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A method of improving the yield of Ganoderma lucidum laccase by using waste paper shreds

A technology for pulverized material and waste paper, which is applied in the field of using the pulverized material of waste paper to increase the yield of Ganoderma lucidum laccase, can solve the problems of increasing the difficulty of post-processing of fermentation liquid, failing to exert the value of waste paper resources, and low level of recycling and utilization of waste paper, etc. The effect of short cycle, simple preparation method and low cost

Inactive Publication Date: 2017-12-29
HENAN NORMAL UNIV
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Problems solved by technology

These inducers are generally toxic, and the addition increases the difficulty of post-processing of the fermentation broth, and is likely to cause new secondary pollution
[0004] Waste paper refers to all kinds of paper products that are discarded after being used in production and life. The recycling level of waste paper in my country is low, and a large amount of recycled waste paper is also processed into low-grade products such as cardboard and toilet paper by small enterprises with backward technology. Giving full play to the resource value of waste paper will also bring serious secondary pollution

Method used

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  • A method of improving the yield of Ganoderma lucidum laccase by using waste paper shreds

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Embodiment

[0019] 1. Preparation of waste paper shreds

[0020] Soak the shredded waste paper (waste newspaper, book paper and kraft paper) in tap water for 5-8 hours, the moisture content of the waste paper is 50%-80%, dry at 100°C to constant weight, and crush it with a shredder As a powder, waste paper shreds were obtained.

[0021] 2. PDA plate culture medium

[0022] Potatoes 300g, glucose 20g, agar 18g, distilled water to make up to a total volume of 1L, sterilized for later use.

[0023] 3. Seed medium

[0024] 100g cornmeal, 100g bran, 25g peanut shell powder, KH 2 PO 4 10g, MgSO 4 5g, make up to a total volume of 1L with distilled water, and sterilize for later use.

[0025] 4. Fermentation medium

[0026] Control medium 1: cornmeal 100g, bran 10g, peanut shell powder 25g, KH 2 PO 4 10g, MgSO 4 5g, make up to 1L with distilled water, and sterilize for later use.

[0027] Control medium 2: cornmeal 100g, bran 30g, peanut shell powder 25g, KH 2 PO 4 10g, MgSO 4 ...

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Abstract

The invention discloses a method for increasing the yield of ganoderma lucidum laccase by using waste paper crushing materials and belongs to the technical field of enzyme preparations for industrial purposes. According to the technical scheme, the method comprises the following four steps: preparation of the waste paper crushing materials, preparation of a ganoderma lucidum seed solution, liquid fermentation of ganoderma lucidum and preparation of a crude enzyme solution. The waste paper crushing materials are wide in source and low in cost, and the preparation method is simple. No toxic material induction exists in the enzymatic synthesis process, post processing is simple, and environment is not polluted. Enzyme is high in yield and short in cycle, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for preparing an industrial enzyme preparation, in particular to a method for increasing the yield of ganoderma lucidum laccase by using crushed waste paper. Background technique [0002] Laccase (1accase) is a class of copper-containing polyphenol oxidase, which has broad substrate specificity and can oxidize a variety of phenols and aromatic amines. It is widely used in paper industry, food processing, dye decolorization and degradation, Sewage treatment, biosensors and immunoassays have broad application prospects [Susana Rodríguez Couto, José Luis Toca Herrera. Industrial and biotechnological applications of laccases: A review. Biotechnology Advances. 2006; 24(5): 500-513]. In recent years, it has become a research hotspot in the interdisciplinary field of bioengineering and environmental science. [0003] Laccase widely exists in plants, higher fungi, insects and bacteria, among which white-rot fungi in basidiomy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12R1/645
CPCC12N9/0061C12Y110/03002
Inventor 王海磊刘宇峰杨玉华李平
Owner HENAN NORMAL UNIV
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