolet rays stop irradiating, the light emitted by the substances also disappears quickly, which is called photofluorescence. [0003] Most fluorescent indicators belong to organic dyes, polycyclic aromatic hydrocarbons, and metal-organic complexes. Metal-organic complexes have high fluorescence emission intensity and good optical stability, and are excellent fluorescent indicators. Porphyrins and metalloporphyrins are a class of macrocyclic compounds that widely exist in nature. They are dark solids with high melting points, poor water solubility, and good chemical and thermal stability. Because porphyrin is a conjugated system structure with a chromophore, it is an ideal compound as a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L25/06C08K5/00C08J7/00C09K11/06C08F112/08
Inventor 张科赵九蓬李文杰李垚
Owner HARBIN INST OF TECH
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